Kinetics of Petroleum-Contaminated Soil Biodegraded by An Indigenous Bacteria Bacillus megaterium

Yudono, Bambang; Said, Muhammad; Sabaruddin, Sabaruddin; Napoleon, Adipati; Utami, Maryati Budi

doi:10.4308/hjb.17.4.155

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…In the current work, the authors have successfully isolated the PS-degrading strain and identified as Bacillus megaterium strain based on 16S rRNA sequencing aligned with Gen Bank database using Basic Local Alignment Search Tool (BLAST) and the data is in the process of publication. With reference to previous literatures, it has been reported Bacillus megaterium strain which inhabited in the oil contaminated sites was believed to be capable to metabolize both n-alkanes and aromatic hydrocarbons in petroleum [4,5]. The ability of Bacillus megaterium in degrading petroleum has been widely applied in solving environmental problems such as biodegradation of oily sludge and crude oil removal [4,6,7], diesel degradation [8] and bioremediation of lubricant oil pollution in water [9].…”

Section: Introductionmentioning

confidence: 99%

Bacillus megaterium: a Potential and an Efficient Bio-Degrader of Polystyrene

Tan

Kassim

Razak

et al. 2021

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 99%

Bacillus megaterium: a Potential and an Efficient Bio-Degrader of Polystyrene

Tan

Kassim

Razak

et al. 2021

Braz. arch. biol. technol.

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“…With reference to previous literature on the biodegradation of aromatic compounds, Bacillus megaterium strain in the oil-contaminated sites was believed to metabolise both n-alkanes and aromatic hydrocarbons in petroleum [23,24]. It has also been reported that Bacillus megaterium is a lignin peroxidase producing bacteria which indicated its potential in lignin degradation [25].…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Polystyrene Degrading Bacteria from Zophobas morio’s Gut

Tan

Fauzi

Kassim

et al. 2021

Walailak J Sci & Tech

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Polystyrene (PS) and expanded polystyrene (EPS) are commodity plastics widely used in many industries. The increase of PS in the environment causes ecological threats. It has been observed that the beetle larvae feed on plastic packages, and this has drawn the researcher’s attention towards the complex system within the larvae’s gut where relationships between fungi, bacteria, and the insect host have been established. The objective of this study is to isolate and identify PS-degrading microbe from super worms. The study used polystyrene as a sole carbon source to isolate the positive PS-degrading bacteria. Five bacterial colonies which showed positive growth on the modified PS-agar plate were isolated. All five colonies of the PS-degrading bacterial isolates were identified using partial 16 S rRNA gene sequencing. The results showed a close relationship between Bacillus aryabhattai and Bacillus megaterium strains. An examination via biochemical studies for distinction was implemented further. Based on the morphological characterization and taxonomic method, the isolates were identified as Bacillus megaterium. The isolates' viability was tested using 2,3,5-triphenyltetrazolium chloride (TTC), and positive results were obtained with red-colored insoluble triphenylformazan (TPF) formed within 5 to 7 days. The isolation and identification of these bacteria from Zophobas morio’s gut may provide a promising source of a plastic degrading enzyme that can be further studied and manipulated in developing enzymatic biodegradation in plastic waste management.

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