Kinetics of the reaction of human renin with natural substrates and tetradecapeptide substrate

Favre, L.; Vallotton, Michel B.

doi:10.1016/0005-2744(73)90430-0

Cited by 22 publications

(9 citation statements)

References 16 publications

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“…Differences in the conditions of incubation or assay methods arc not obviously the cause of this and other factors are possible. The problem is exemplified by the recent report of Favre and Vallotton (1973) that affinity of humau renin for renin substrate varies inversely with reaction velocity. Such a fiuding defies any simple explanation including that offered by the authors of the presence of a competitive inhibitor in the plasma snbstrate preparation.…”

Section: Discussionmentioning

confidence: 99%

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

Skinner

Dunn

Mazzetti

et al. 1975

Aust J Exp Biol Med

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Summary. Sheep plasTiia renin substralf was purified 1,200-fold l>y usinjj nephrfctomised sheep plasma, followed by DEAE-Sephadex chromatojijraphy and gel filtration. Th? purified substrate contained 8 nji angiotensin Il/nig protein and had an estimated molecular vveijjht of 52,000. The kinetic characteristics of the purified substrate were identical both to those of tuipurified nephrectomised sheep plasma and to normal sheep plasma substrates. At pH 7-5, K,,, of the human renin-sheep substrate reaction was 0-29 [iM and for sheep reninsheep substrate. 2-0 fiM. Sheep substrate was .susceptible to peptic diHeslion with generation of pepsitenshi.Hunuin renin substrate was less readily purified. DEAE-Sephadex chromatography of plasma from pregnant women at 36-40 weeks' gestation produced a 70-fold increase in purity (0-9 ng angiotensin Il/mg protein). No further increase was achieved with gel filtration. Human renin substrate behaved as a larger (mol. wt. 82.000) more anionic protein than sheep substrate and was resistant to the proteolytit actions of both pepsin and shi-ep renin. K,,, for the human renin-human substrate reaction was high and conid not be accurately detennined (range 3-8 nM. mean 5-7 ^iM). The presence of human substrate in a human rcnin-sheep substrate system did not alter the measured initial velocity.In both shet'p and man, the nonnal concentration (»f ri^nin substrate is considerably less than K,,, and must therefore be considered a dtterminant of angiotensin production rate j>i vivo.

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Section: Discussionmentioning

confidence: 99%

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

Skinner

Dunn

Mazzetti

et al. 1975

Aust J Exp Biol Med

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“…PRC was cal culated from the PRA and PRS data using the integrated form of the Michaelis-Menten equation as used by H aas and G oldblatt [17] and by Gould and G reen [18] to describe the kinetics of renin. The kinetic parameters obtained in our laboratory were used: K m= 800 pmol/ml and velocity constant (kg) = 2,080 GU/ml [19]. Plasma aldosterone was de termined by a radioimmunoassay [20] using the antiserum No.088 from the N1H.…”

Section: Methodsmentioning

confidence: 99%

The Renin-Angiotensin-Aldosterone System in Mother and Fetus at Term

Godard¹,

Gaillard²,

Vallotton³

1976

Nephron

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Plasma renin activity, renin substrate, angiotensin II, aldosterone and cortisol were measured concurrently and renin concentration calculated in plasma from mothers during labor and delivery, from cord and from newborn infants. The renin-angiotensin-aldosterone system was found strongly stimulated in both mother and fetus. The high values of plasma renin activity in fetus were due exclusively to the high renin concentrations the substrate concentration being normal. In the mother, however, the markedly elevated renin substrate resulted in a doubling of relative values of renin activity compared to renin concentration. Therefore gradients of renin and renin substrate across the placenta are established, but the resulting renin activity is similar on both sides and the levels of generated angiotensin II are also nearly indentical with a good correlation between these last parameters. Aldosterone is as elevated in mother as in fetus whereas cortisol, due to its binding to transcortin, is twice as high in mother as in fetus. No correlation was found between renin activity or concentration of angiotensin II and aldosterone or cortisol indicating that other factors controlling aldosterone are involved.

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“…It is generally agreed that the normal concentration of reniu substrate in man is very close to the nominal values of the Km of renin. Thus, the kinetics of renin in vivo and in vitro is not of zero order, but at best of mixed zero and first orders (Favre and Vallotton, 1973). Nevertheless, from such a normal value any elevation of renin substrate could at the most, everything else being equal, double the plasma renin activity.…”

Section: Discussionmentioning

confidence: 96%

“…Since the true renin concentration by internal calibration could not be measured in the present case for lack of material, it was derived by application of the integrated Michaelis-Menton equation (Gould and Green, 1971 ;Haas and Goldblatt, 1967). For this purpose, the values of K~ and velocity constant found in our laboratory were applied (Favre and Vallotton, 1973). Table 1 shows the plasma renin concentration thus obtained.…”

Section: Discussionmentioning

confidence: 99%

Epithelial liver hamartoma, systemic arterial hypertension and renin hypersecretion

Cox

Paunier

Vallotton

et al. 1975

Virchows Arch. A Path. Anat. and Histol.

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The case of a 14-year old girl presenting with headaches, severe progressive hypertension and high plasma renin levels, in whom a voluminous epithelial liver hamartoma or adenoma was discovered at surgery is documented. The morphological characteristics of the hamartomatous abnormality are described and evidences are put forward which would suggest that the liver lesion might have been the site of the abnormal renin production which was responsible for the systemic arterial hypertension.

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Kinetics of the reaction of human renin with natural substrates and tetradecapeptide substrate

Cited by 22 publications

References 16 publications

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

The Renin-Angiotensin-Aldosterone System in Mother and Fetus at Term

Epithelial liver hamartoma, systemic arterial hypertension and renin hypersecretion

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