Laboratory and Human Studies to Estimate Anticaries Efficacy of Fluoride Toothpastes

Tenuta, Lívia Maria Andaló; Cury, Jaime Aparecido

doi:10.1159/000350479

Cited by 65 publications

(83 citation statements)

References 128 publications

(144 reference statements)

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“…Any model has limitations to adequately simulate the in vivo conditions (19). The use of bovine enamel as a surrogate of human enamel can be considered acceptable given that similar reactivity with both of them has been observed after the use of professional fluoride products (4,6).…”

Section: Fluoride Varnish Reaction Is Time-dependentmentioning

confidence: 99%

Insoluble NaF in Duraphat® May Prolong Fluoride Reactivity of Varnish Retained on Dental Surfaces

et al. 2014

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There is no consensus about the clinical recommendation of the time that Duraphat ® varnish should be maintained on enamel surfaces without suffering mechanical disturbance by the patient. Considering the importance of calcium fluoride (CaF 2 )-like reservoirs on the anticaries effect of professional fluoride application, an in vitro study was designed to test the reactivity of Duraphat ® varnish with enamel forming these reservoirs as a function of time. Since most fluoride in Duraphat ® varnish is insoluble to react and form products on enamel, the relative contribution of the varnish soluble and insoluble fluoride fractions to the reactivity was also evaluated. For this, whole-varnish, containing soluble and insoluble fluoride (total fluoride concentration of 23699±384 μg F/g), or centrifuged varnish, containing only soluble fluoride (fluoride concentration of 258±97 μg F/g), were applied in a standardized manner on enamel slabs (n=8/varnish group/time), which were immersed in continuously renewed artificial saliva for up to 36 h. CaF 2 -like reservoirs formed on enamel by varnish application were extracted using 1 M KOH and fluoride concentration was measured with ion specific electrode. The results were expressed as µg F/cm² of enamel area. Whole varnish formed significantly higher fluoride concentration on enamel than centrifuged varnish, reaching maximum concentration at 24 h (22.0±4.5 µg F/cm²). Centrifuged varnish reached maximum concentration at 6 h (3.20±0.81 µg F/ cm²). In conclusion, a longer varnish retention time than the usually recommended could improve the anticaries effect of Duraphat ® varnish, allowing that NaF particles, initially insoluble in the varnish matrix, prolong the reactivity with enamel.

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Section: Fluoride Varnish Reaction Is Time-dependentmentioning

confidence: 99%

Insoluble NaF in Duraphat® May Prolong Fluoride Reactivity of Varnish Retained on Dental Surfaces

et al. 2014

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“…The reactivity of fluoride from dentifrices with the tooth surface is also considered as a mode of action of FD [Tenuta and Cury, 2013]. In our study, for all studied conditions, the CaF 2 and FAp concentrations varied directly according to the frequency of FD ( fig.…”

Section: Discussionmentioning

confidence: 93%

“…© 2016 S. Karger AG, Basel Fluoride dentifrice (FD) is the most rational way to use fluoride [Fejerskov et al, 2015] and the caries decline observed in developed countries after the 1980s [Bratthall et al, 1996] and in some developing countries after the 1990s [Cury et al, 2004] has been attributed to the widespread use of FD. The mechanisms by which FD controls dental caries were recently reviewed [Tenuta and Cury, 2013]. FD is able not only to reduce caries progression on sound enamel-dentine surfaces ('preventive effect'), but also able to remineralize preexisting caries lesions ('therapeutic effect').…”

mentioning

confidence: 99%

Frequency of Fluoride Dentifrice Use and Caries Lesions Inhibition and Repair

et al. 2016

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The clinical relevance of the frequency of fluoride dentifrice (FD) use on enamel caries is based on evidence. However, the relative effect of FD on reduction of demineralization or enhancement of remineralization is unknown and the effect of frequency on root dentine caries has not been explored. The aim of this double-blind, crossover, in situ study, which was conducted in 4 phases of 14 days each, was to evaluate the relationship between the frequency of FD use and enamel and root dentine de- and remineralization. Eighteen volunteers wore palatal appliances containing enamel and root dentine slabs, either sound or carious. Biofilm accumulation on the slab surface was allowed, and 20% sucrose solution was dripped 3 or 8 times per day on the carious and sound slabs, respectively. Volunteers used FD (1,100 μg F/g) in the frequencies 0 (fluoride-placebo dentifrice), 1, 2 and 3 times per day. The demineralization and remineralization that occurred in sound or carious slabs was estimated by the percentage of surface hardness loss (%SHL) or recovery (%SHR). Loosely (CaF₂) and firmly (FAp) bound fluoride concentrations were also determined. The relationship between the variables was analyzed by linear regression. The %SHL, CaF₂ and FAp concentrations were a function of the frequency of FD use for enamel and dentine, but the %SHR was a function of the frequency of FD use only for enamel (p < 0.05). The results suggest that demineralization in enamel and root dentine is reduced in proportion to the frequency of FD use, but for remineralization the effect of the frequency of FD use was relevant only to enamel.

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“…Evidence has demonstrated that modern fluoridated toothpastes, including among others either NaF-or SnF 2 -based toothpastes, are effective at controlling dental car-ies [Walsh et al, 2010] because of fluoride's activity in reducing demineralization and enhancing remineralization [ten Cate, 2013;Tenuta and Cury, 2013]. Beside the caries control effect, SnF 2 -containing toothpastes have been reported to reduce dental plaque [Sharma et al, 2013], bleeding [Gerlach and Amini, 2012], halitosis [Farrell et al, 2008], erosion [Bellamy et al, 2014] and dentin hypersensitivity [Parkinson et al, 2013].…”

mentioning

confidence: 99%

Effect of Fluoride-Containing Toothpastes on Enamel Demineralization and <b><i>Streptococcus mutans</i></b> Biofilm Architecture

et al. 2016

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This study aimed to explore the effect of fluoridated toothpastes on biofilm architecture and enamel demineralization in an in vitro biofilm model. Streptococcus mutans was grown on enamel and treated with slurries of commercial toothpastes, containing SnF₂ or NaF. Water and chlorhexidine were used as negative and positive controls, respectively. The developed biofilms were imaged and enamel demineralization was measured. SnF₂ and NaF toothpaste treatments significantly reduced enamel demineralization, but SnF₂ toothpaste was more effective. Only SnF₂ toothpaste and chlorhexidine treatments caused reductions on biofilm mass and thickness. In conclusion, this biofilm model was able to differentiate the effects of the SnF₂ and NaF toothpastes on biofilm architecture and enamel demineralization.

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Laboratory and Human Studies to Estimate Anticaries Efficacy of Fluoride Toothpastes

Cited by 65 publications

References 128 publications

Insoluble NaF in Duraphat® May Prolong Fluoride Reactivity of Varnish Retained on Dental Surfaces

Insoluble NaF in Duraphat® May Prolong Fluoride Reactivity of Varnish Retained on Dental Surfaces

Frequency of Fluoride Dentifrice Use and Caries Lesions Inhibition and Repair

Effect of Fluoride-Containing Toothpastes on Enamel Demineralization and <b><i>Streptococcus mutans</i></b> Biofilm Architecture

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