Taxane Production in Suspension Culture of Taxus × Media var. Hicksii Carried Out in Flasks and Bioreactor

Abstract

Paclitaxel and 10-deacetylbaccatin III (10-DAB III) were produced in suspension cultures of Taxus x media var. Hicksii grown in shake-flasks and in a 7-l bioreactor reaching, in the bioreactor, 4.4 mg l(-1) (on day 14) and 37.5 mg l(-1) (on day 11). In shake-flasks the highest total content of paclitaxel and 10-DAB III was 7.3 mg l(-1) (on day 4) and 8.8 mg l(-1) (on day 18). Phenylalanine, at 0.05 mM, increased paclitaxel accumulation in cells cultivated in bioreactor and flasks 30-fold and 9-fold (from 0.02 mg l(-1) to 0.6 mg l(-1) and to 0.2 mg l(-1), respectively). The 10-DAB III content in cells from flasks was increased from 0.4 mg l(-1) to 1.6 mg l(-1).

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

“…In this study also, elicitors have not been introduced; hence the result can be considered satisfactory in comparison with the findings obtained in available published researches. The decrease in Taxol concentration after a certain phase in this study is consistent with findings obtained by Syklowska- Baranek and Furmanowa (2005), who gave precipitation of metabolites in the medium after long exposure to be the tentative reason.…”