Kinetics of Monofluorophosphate Hydrolysis in a Bacterial Test Plaque in situ

Tenuta, Lívia Maria Andaló; Cury, Altair Antoninha Del Bel; Tabchoury, Cínthia Pereira Machado; Moi, Gisele Pedroso; Silva, Wander José da; Cury, Jaime Aparecido

doi:10.1159/000279466

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Based on the comparison with the Na2FPO3 pure solution prepared in the laboratory as control, low reactivity was not due to pH or interferents of this commercial mouth rinse (Table 2), as commercial and control did not show statistical differences (p>0.05). Mouth rinses formulated with Na2FPO3 could be effective on caries control if the mechanism of action of fluoride from these commercial products is not their reactivity, but the simple diffusion to dental biofilms, where MFPases hydrolyze Na2FPO3 and fluoride ion is released to interfere with the caries process (32).…”

Section: Discussionmentioning

confidence: 99%

Fluoride bioavailability on demineralized enamel by commercial mouth rinses

et al. 2021

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The ability of mouth rinses, available in the international market, to form reaction products on demineralized enamel (bioavailability test) was evaluated in vitro. Nine mouth rinses purchased in Chile were evaluated; eight formulated with NaF (one containing 100 µg F/mL and seven containing 226) and one with Na2FPO3 (226 µg F/mL as ion F). Demineralized enamel slabs (n=15 per mouth rinse) were sectioned; one half was subjected to the assigned mouth rinse treatment for 10 min and the other half was used to obtain baseline data. Loosely bound and firmly bound fluoride formed on enamel were determined with an ion-specific electrode and the values were expressed in µg F/cm2. The concentration of fluoride and the pH of the mouth rinses were previously determined. Concentrations of loosely bound and firmly bound fluoride formed on enamel were independently analyzed by ANOVA and Tukey’s test (α=5%). The loosely bound and firmly bound fluoride concentrations (µg F/cm2) formed ranged from 3.2 to 36.2 and 0.4 to 1.7, respectively. Loosely bound fluoride formed on enamel was significantly more effective in discriminating the effect of different commercial mouth rinses than firmly bound fluoride. Mouth rinses with 226 ppm F as NaF and low pH presented significantly greater bioavailability of fluoride on enamel than those with higher pH or lower NaF concentration. The mouth rinse with Na2FPO3 showed low reactivity. Although further studies are necessary, the findings showed that commercial fluoride-containing mouth rinses have important variations in enamel fluoride bioavailability, which may result in differences on anticaries efficacy.

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Section: Discussionmentioning

confidence: 99%

Fluoride bioavailability on demineralized enamel by commercial mouth rinses

et al. 2021

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“…1984 [124] Enamel de-and remineralization tested in an in situ model using sound and demineralized slabs mounted in troughs in lower removable appliances with channels for salivary access Creanor et al, 1986 [127] Enamel lesions mounted in partial dentures to study the remineralization and F uptake during use of toothpastes containing different F concentrations de Kloet et al, 1986 [128] Thin sections of enamel with artificial caries lesions mounted in partial dentures to assess remineralization by F toothpastes Mellberg at al., 1986 [129] Remineralizing potentials of conventional and high-F toothpastes studied in situ using matched pairs of enamel lesions Schafer, 1989 [131] Sound or artificially demineralized enamel slabs mounted 1-mm recessed in a removable palatal appliance, covered by a plastic mesh to favor biofilm accumulation; F toothpaste containing baking soda tested against a F toothpaste, with no additional effect Cury et al, 2001 [142] Inhibition of enamel demineralization by F toothpaste tested in situ under increasing frequencies of exposure to carbohydrates Duggal et al, 2001 [143] Association between F-releasing restorative materials and F toothpastes tested in situ; the effect of regular F toothpaste use on resting biofilm fluid fluoride determined Cenci at al., 2008 [150] The remineralizing potential of a low F acidic toothpaste tested in situ Nobre dos Santos et al, 2007 [148] A calcium pre-rinse before F toothpaste use tested in situ Magalhães et at., 2007 [149] Association between APF application and F toothpaste use on enamel caries tested in situ Paes Leme et al, 2004 [116] Inhibition of deciduous enamel demineralization under exposure to toothpastes with different F concentrations, and increasing cariogenic chellenges tested in situ Cury et al, 2010 [77] Hydrolysis of MFP from toothpaste studied using the short-term in situ model Tenuta et al, 2010 [152] Brushing time and toothpaste amount tested in a short-term in situ model Zero et al, 2010 [73] An in situ interproximal model used to assess enamel remineralization by increasing F concentration toothpastes Dunipace et al, 1997 [137] Arrestment of root surface caries by F toothpaste tested in situ Nyvad et al, 1997 [138] Fig. 4.…”

