Kinetics of α₂-macroglobulin and α₁-acid glycoprotein in rats subjected to repeated acute inflammatory stimulation

Abstract

The kinetics of a 2 -macroglobulin (a2M) and a 1 -acid glycoprotein (AAG) in rats repeatedly stimulated with intramuscular injections of turpentine oil at doses 0.05 and 0.4 mL/rat were investigated. Mean serum levels of a2M peaked at 48 h after the first turpentine oil injection, reaching 1.74 and 2.36 mg/mL at 0.05 and 0.4 mL/rat, respectively. AAG peaks were also observed at 48 h after injection, and the mean values were 2.02 and 2.53 mg/mL, respectively. These peak values of a2M and AAG differed significantly between the 0.05 and 0.4 mL/rat injection groups. Mean serum levels of interleukin-6 (IL-6) at 0.05 mL/rat were 52.61 pg/mL at 12 h, 48.86 pg/mL at 36 h and 81.93 pg/mL at 84 h after the first injection. Mean IL-6 serum levels at 0.4 mL/rat were 215.24 pg/mL at 12 h, 56.33 pg/mL at 36 h and 39.25 pg/mL at 84 h after the first injection. Mean serum levels of cytokineinduced chemoattractant-1 (CINC-1) at a dose of 0.05 mL/rat were 5.70 ng/mL at 12 h, 5.58 ng/mL at 36 h and 4.58 ng/mL at 84 h after the first injection. Mean serum levels of CINC-1 after injection at 0.4 mL/rat were 11.57 ng/mL at 12 h, 4.68 ng/mL at 36 h and 4.42 ng/mL at 84 h. Serum levels of IL-6 differed significantly at 12, 24, 72 and 84 h, while those of CINC-1 differed significantly at 12, 24, 48 and 96 h between the 0.05 and 0.4 mL/rat injection groups. Differences in peak serum levels in the 0.05 and 0.4 mL/rat groups were attributed to differences in the production of IL-6 and CINC-1, which are thought to contribute to a2M and AAG production.

The Effects of Interleukin-6 and Cytokine-Induced Neutrophil Chemoattractant-1 on .ALPHA.2-Macroglobulin Production in Rats

“…Jinbo et al measured the changes of many cytokines in rats injected with turpentine oil and found that only interleukin-6 (IL-6) and cytokine-induced neutrophil chemoattractant-1 (CINC-1) increase prior to the elevation of α2M serum levels [17]. IL-6 and CINC-1 levels also increase prior to elevation of α 1 -acid glycoprotein levels in rats stimulated by either injection of turpentine oil or inoculation with Staphylococcus aureus [12]. Therefore, IL-6 and CINC-1 are presumed to contribute to the production of acute phase proteins during the inflammatory response.…”

Impairment of α₂-Macroglobulin Synthesis in Experimental Hepatopathic Rats Treated with Turpentine Oil

“…Turpentine oil is known to induce acute inflammation and has been used to induce acute inflammation in rats and dogs [1,3,6,8,9,12]. in this study, turpentine oil (Wako Pure Chemical industries, Ltd.) was thus used to induce acute inflammation by intramuscular injection at 1.0 ml/kg body weight at 2 days after the end of acetaminophen or CCl 4 administration.…”

“…Serum concentrations of α2M were measured by an enzyme-linked immunosorbent assay (eLisa) according to the procedure described by Honjo et al [8].…”

“…Introduction α 2 -Macroglobulin (α2M) and α 1 -acid glycoprotein (α 1 -aG) are typical acute-phase proteins in rats [8,9]. Serum concentrations of α2M and α 1 -aG increase by 50-to 290-fold and 34-fold, respectively, compared with pretreatment values in rats during induced acute inflammation [8].…”

“…Serum concentrations of α2M and α 1 -aG increase by 50-to 290-fold and 34-fold, respectively, compared with pretreatment values in rats during induced acute inflammation [8]. In this way, α2M responds more sensitively than α 1 -AG as a marker of acute inflammation and is thus considered to be a useful inflammatory marker in rats [8,9,[11][12][13][14].…”