2014
DOI: 10.4067/s0718-58392014000100015
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Kinetics of soil enzyme activities under different ecosystems: An index of soil quality

Abstract: Soil microbial activity plays an important role in regulating biotransformation, nutrient cycling and hence the microbiological processes are at the center of many ecological functions. The kinetic parameters (Vmax and Km Michaelis constant) of different enzymes (amylase, invertase, protease, urease, and dehydrogenase) were determined in order to assess the metabolic response of soil. The maximum reaction velocity (Vmax) represents a maximum rate of activity when all enzymes are saturated, which markedly incre… Show more

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Cited by 36 publications
(16 citation statements)
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“…The variances in kinetic parameters of urease occur as a result of an increase in V max , K M , and V max /K M due to variations in physicochemical properties of the soil (Dick et al, 1994), agricultural practices such as fertilizer and pesticide use (Gianfreda et al, 1994), and synthesis of urease enzyme by increasing microbial population. Urease activity showed a positive correlation with N availability, which indicated that this enzyme can be used to make some inferences about the nitrification process in soil and determine if N losses are due to volatilization, nitrification, or denitrification (Kujur and Kumar Patel, 2014). Urease has been widely used for soil quality assessment, since its activity increases with organic fertilization and decreases with synthetic pesticide application on soil (Gianfreda et al, 1994).…”
Section: Resultsmentioning
confidence: 99%
“…The variances in kinetic parameters of urease occur as a result of an increase in V max , K M , and V max /K M due to variations in physicochemical properties of the soil (Dick et al, 1994), agricultural practices such as fertilizer and pesticide use (Gianfreda et al, 1994), and synthesis of urease enzyme by increasing microbial population. Urease activity showed a positive correlation with N availability, which indicated that this enzyme can be used to make some inferences about the nitrification process in soil and determine if N losses are due to volatilization, nitrification, or denitrification (Kujur and Kumar Patel, 2014). Urease has been widely used for soil quality assessment, since its activity increases with organic fertilization and decreases with synthetic pesticide application on soil (Gianfreda et al, 1994).…”
Section: Resultsmentioning
confidence: 99%
“…Soil enzymes, mainly produced by microorganisms, respond to the changes of soil environment rapidly (Kandeler et al, 1999) and are widely used as an indicator of specific biochemical reactions in soil (Kujur and Patel, 2014;Dick et al, 1994). Phenol oxidase, peroxidase and cellulase are indicators of complex compound metabolism.…”
Section: Roles Of Microbes Soil Enzyme and Soil Substratementioning
confidence: 99%
“…3c). Soil urease is an important regulator of the transformation of organic nitrogen to inorganic nitrogen [24][25][26] . Therefore, increases in urease activity would improve the mineralization of organic nitrogen, and consequently increase the content of soil inorganic nitrogen.…”
Section: Discussionmentioning
confidence: 99%