Section: Discussionmentioning

confidence: 99%

Laboratory and Human Studies to Estimate Anticaries Efficacy of Fluoride Toothpastes

Tenuta

Cury²

2013

Monographs in Oral Science

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Much more than mechanical biofilm removal, toothbrushing with fluoride toothpastes is an effective way of increasing the availability of fluoride in the oral cavity to reduce demineralization and enhance remineralization of enamel and dentine. These effects of fluoride toothpastes have been estimated by a wide range of laboratory and human studies, which have helped to develop anticaries effective formulations and understand their mechanism of action. These studies have focused on the availability of fluoride in the toothpaste formulations, its bioavailability in saliva and remnants of disturbed biofilm, its reaction with the dental substrate to form loosely bound reservoirs as well as the ultimate reduction of mineral loss and increase in mineral and fluoride content of caries lesions. The specifics of these modes of action and their application in in vitro, in situ and in vivo preclinical tests is presented and discussed.

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“…If the anticaries mechanism of mouth rinses depends on the reactivity of fluoride with enamel-dentine surfaces, it is not expected that the Na 2 FPO 3 in this product be effective, because, similar to toothpaste, the FPO 3 -2 ion must first be hydrolyzed in the F ion. 13 The discussion on the fluoride content of mouth rinses available in the market, and the legislation that regulates their use does not apply solely to Brazil and Chile. Similarly, mouthwashes with low fluoride concentration (< 0.05% NaF) or with Na 2 FPO 3 have also been reported in Saudi Arabia and Russia.…”

Section: Discussionmentioning

confidence: 99%

Fluoride concentration in mouth rinses marketed in Chile and Brazil, and a discussion regarding their legislations

et al. 2021

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For fluoride to be effective in controlling caries, it should be bioavailable in commercial products, so that it can be released into the mouth in the products it contains. We chemically determined the available fluoride and pH in nine mouth rinses marketed in Chile, and eleven, in Brazil, and then discussed the legislation in force in both countries, regarding the anticaries potential of these oral hygiene products. The fluoride was analyzed with an ion-selective electrode (F-ISE), using the direct technique. The determinations were made in duplicate, and the results were expressed in ppm F (μg F/mL). The total fluoride concentration found in all the mouth rinses evaluated ranged from 94.7 to 233.5 ppm F, and closely matched what was declared by the manufacturers (100.0 to 226.2 ppm F). However, some mouth rinses showed lower fluoride concentrations (90 and 180 ppm F) in both countries. A Na 2 FPO 3 -formulated mouth rinse was found only in Chile, with 216.8 ppm F as the FPO 3 2ion, and 4.9 ppm F as the F -. The findings show that fluoride was potentially bioavailable in all the mouth rinses evaluated. Regarding the national legislations, although the mouthwashes sold in Brazil comply with the Brazilian legislation, discrepancies were found for Chile. However, neither country had a legislation matching the best available evidence on fluoride mouthwash efficacy for caries control. Thus, some products with low fluoride concentrations (below 226 ppm F), or manufactured with a fluoride salt other than NaF (Na 2 FPO 3 ) are being sold in the Brazilian and Chilean markets.

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Kinetics of Monofluorophosphate Hydrolysis in a Bacterial Test Plaque in situ

Cited by 7 publications

References 32 publications

Fluoride bioavailability on demineralized enamel by commercial mouth rinses

Fluoride bioavailability on demineralized enamel by commercial mouth rinses

Laboratory and Human Studies to Estimate Anticaries Efficacy of Fluoride Toothpastes

Fluoride concentration in mouth rinses marketed in Chile and Brazil, and a discussion regarding their legislations

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