Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer

Su, Xiao-hang; Lei, Qun-Li; Ren, Cheng

doi:10.1088/1674-1056/24/11/113601

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…Cells can transmit substances, energy and information across their phospholipid bilayer with the surrounding environment, enabling life activities to be coordinated. [1,2] External stimuli (pH value, [3][4][5] temperature, and osmotic pressure, [6] etc.) cause phospholipid redistribution in the membrane and reassembly in structure, which affects and controls the process of cell function.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Zhou¹,

Wang²,

Ma³

et al. 2022

Chinese Phys. B

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Osmotic pressure can break the fluid balance between intracellular and extracellular solutions. In hypo-osmotic solution, water molecules, which transfer into the cell and burst, are driven by the concentrations difference of solute across the semi-permeable membrane. The complicated dynamic processes of the intermittent burst have been previously observed. However, the underlying physical mechanism has yet to be thoroughly explored and analyzed. Here, the intermittent release of inclusion in giant unilamellar vesicles was investigated quantitatively, applying the combination of experimental and theoretical methods in the hypo-osmotic medium. Experimentally, we adopted highly sensitive EMCCD to acquire intermittent dynamic images. Notably, the component of the vesicle phospholipids affected the stretch velocity, and the prepared solution of the vesicle adjusted the release time. Theoretically, we chose equations numerical simulations to quantify the dynamic process in phases and explored the influence of physical parameters such as bilayer permeability and solution viscosity on the process. It was concluded that the time taken to achieve the balance of giant unilamellar vesicles was highly dependent on the structure of the lipid molecular. The pore lifetime was strongly related with the internal solution environment of giant unilamellar vesicles. The vesicle prepared in viscous solution accessed visualized long-lived pore. Furthermore, the line tension was measured quantitatively by the release velocity of inclusion, which was in the same order of magnitude as the theoretical simulation. In all, the experimental values well matched the theoretical values. Our investigation clarified the physical regulatory mechanism of intermittent pore formation and inclusion release, which had an important reference for the development of novel technologies such as gene therapy based on transmembrane transport as well as controlled drug delivery based on liposomes.

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Section: Introductionmentioning

confidence: 99%

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Zhou¹,

Wang²,

Ma³

et al. 2022

Chinese Phys. B

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“…[63][64][65][66][67][68] All-atom molecular dynamics and Monte Carlo (MC) simulations are widely used as important methods to complement theories and experiments in simulating charged systems and make predictions that agree well with experiments in a wide range of ion conditions. [23,[69][70][71][72][73][74][75][76] Since multivalent ions are so important to nucleic acid structures and PB theory is concise and has efficient solving algorithms, [40,41,66] it is interesting to understand how much PB predictions deviate for multivalent ion distributions around nucleic acids and to quantify the extent to which ionic neutralization, multivalent ion distributions around nucleic acids can be approximately described by PB theory.…”

Section: Introductionmentioning

confidence: 99%

To what extent of ion neutralization can multivalent ion distributions around RNA-like macroions be described by Poisson–Boltzmann theory?

Xiong¹,

Kun²,

Zhang³

et al. 2018

Chinese Phys. B

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Nucleic acids are negatively charged biomolecules, and metal ions in solutions are important to their folding structures and thermodynamics, especially multivalent ions. However, it has been suggested that the binding of multivalent ions to nucleic acids cannot be quantitatively described by the well-established Poisson-Boltzmann (PB) theory. In this work, we made extensive calculations of ion distributions around various RNA-like macroions in divalent and trivalent salt solutions by PB theory and Monte Carlo (MC) simulations. Our calculations show that PB theory appears to underestimate multivalent ion distributions around RNA-like macroions while can reliably predict monovalent ion distributions. Our extensive comparisons between PB theory and MC simulations indicate that when an RNA-like macroion gets ion neutralization beyond a "critical" value, the multivalent ion distribution around that macroion can be approximately described by PB theory. Furthermore, an empirical formula was obtained to approximately quantify the critical ion neutralization for various RNAlike macroions in multivalent salt solutions, and this empirical formula was shown to work well for various real nucleic acids including RNAs and DNAs.

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“…[1,2] Phospholipids, mostly liquid crystal and the most abundant lipids in cell membranes, [3,4] are used in a wide range of applications for the detection and characterization of biomolecular interactions, including biological sensing, transmembrane transport, receptor interactions, and cellular signaling. [5][6][7] Lipids and proteins are the basis of the biological membrane structure and the interaction between them has been widely studied, [8][9][10][11][12] which is one of the basic problems of modern biochemistry and biophysics. For instance, the signal transduction process via the cell membrane is controlled by membrane-associated [13] and soluble proteins.…”

Section: Introductionmentioning

confidence: 99%

Behavior of lysozyme adsorbed onto biological liquid crystal lipid monolayer at the air/water interface

et al. 2016

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The interaction between proteins and lipids is one of the basic problems of modern biochemistry and biophysics. The purpose of this study is to compare the penetration degree of lysozyme into 1,2-diapalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethano-lamine (DPPE) by analyzing the data of surface pressure-area (π-A) isotherms and surface pressure-time (π-T ) curves. Lysozyme can penetrate into both DPPC and DPPE monolayers because of the increase of surface pressure at an initial pressure of 15 mN/m. However, the changes of DPPE are larger than DPPC, indicating stronger interaction of lysozyme with DPPE than DPPC. The reason may be due to the different head groups and phase state of DPPC and DPPE monolayers at the surface pressure of 15 mN/m. Atomic force microscopy reveals that lysozyme was absorbed by DPPC and DPPE monolayers, which leads to self-aggregation and self-assembly, forming irregular multimers and conical multimeric. Through analysis, we think that the process of polymer formation is similar to the aggregation mechanism of amyloid fibers.

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Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer

Cited by 5 publications

References 35 publications

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

To what extent of ion neutralization can multivalent ion distributions around RNA-like macroions be described by Poisson–Boltzmann theory?

Behavior of lysozyme adsorbed onto biological liquid crystal lipid monolayer at the air/water interface

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