Kinetics of neovascularisation of splenic autotransplants in mice

Alves, Humberto José; Viana, Gisele; Magalhães, Mônica Moreira de; Arantes, Rosa Maria Esteves; Coelho, Paulo Marcos Zech; Cunha-Melo, José Renan

doi:10.1046/j.1469-7580.1999.19530387.x

Cited by 4 publications

(3 citation statements)

References 15 publications

(25 reference statements)

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“…Several animal model studies have assessed the effect of splenic autotransplantation on sepsis after splenectomy. Alves et al reported that after splenic autotransplantation in mice, there is neovascularization between the transplanted spleen and the main vessels on the third day after transplantation; the blood supply of the splenic fragments is eventually provided in a centripetal manner by the splenic, short gastric, mesenteric, and gastroepiploic arteries [ 24 ]. Patel et al showed that, in splenectomized rabbits, autotransplantation with small slices of spleen in the peritoneal cavity improves the clearance of pneumococci from the blood [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

A solution to the negative effects of splenectomy during colorectal trauma and surgery: an experimental study on splenic autotransplantation to the groin area

et al. 2015

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BackgroundSplenectomy after combined colosplenic trauma or iatrogenic splenic injury during colorectal surgery associates with worse short- and long-term outcomes, including reduced survival in patients with colorectal cancer. Splenic autotransplantation may improve the outcomes of such patients. Omental splenic transplantation is the standard procedure but may be difficult when performing laparoscopic colorectal surgery or when total or subtotal omentectomy is required. This animal model study was performed to evaluate the impact of splenic autotransplantation to the groin area on colonic wound healing.MethodsThirty rats were divided into three groups of ten animals. One group underwent colon anastomosis and sham splenectomy, the second underwent colon anastomosis and splenectomy, and the third underwent colon anastomosis, splenectomy, and intramuscular autotransplantation of the spleen. On postoperative day 7, anastomotic healing was evaluated by measuring bursting pressure and hydroxyproline levels. The third group was subjected to scintigraphy before sacrifice to assess whether the transplant was functional.ResultsThe mortality rates of the sham, splenectomized, and transplanted animals were 0 %, 30 %, and 20 %, respectively: the splenectomized animals had significantly lower mean bursting pressures than the other two groups (p = 0.002). The mean hydroxyproline levels of the three groups were 467.4, 335.3, and 412.7 mg hydroxyproline/g protein, respectively (p = 0.0856). Nine of the ten transplanted animals (90 %) had splenic activity on scintigraphy.ConclusionsSplenectomy impaired the healing of the colonic anastomosis. This effect was largely reversed by splenic autotransplantation. Intramuscular autotransplantation to the groin area appears to be feasible and effective.

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Section: Discussionmentioning

confidence: 99%

A solution to the negative effects of splenectomy during colorectal trauma and surgery: an experimental study on splenic autotransplantation to the groin area

et al. 2015

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“…These observations are corroborated by more recent studies of neovasculogenesis in murine transplants, with precise measurements enabled by injection of tracers (fluorescent polystyrene microspheres) and electron microscopy. Although the new vessels at the periphery of the grafts started to form on day 3 p/t, microcirculation within the marginal zone of white pulp remained rudimentary until week 10 p/t [ 48 ].…”

Section: Autotransplantations Of the Spleenmentioning

confidence: 99%

Spleen: Reparative Regeneration and Influence on Liver

Elchaninov

Vishnyakova

Сухих

et al. 2022

Life

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This review considers experimental findings on splenic repair, obtained in two types of small animal (mouse, rat, and rabbit) models: splenic resections and autologous transplantations of splenic tissue. Resection experiments indicate that the spleen is able to regenerate, though not necessarily to the initial volume. The recovery lasts one month and preserves the architecture, albeit with an increase in the relative volume of lymphoid follicles. The renovated tissues, however, exhibit skewed functional profiles; notably, the decreased production of antibodies and the low cytotoxic activity of T cells, consistent with the decline of T-dependent zones and prolonged reduction in T cell numbers. Species–specific differences are evident as well, with the post-repair organ mass deficiency most pronounced in rabbit models. Autotransplantations of splenic material are of particular clinical interest, as the procedure can possibly mitigate the development of post-splenectomy syndrome. Under these conditions, regeneration lasts 1–2 months, depending on the species. The transplants effectively destroy senescent erythrocytes, assist in microbial clearance, and produce antibodies, thus averting sepsis and bacterial pneumonia. Meanwhile, cellular sources of splenic recovery in such models remain obscure, as well as the time required for T and B cell number reconstitution.

