Kinetics of β-lactam antibiotics synthesis by penicillin G acylase (PGA) from the viewpoint of the industrial enzymatic reactor optimization

Giordano, Roberto de Campos; Ribeiro, M. P. A.; Giordano, R. L. C.

doi:10.1016/j.biotechadv.2005.05.003

Cited by 76 publications

(48 citation statements)

References 83 publications

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“…Although PA has intensively been studied due to this industrial importance (e.g. Alkema et al, 2000, Arroyo et al, 2003, Giordano et al, 2006, Kheirolomoom et al, 2001, published parameter estimates vary drastically (Alkema et al, 1999). To investigate if such deviations may be caused by using different analysis approaches, PA was chosen for the comparison of the computer programs.…”

Section: Resultsmentioning

confidence: 99%

Model-based experimental analysis of enzyme kinetics in aqueous–organic biphasic systems

Zavrel

Schmidt

Michalik

et al. 2007

Journal of Biotechnology

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Section: Resultsmentioning

confidence: 99%

Model-based experimental analysis of enzyme kinetics in aqueous–organic biphasic systems

Zavrel

Schmidt

Michalik

et al. 2007

Journal of Biotechnology

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“…The enzyme has very broad substrate specificity with utility to the production of semi-synthetic penicillins and β-lactam antibiotics. [98,99] The enzyme can resolve racemic mixtures of chiral compounds with excellent stereochemistry for a range of substrates. The catalytic versatility of the enzyme has been extended by immobilizing PGA onto chromatography supports and using the enantiomeric selectivity of the enzyme to resolve racemic mixtures.…”

Section: Preparation Of Penicillin Analoguesmentioning

confidence: 99%

Silica-Immobilized Enzyme Reactors

Luckarift¹

2008

Journal of Liquid Chromatography & Related Technologies

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Standard Form 298 (Rev. 8/98) REPORT DOCUMENTATION PAGEPrescribed by ANSI Std. Z39.18 Form Approved OMB No. 0704-0188The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, The effect of various silica matrices and immobilization techniques upon the enzymatic properties and stability of the biocatalysts is considered. In addition, reports in which the carrier matrix is biologically-derived silica are highlighted as an alternative and versatile technique that provides advantageous recovery, reuse and reproducibility. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) SPONSOR/MONITOR'S REPORT NUMBER(S)

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“…However, it is still not economically competitive with the fine-tuned conventional chemical process (Giordano et al, 2006). In order to reduce costs of the enzymatic route is essential increasing the yield and selectivity (synthesis/hydrolysis ratio).…”

Section: Introductionmentioning

confidence: 99%

Feasibility of Using Different Ionic Liquids for the Enzymatic Synthesis of Amoxicillin

Pereira¹,

Kopp²,

Duro³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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-Industrially, amoxicillin has been produced by chemical processes. But, the enzymatic synthesis catalyzed by penicillin G acylase (PGA, E.C.3.5.1.11) is more attractive from the environmental point of view. However, an obstacle for the industrial application of the enzymatic route is the limited product yield due to undesirable hydrolytic reactions. This drawback can be minimized by reducing water activity in the reaction medium. Ionic liquids (ILs) have emerged as an alternative to conventional organic media. This study assessed the feasibility of use of different ILs, protic (PILs) and aprotic (AILs), in the enzymatic synthesis of amoxicillin, by testing the stability of reaction substrates (6-aminopenicillanic acid and phydroxyphenylglycine methyl ester) in these solvents. These compounds were completely stable in AILs. Besides, PGA was active in AILs. In contrast, none of the evaluated PILs was inert for these substrates making unfeasible the use for this enzymatic process.

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Kinetics of β-lactam antibiotics synthesis by penicillin G acylase (PGA) from the viewpoint of the industrial enzymatic reactor optimization

Cited by 76 publications

References 83 publications

Model-based experimental analysis of enzyme kinetics in aqueous–organic biphasic systems

Model-based experimental analysis of enzyme kinetics in aqueous–organic biphasic systems

Silica-Immobilized Enzyme Reactors

Feasibility of Using Different Ionic Liquids for the Enzymatic Synthesis of Amoxicillin

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