Kinetics of Plasma Hemoglobin Catabolism in the Rat. II. Cr51-Tagged Hemoglobin.

The Renal Handling of Hemoglobin

“…Dialysis effectively removed all unbound 5ZCr from the hemoglobin solution. This was demonstrated on several occasions by chromatographing an aliquot of the dialyzed 51Cr hemogiobin on Sephadex G-25, which effectively separates the protein-bound and unbound SlCr (8). The specific activities of the hemogiobin sohitions before and after chromatography were always identical.…”

“…others have shown rat hemoglobin is more soluble in alkaline solution (8). Each hemolysate was then centrifuged at 4°C for 30 rain at 5000 g. The crystal dear supernatant was dialyzed overnight at 4°C against a mixture comprised of three parts normal saline and one part isotonic phosphate buffer, pH 7.4 (buffered saline).…”

Haptoglobins In Some Galliformes Birds

“…The clearance rate in man is zero order kinetics (Lathem and Worley, 1959), but Faulstick et al (1962) noted a change to first order kinetics when the level of Hb administered was lower than the haemoglobin binding capacity. In rats (Gabrielli et al, 1963) dual kinetics has also been observed.…”

“…In birds, the clearance rate of Hb-59 Fe is exponential as has been observed in rabbits (Murray et al, 1961) and in rats (Franklin et al, I960;Gabrielli et al, 1962Gabrielli et al, , 1963. The existence of two different rates could be explained by the elimination of free Hb and Hb-Hp complex, using first order and zero order kinetics, respectively.…”