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“…17 In mice, such grafts present centripetal revascularization after the third day following operation. 18 The most efficient function of these grafts however occurs after the 16th week. 19 Subtotal splenectomy with the preservation of the organ's inferior pole (STPI), has been described in dogs 7 and in rats.…”

Section: Introductionmentioning

confidence: 99%

Preservative spleen surgery and hyperbaric oxygen therapy

Paulo

Cintra³

et al. 2007

Acta Cir. Bras.

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Purpose :To assess functional and morphological aspects of spleen auto-implants and of the splenic inferior pole of rats, post-operatively treated or not with hyperbaric oxygen, as well as the survival of these animals, were studied. Methods: Seventy-eight male Wistar rats, weighing between 192 and 283 g ( 238,3 ± 9,6g), were randomly distributed into three groups: Group1-(n=20), spleen manipulation; group 2-(n=36), spleen auto-implantation; group3-(n= 22), subtotal splenectomy preserving the inferior pole. Each group was subdivided as follows: subgroup a, not submitted to hyperbaric oxygen therapy: 1a(n=10), 2a(n=21), 3a(n= 13); subgroup b, submitted to the therapy: 1b(n=10), 2b(n=15), 3b(n=9). Blood was collected pre-operatively and 11 days after surgery, for the estimation of lipids and immunoglobulins and the counting of platelets and Howell-Jolly corpuscles. The spleen and remains were taken for histological study. Results : The number of surviving animals was significantly higher in groups 1(p<0,01) and 3(p<0,05) relative to those of subgroup 2a. Total cholesterol and the LDL fraction increased significantly in subgroup 2a (p<0,01) and 3a (p<0,05), and remained unaltered in subgroups 2b e 3b. IgM decreased more significantly in subgroup 2 than in subgroup 3 (p<0,001 vs p<0,01). The increase of platelet numbers and the appearance of Howell Jolly corpuscles was smaller in subgroup 2b compared to subgroup 2a , and in group 3 compared to group aqui-> 2. The macro and microscopic appearance in subgroup 2b were more viable than in subgroup 2a, and that of group 3 more viable than in group 2. The survival of the animals carrying their whole spleen or its inferior pole was more frequent than that of the auto-implanted animals. Conclusion : Functionality and viability of the whole spleen or of its inferior pole, were better than in the auto-implanted animals. Hyperbaric oxygentherapy contributed to increased survival frequency of auto -implanted animals, and to improve the functionality and viability of the auto-implants and the function of the inferior splenic pole, and did not interfere in animals carrying their whole spleen. Key words: Splenectomy/methods.Spleen/metabolism. Adverse effects/splenectomy. Oxygen inhalation therapy/methods RESUMO Objetivo : Estudar aspectos funcionais e morfológicos dos auto-implantes esplênicos e do pólo inferior do baço de ratos, tratados ou não com oxigênio hiperbárico no pós-operatório, e a sobrevida desses animais. Métodos: Foram operados 78 ratos, machos, Wistar, pesando entre 192 g e 283 g ( M.A 238,3 ± 9,6) , distribuídos aleatoriamente em três grupos : 1-(n=20) , manipulação do baço; 2-(n=36), auto-implante esplênico; 3(n= 22), esplenectomia subtotal com preservação do pólo inferior. Cada grupo foi dividido em dois subgrupos: a-não submetido à oxigenoterapia hiperbárica: 1a(n=10), 2a(n=21), 3a(n= 13); b-submetido: 1b(n=10), 2b(n=15), 3b(n=9). O sangue foi colhido para dosagem dos lípides e imunoglobulinas e contagem das plaquetas e dos corpúsculos de Howell-Jolly n...

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Kinetics of neovascularisation of splenic autotransplants in mice

Cited by 4 publications

References 15 publications

A solution to the negative effects of splenectomy during colorectal trauma and surgery: an experimental study on splenic autotransplantation to the groin area

A solution to the negative effects of splenectomy during colorectal trauma and surgery: an experimental study on splenic autotransplantation to the groin area

Spleen: Reparative Regeneration and Influence on Liver

Preservative spleen surgery and hyperbaric oxygen therapy

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