Kinetics of the Bioproduction of Succinic Acid by Actinobacillus succinogenes from Oil Palm Lignocellulosic Hydrolysate in a Bioreactor

Luthfi, Abdullah Amru Indera; Jahim, Jamaliah Md; Harun, Shuhaida; Tan, Jian Ping; Manaf, Shareena Fairuz Abdul; Shah, Siti Syazwani Mohd

doi:10.15376/biores.13.4.8279-8294

Cited by 22 publications

(5 citation statements)

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“…Compared to the enzymatic hydrolysis of OPF bagasse by Luthfi et al [28], the acid hydrolysis of OPF bagasse reported by Tan et al [75] generated lower total fermentable sugar, 44.2 g/L. The study utilised the frond section 1.5 to 3 m away from the trunk, which recorded the highest sugar content compared to other portions of the frond.…”

Section: Pretreatment and Hydrolysis Of Fermentable Sugars From Oil P...mentioning

confidence: 90%

“…Conversely, the bacterial fermentation technology can be adopted to generate succinic acid from sustainable feedstock, such as biomass and waste materials from the agricultural sector [27]. The bacterial strains that have been evaluated and commercialised for the purpose include Actinobacillus succinogenes [28], Anaerobiospirillum succiniciproducens [29], Mannheimia succiniciproducens [30], and recombinant Escherichia coli [31], Corynebacterium glutamicum is another genetically-modified bacterial strain that demonstrated enhanced efficiency in succinic acid production [32].…”

Section: Polybutylene Succinate (Pbs)mentioning

confidence: 99%

“…Luthfi et al [28] documented a maximum total fermentable sugar content of 72.7 g/L from OPF bagasse hydrolysate by adopting alkaline pretreatment with 4% (w/v) NaOH at a solid-to-liquid ratio of 1:10 (w/v) at 100 • C for 58 min. Subsequently, enzymatic hydrolysis employing cellulose and hemicellulose at 50 • C for 72 h was performed.…”

Section: Pretreatment and Hydrolysis Of Fermentable Sugars From Oil P...mentioning

confidence: 99%

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Succinic Acid Production from Oil Palm Biomass: A Prospective Plastic Pollution Solution

et al. 2023

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Plastic pollution has placed a significant emphasis on the need for synthesising bioplastics, such as polybutylene succinate (PBS), which is derived from succinic acid. Furthermore, environmental concerns and the depletion of non-renewable fossil fuels have initiated an interest in exploring the biotechnological route of succinic acid production via fermentation. Consequently, oil palm biomass might be a prospective substitute for the costlier pure carbon source, which is more sustainable and cost-effective due to its abundance and high lignocellulosic content. The current review focuses on the potential of oil palm biomass utilisation to synthesise succinic acid and its associated bioplastics. The pretreatment and hydrolysis of various oil palm biomass and studies on bioplastics generation from oil palm biomass are also discussed. This review also identified the challenges of manufacturing succinic acid from oil palm biomass and included several recommendations.

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Section: Pretreatment and Hydrolysis Of Fermentable Sugars From Oil P...mentioning

confidence: 90%

Section: Polybutylene Succinate (Pbs)mentioning

confidence: 99%

Section: Pretreatment and Hydrolysis Of Fermentable Sugars From Oil P...mentioning

confidence: 99%

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Succinic Acid Production from Oil Palm Biomass: A Prospective Plastic Pollution Solution

et al. 2023

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“…Further, the saturation constant (K s =75.17) clearly indicates that substrate uptake is by the passive diffusion mechanism, which is common in yeast cell factory. Luthfi et al [45] succinogenes ZT-130 [45]. Pateraki et al [65] used modified Monod's model to identify both substrate and product inhibition of A. succinogenes and Basfia succiniciproducens in a xylose-rich spent sulphite hydrolysate.…”

