Behavior of lysozyme adsorbed onto biological liquid crystal lipid monolayer at the air/water interface

Abstract

The interaction between proteins and lipids is one of the basic problems of modern biochemistry and biophysics. The purpose of this study is to compare the penetration degree of lysozyme into 1,2-diapalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethano-lamine (DPPE) by analyzing the data of surface pressure-area (π-A) isotherms and surface pressure-time (π-T ) curves. Lysozyme can penetrate into both DPPC and DPPE monolayers because of the increase of surface pressure at an initial pressure of 15 mN/m. However, the changes of DPPE are larger than DPPC, indicating stronger interaction of lysozyme with DPPE than DPPC. The reason may be due to the different head groups and phase state of DPPC and DPPE monolayers at the surface pressure of 15 mN/m. Atomic force microscopy reveals that lysozyme was absorbed by DPPC and DPPE monolayers, which leads to self-aggregation and self-assembly, forming irregular multimers and conical multimeric. Through analysis, we think that the process of polymer formation is similar to the aggregation mechanism of amyloid fibers.

A Lipidated Peptide with Mitochondrial Membrane Localization in Human A549 Lung Cells: From Enhanced Cell-Penetrating Properties to Biological Activity Mechanism

“…The time dependence in Figure 2 indicates the course of adsorption, binding, insertion, and rearrangement of new pHK-pKV molecules at the interface. 44 Figure 2 (red plot) shows a smooth increase of the surface pressure with time, which characterizes the interaction between the amphiphilic peptide Pal-pHK-pKV and the DPPG monolayer. Pal-pHK-pKV is a more hydrophobic peptide, as compared to pHK-pKV, due to the conjugated palmitic (Pal) alkyl chain.…”

“…Thus, pHK-pKV and DPPG phospholipid form mixed interfacial membrane assemblies, for which the surface pressure gradually reaches a stable equilibrium state. The time dependence in Figure indicates the course of adsorption, binding, insertion, and rearrangement of new pHK-pKV molecules at the interface …”

Layering of confined water between two graphene sheets and its liquid–liquid transition

“…Water, the fountainhead of the cosmic inventory, is not only so indispensable to all living creatures that it renders its involvement in a vast number of processes which has important relevance to the survival of every organism such as respiration and the transportation of nutrients and metabolite, but also a topic perpetual interest due to its remarkable anomalous properties and important implications to natural science, [1] biological science, [2][3][4][5][6] material science, [7][8][9][10][11] etc. The bulk water has various solid structures including more than 15 crystalline phases and 3 amorphous phases.…”