Kinetics of superoxide production by stimulated neutrophils

Black, Christopher D. V.; Cook, John A.; Krishna, C. Murali; Kaufman, Dwight; Russo, Angelo; Samuni, Amram; Malech, Harry L.

doi:10.1016/0003-9861(91)90017-d

Cited by 18 publications

(7 citation statements)

References 33 publications

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“…In the present study we also demonstrate that spin trapping with DEPMPO is far more sensitive than cytochrome c for measurement of • O 2 Ϫ generation from PMNs. In general, as reported in the literature, for the ferricytochrome c assay 100,000 cells are required for each measurement (5,11,47). We observe that with DEPMPO spin trapping…”

Section: Discussionmentioning

confidence: 75%

Quantitative Measurement of Superoxide Generation and Oxygen Consumption from Leukocytes Using Electron Paramagnetic Resonance Spectroscopy

Roubaud¹,

Sankarapandi²,

Kuppusamy³

et al. 1998

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 75%

Quantitative Measurement of Superoxide Generation and Oxygen Consumption from Leukocytes Using Electron Paramagnetic Resonance Spectroscopy

Roubaud¹,

Sankarapandi²,

Kuppusamy³

et al. 1998

Analytical Biochemistry

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“…Our luminol-enhanced chemiluminescence assay reflects mainly components of the myeloperoxidase-H 2 O 2 -halogen system and especially the production of hypochlorous acid (HOCl), which is crucial for neutrophil-mediated extracellular oxidation [31,32]. The contribution of cells other than neutrophils can be neglected in this assay [33].…”

Section: Oxidative Mechanismsmentioning

confidence: 99%

Oxidative autoaggression by phagocytes in human peritonitis

Billing

Jochum²,

Fröhlich

et al. 1997

Eur J Clin Investigation

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In 60 abdominal exudates from patients with the diagnosis of either acute or persistent (chronic) peritonitis, indicators of phagocyte-derived oxidative systems (myeloperoxidase, chemiluminescence) and proteases (elastase) were measured. These exudates reveal a picture of maximal inflammatory activation. Both types of exudates (30 each) showed a significant influx of inflammatory cells, with the mean leucocyte count being 73,000 microL and 32,000 microL-1 respectively. Local myeloperoxidase concentrations were approximately 1000-fold greater than that of normal plasma. Spontaneous and elicitable chemiluminescence--indicators of phagocyte respiratory burst activity--were dramatically increased. In addition, levels of extracellularly released elastase (from neutrophils) were found to be up to about 1000-fold that of normal plasma values. Although most of the elastase detected in the exudates was complexed with alpha 1-proteinase inhibitor (alpha 1 PI), enzymatically active elastase could be measured in approximately 60% of the samples being investigated. As there was an excess of immunoreactive alpha 1 PI in these exudates, the free elastase activity implies that much of the alpha 1 PI was inactive, presumably subjected to oxidative destruction. Moreover, a trypsin-inhibitory activity to antigen ratio below 1 (mean = 0.81) in 75% of the purulent exudates indicated also partial proteolytic degradation of alpha 1 PI. In contrast, 16 clear exudates (no bacteria, white cell count below 500 microL-1) taken from the non-infected peritoneal cavity of patients undergoing intra-abdominal surgery revealed a similar permeability increase of the peritoneum but did not show relevant oxidative and proteolytic activity or destruction of alpha 1 PI compared with purulent specimens. Thus, only the inflammatory process of peritonitis appears to result in an overwhelming local phagocytic activity that initiates and maintains protease inhibitor consumption and/or inactivation. The tremendous oxidative potential found in purulent exudates may cause destruction in a wide variety of defence systems.

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“…2A, multiple-lines of EPR spectra of human PMNs primed by PMA were detected. The hyperfine coupling constants (hfcc) of a N = 1.48 and a H = 1.48 mT (closed circle) at the four-line spectrum were typical DMPO spin adducts of ·OH (DMPO-OH) [22, 23], and those of a N = 1.425, a H = 1.145 and a H = 0.124 (open circle) were DMPO spin aducts of O 2 ·− (DMPO-OOH) [21, 22]. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…PMNs were prepared from the blood by density gradient centrifugation using a Ficoll-Paque Plus (d = 1.077) following dextran sedimentation as previously described [22]. After the pellet of PMNs were subjected to rapid hypotonic lysis of erythrocyte, the cells were suspended in Hank’s balanced salt solution (HBSS) to be 1.0 × 10 6 cell/ml.…”

Section: Methodsmentioning

confidence: 99%

Myristic Acid, A Side Chain of Phorbol Myristate Acetate (PMA), Can Activate Human Polymorphonuclear Leukocytes to Produce Oxygen Radicals More Potently than PMA

Tada

Ichiishi

Saito

et al. 2009

J. Clin. Biochem. Nutr.

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Myristic acid (MyA), which is a saturated fatty acid (C14:0) and a side chain of phorbol 12-myristate 13-acetate (PMA), was examined if MyA stimulates human polymorphonuclear leukocytes (PMNs) to release oxygen radicals comparable to PMA by applying electron paramagnetic resonance (EPR)-spin-trapping method. When MyA was added to isolated human PMNs, spin adducts of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)-OH and DMPO-OOH were time-dependently observed. The amounts of these spin adducts were larger than those of PMNs stimulated by PMA. These results clearly show that MyA is more potent agent to prime human PMNs than PMA, in a point of view of not only O2·− but also ·OH production. This fact calls attention that too much intake of MyA that is known to be contained vegetable oils can lead to crippling effect through uncontrolled production of reactive oxygen species.

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Kinetics of superoxide production by stimulated neutrophils

Cited by 18 publications

References 33 publications

Quantitative Measurement of Superoxide Generation and Oxygen Consumption from Leukocytes Using Electron Paramagnetic Resonance Spectroscopy

Quantitative Measurement of Superoxide Generation and Oxygen Consumption from Leukocytes Using Electron Paramagnetic Resonance Spectroscopy

Oxidative autoaggression by phagocytes in human peritonitis

Myristic Acid, A Side Chain of Phorbol Myristate Acetate (PMA), Can Activate Human Polymorphonuclear Leukocytes to Produce Oxygen Radicals More Potently than PMA

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