Section: Discussionmentioning

confidence: 99%

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Prabhu

Amaro

Lin

et al. 2020

Preprint

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Background Xylose is a most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently metabolize xylose as sole carbon source. Yarrowia lipolytica is a non-conventional yeast to produce industrially important metabolites, and it is able to metabolize a large variety of substrates including both hydrophilic and hydrophobic carbon sources. However, Y. lipolytica lacks effective metabolic pathway for xylose uptake and only scarce information is available on utilization of xylose. For the economically feasible of LCB-based biorefineries, effective utilization of both pentose and hexose sugars is obligatory. Results In the present study, succinic acid (SA) production from xylose by Y. lipolytica was examined. To this end, Y. lipolytica PSA02004 strain was engineered by overexpressing pentose pathway cassette comprising of xylose reductase ( XR ), xylitol dehydrogenase ( XDH ) and xylulose kinase ( XK ) gene. The recombinant strain exhibited a robust growth on xylose as sole carbon source and accumulated SA (3.8 g/L) with a yield of 0.19 g/g in shake flask studies. Substrate inhibition studies revealed a marked negative impact on cell growth and product formation above 60 g/L xylose concentration. The modelling based on inhibition kinetics revealed that Aiba model showed better fit with experimental data, which resulted the correlation coefficient (R 2 ) of 0.82 and inhibition constant (K I ) 88.9 g/L. The batch cultivation of recombinant strain in bioreactor resulted in a maximum biomass concentration of 7.3 g/L and SA titer of 11.2 g/L with the yield of 0.18 g/g. Similar results in term of cell growth and SA production were obtained with xylose-rich hydrolysate derived from sugarcane bagasse. The fed-batch fermentation yielded biomass concentration of 11.8 g/L (OD 600 : 56.1) and SA titer of 22.3 g/L with a gradual decrease in pH below 4.0. Acetic acid was obtained as a main byproduct in all the fermentations. Conclusion The recombinant strain displayed potential bioconversion of xylose to succinic acid. Further this study provided a new insight on conversion of LCB into value-added products. To the best of our knowledge, this is the first study on SA production by Y. lipolytica using xylose as a sole carbon source.

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“…Ginkel and his co-worker has demonstrated that the presence of the appropriate concentration of substrate during hydrogen fermentation could increase the ability of the hydrogen-producing bacteria to produce maximum amount of hydrogen [2]. However, the optimal amount of substrate and micronutrients need to be determined or otherwise the presence of excess amount of it will inhibit the process [3]. The presence of optimal substrate concentration and micronutrients are able to improve the metabolic reaction of microbes which have a relationship with microbial growth.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Study on the Effect of Substrate and Micronutrient Inhibition during Anaerobic Fermentation of Biohydrogen

Engliman¹

2022

ijcsrr

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There are several factors that influence the production of biohydrogen by dark fermentation including inoculum seeds, type and concentration of substrate, pH, temperature, presence of micronutrient and reactor configuration. Previous research has proven that the concentration of substrate and the presence of micronutrient will influence the yield and productivity of biohydrogen production. However, improvement of yield and productivity of the process can only be achieved once the system is under the optimum amount of substrate and micronutrient. Therefore, the best way to determine the effect of substrate concentration and presence of micronutrient is through kinetic study that was done using Monod model along with Andrews model. Besides that, the substrate inhibition effect also will be evaluated to determine the maximum substrate that needs to be supplied for maximum hydrogen production, and thus supplied the information for economic feasibility for fermentation process. In the meantime, the inhibition effect of adding the iron nanoparticles also had been evaluated in order to understand the interaction effect between iron nanoparticles and bacteria in term of catabolism reaction. It was found that increasing the substrate concentration more than 10 g/l will cause the inhibition to the system, in which it will slow down the reaction process and reduced the production of hydrogen. While the presence of iron NPs more than its optimum value (200 mg/l) will inhibit the bacterial growth and hence, affect the hydrogen production. For both cases, when the inhibition occurred at the respective concentration, it was found that the metabolic pathway was shifted to produce more hydrogen-consuming metabolite such as propionate acid, and thus, dropped the hydrogen production.

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Kinetics of the Bioproduction of Succinic Acid by Actinobacillus succinogenes from Oil Palm Lignocellulosic Hydrolysate in a Bioreactor

Cited by 22 publications

References 0 publications

Succinic Acid Production from Oil Palm Biomass: A Prospective Plastic Pollution Solution

Succinic Acid Production from Oil Palm Biomass: A Prospective Plastic Pollution Solution

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Kinetic Study on the Effect of Substrate and Micronutrient Inhibition during Anaerobic Fermentation of Biohydrogen

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