Silica-Immobilized Enzyme Reactors

Abstract

Standard Form 298 (Rev. 8/98) REPORT DOCUMENTATION PAGEPrescribed by ANSI Std. Z39.18 Form Approved OMB No. 0704-0188The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, The effect of various silica matrices and immobilization techniques upon the enzymatic properties and stability of the biocatalysts is considered. In addition, reports in which the carrier matrix is biologically-derived silica are highlighted as an alternative and versatile technique that provides advantageous recovery, reuse and reproducibility. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) SPONSOR/MONITOR'S REPORT NUMBER(S)

Chitosan/siloxane hybrid polymer: synthesis, characterization and performance as a support for immobilizing enzyme

“…1 Moreover, immobilization may be used to improve other enzyme features, such as increasing the activity, 2 decreasing inhibitions, 3 modulating selectivity and specificity 4 or improving the enzyme behavior in synthetic processes. 5 Particularly for lipase, 6 the immobilization state is required to attain high product yield so it is not surprise that its immobilization has been the object of intensive investigation.…”

Immobilisation of enzymes on mesoporous silicate materials

“…1) for the silicate, mesoporous cellular foam (MCF), will be used to illustrate the different 30 results that can be obtained. MCF is a mesoporous silicate material with ink bottle pores which contain spherical cells (body of the ink bottle) that are 45 interconnected via narrower channels (neck of the ink bottle). Barret-Joyner-Halenda analysis of the adsorption and desorption isotherms yield average pore diameters of 29 and 22 nm, respectively.…”

“…Zeolites are among the most commercially important porous materials and are in widespread use in a range of industrially 45 important processes 13 . They are of particular interest as shape selective catalysts where the internal spaces of the zeolites are of similar dimensions to the reactant and product molecules, with larger molecules unable to penetrate into the pores where catalysis occurs.…”

“…9, 10 An early example of enzyme immobilisation is that of glucose oxidase on a membrane support which was successfully 45 developed as biosensor for clinical use 11 . A wide range of immobilised enzymes has been used for applications in biocatalysis 2,3,5,6,12 while more recently, the use of immobilised enzymes in biofuel cells has attracted significant interest in the development of implantable self-powered medical devices 9 .…”

“…40 Evidence for the encapsulation of an enzyme in the pores of MPS was obtained by using small-angle neutron scattering to show that cross-linked glucose oxidase and chloroperoxidase were present in the pores of the support. 36 In an elegant experiment, Salis et al have provided direct experimental 45 evidence of the presence of lysozyme within the pores. 37 The method (Fig.…”