Kinetics of taxol production and nutrient use in suspension cultures of Taxus cuspidata in shake flasks and a Wilson-type bioreactor

Pestchanker, Luis J.; Roberts, Susan C.; Shuler, Michael L.

doi:10.1016/0141-0229(95)00243-x

Cited by 45 publications

(17 citation statements)

References 14 publications

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“…During culturing, the excretion of both taxanes in cells grown in flasks and in the bioreactor exceeded on average 72% of the total compound content which is consistent with results obtained by Navia-Osorio et al (2002) and Pestchanker et al (1996) although Wickremesinhe & Arteca (1994) reported only a 10% release of paclitaxel in suspension culture of Taxus · media. It was earlier demonstrated that at low inoculum sizes of 1.5 and 2.0 g dry wt l )1 the extracellular paclitaxel concentration was relatively higher (Wang et al 1997) which is in accordance with our results.…”

Section: Resultssupporting

confidence: 92%

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Taxane Production in Suspension Culture of Taxus × Media var. Hicksii Carried Out in Flasks and Bioreactor

Sykłowska-Baranek

Furmanowa

2005

Biotechnol Lett

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Paclitaxel and 10-deacetylbaccatin III (10-DAB III) were produced in suspension cultures of Taxus x media var. Hicksii grown in shake-flasks and in a 7-l bioreactor reaching, in the bioreactor, 4.4 mg l(-1) (on day 14) and 37.5 mg l(-1) (on day 11). In shake-flasks the highest total content of paclitaxel and 10-DAB III was 7.3 mg l(-1) (on day 4) and 8.8 mg l(-1) (on day 18). Phenylalanine, at 0.05 mM, increased paclitaxel accumulation in cells cultivated in bioreactor and flasks 30-fold and 9-fold (from 0.02 mg l(-1) to 0.6 mg l(-1) and to 0.2 mg l(-1), respectively). The 10-DAB III content in cells from flasks was increased from 0.4 mg l(-1) to 1.6 mg l(-1).

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Section: Resultssupporting

confidence: 92%

“…Data recorded during cultivating cells in bioreactor might be contributed to higher cell damage by sharing as the medium stirring was set at 400 rpm. The similar growth pattern of suspension culture performed in flasks and bioreactors was also reported by Pestchanker et al (1996).…”

Section: Resultssupporting

confidence: 65%

Taxane Production in Suspension Culture of Taxus × Media var. Hicksii Carried Out in Flasks and Bioreactor

Sykłowska-Baranek

Furmanowa

2005

Biotechnol Lett

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“…This is also applicable to baccatin III using the Wave reactor system, in which the content of 7.78 mg.L À1 was 2.4-fold higher than in the shake-flask culture. by day 20 (Pestchanker et al, 1996), 2.94 mg.L À1 by day 21 (Vanek et al, 1999), 3.0 mg.L À1 by day 27 (Son et al, 2000), 21.04 mg.L À1 by day 24 (Navia-Osorio et al, 2002), and 21.12 mg.L À1 by day 20 . Ketchum et al (1999) cite a paclitaxel content of 117 mg.L À1 with a volumetric productivity of 23.4 mg.L À1 .…”

Section: Discussionmentioning

confidence: 99%

Effects of immobilization by entrapment in alginate and scale‐up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata

Bentebibel

Moyano

Palazón

et al. 2005

Biotech & Bioengineering

101

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Paclitaxel and baccatin III-producing cells of Taxus baccata were immobilized within Ca(2+)-alginate beads. Under established optimum conditions for the biosynthesis of both taxanes, the yields of paclitaxel and baccatin III in shake-flask cultures of free cells increased by factors of up to 3 and 2, respectively, in the corresponding cultures of immobilized cells. Although the scale-up from shake-flask to bioreactor culture usually results in reduced productivities when both free and immobilized cells were grown in the same optimum conditions in three different bioreactor types (Stirred, Airlift, and Wave) running for 24 days in a batch mode and with the system optimized in each case, there was a considerable increase in the yields of paclitaxel and baccatin III. Among the reactors, the Stirred bioreactor was the most efficient in promoting immobilized cell production of paclitaxel, giving a content of 43.43 mg.L(-1) at 16 days of culture, equivalent to a rate of 2.71 mg.L(-1).day(-1). To our knowledge, the paclitaxel productivity obtained in this study is one of the highest reported so far by academic laboratories for Taxus species cultures in bioreactors.

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“…Cells adhered to the well surface above the medium level, which ac- counts for the drop in viable cell density between day 0 and day 4 in all experiments. In previous work, the formation of a cell growth ring has been observed in glass shake flasks and bioreactor cultures to reduce the viable cell density and specific growth rate of Taxus cell suspension cultures (Pestchanker et al 1996). An increase in seeding density (5 Â 10 5 cells/ml) resulted in an increase in total cell number and viability was maintained (>90%) through days 10-14.…”

Section: Growth Of Aggregated Taxus Cell Suspensions In the Presence mentioning

confidence: 97%

Culture of Isolated Single Cells from Taxus Suspensions for the Propagation of Superior Cell Populations

Naill

Roberts

2005

Biotechnol Lett

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Single cells isolated from aggregated Taxus cuspidata cultures via enzymatic digestion were grown in suspension culture. High seeding density (4 x 10(5 )cells/ml) and the addition of cell-free conditioned medium were essential for growth. Doubling the concentration of the nutrients [ascorbic acid (150 g/l), glutamine (6.25 mM: ), and citric acid (150 g/l)] had no effect on single cell growth or viability. A specific growth rate of 0.11 days(-1) was achieved, which is similar to the observed growth rate of aggregated Taxus suspensions. The biocide, Plant Preservative Mixture, added at 0.2% (v/v) to all single cell cultures to prevent microbial contamination, had no significant effect on growth or viability. Following cell sorting, single cell cultures can be used to establish new cell lines for biotechnology applications or provide cells for further study.

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Kinetics of taxol production and nutrient use in suspension cultures of Taxus cuspidata in shake flasks and a Wilson-type bioreactor

Cited by 45 publications

References 14 publications

Taxane Production in Suspension Culture of Taxus × Media var. Hicksii Carried Out in Flasks and Bioreactor

Taxane Production in Suspension Culture of Taxus × Media var. Hicksii Carried Out in Flasks and Bioreactor

Effects of immobilization by entrapment in alginate and scale‐up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata

Culture of Isolated Single Cells from Taxus Suspensions for the Propagation of Superior Cell Populations

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