The Effects of Interleukin-6 and Cytokine-Induced Neutrophil Chemoattractant-1 on .ALPHA.2-Macroglobulin Production in Rats

Honjo, Toshio; Kuribayashi, Takashi; Seita, Tetsurou; Mokonuma, Yui; Yamaga, Ayaka; Yamazaki, Shunsuke; Yamamoto, Seigo

doi:10.1538/expanim.59.589

Cited by 11 publications

(12 citation statements)

References 24 publications

(17 reference statements)

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“…in the present study, The Cmax was also observed at 48 h after injection of turpentine oil before decreasing slowly in hepatopathic rats. The kinetics of α2M in experimental hepatopathic rats were similar to those in previous reports [8][9][10]13]. This study clarified that serum concentrations of α2M did not increase in hepatopathic rats when compared with nontreated rats.…”

Section: Discussionsupporting

confidence: 89%

“…The Cmax of α2M was observed at 48 h after inflammatory stimulation, e.g., injection of turpentine oil, inoculation with microorganisms or surgical treatment, before decreasing slowly [8][9][10]13]. in the present study, The Cmax was also observed at 48 h after injection of turpentine oil before decreasing slowly in hepatopathic rats.…”

Section: Discussionsupporting

confidence: 62%

“…Turpentine oil is known to induce acute inflammation and has been used to induce acute inflammation in rats and dogs [1,3,6,8,9,12]. in this study, turpentine oil (Wako Pure Chemical industries, Ltd.) was thus used to induce acute inflammation by intramuscular injection at 1.0 ml/kg body weight at 2 days after the end of acetaminophen or CCl 4 administration.…”

Section: Animal Experimentsmentioning

confidence: 99%

“…Introduction α 2 -Macroglobulin (α2M) and α 1 -acid glycoprotein (α 1 -aG) are typical acute-phase proteins in rats [8,9]. Serum concentrations of α2M and α 1 -aG increase by 50-to 290-fold and 34-fold, respectively, compared with pretreatment values in rats during induced acute inflammation [8].…”

mentioning

confidence: 99%

“…Serum concentrations of α2M and α 1 -aG increase by 50-to 290-fold and 34-fold, respectively, compared with pretreatment values in rats during induced acute inflammation [8]. In this way, α2M responds more sensitively than α 1 -AG as a marker of acute inflammation and is thus considered to be a useful inflammatory marker in rats [8,9,[11][12][13][14].…”

mentioning

confidence: 99%

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Impairment of α<sub>2</sub>-Macroglobulin Synthesis in Experimental Hepatopathic Rats Treated with Turpentine Oil

et al. 2012

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Abstract:The aim of this study was to investigate the synthesis of α 2 -macroglobulin (α2M) in hepatopathic rats injected with turpentine oil to induce acute inflammation. Hepatopathy was induced by oral administration of acetaminophen at a dose of 1 g/kg daily for 2 weeks or a 25% solution of carbon tetrachloride (CCl 4 ) at 2 ml/kg body weight three times per week for 7 weeks. Acute inflammation was induced by intramuscular injection of turpentine oil at a dose of 1.0 ml/kg body weight. Serum concentrations of α2M were measured by enzyme-linked immunosorbent assay. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and total protein differed significantly between acetaminophen or CCl 4 -induced hepatopathic rats and acetaminophen control (AA-control) or CCl 4 control (CC-control) rats. Furthermore, pathological examination confirmed hepatopathy in rat livers. Peak serum concentrations and area under the time-concentration curve for α2M showed significant differences between hepatopathic rats and AA-control or CC-control rats. Thus, serum concentrations of α2M did not increase when compared with nontreated rats.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 62%

Section: Animal Experimentsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Impairment of α<sub>2</sub>-Macroglobulin Synthesis in Experimental Hepatopathic Rats Treated with Turpentine Oil

et al. 2012

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Review of immunological plasma markers for longitudinal analysis of inflammation and infection in rat models

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Tucker

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et al. 2022

Journal Orthopaedic Research

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Disease or trauma of orthopedic tissues, including osteomyelitis, osteoporosis, arthritis, and fracture, results in a complex immune response, leading to a change in the concentration and milieu of immunological cells and proteins in the blood. While C-reactive protein levels and white blood cell counts are used to track inflammation and infection clinically, controlled longitudinal studies of disease/injury progression are limited.Thus, the use of clinically-relevant animal models can enable a more in-depth understanding of disease/injury progression and treatment efficacy. Though longitudinal tracking of immunological markers has been performed in rat models of various inflammatory and infectious diseases, currently there is no consensus on which markers are sensitive and reliable for tracking levels of inflammation and/or infection. Here, we discuss the blood markers that are most consistent with other outcome measures of the immune response in the rat, by reviewing their utility for longitudinal tracking of infection and/or inflammation in the following types of models: localized inflammation/arthritis, injury, infection, and injury + infection. While cytokines and acute phase proteins such as haptoglobin, fibrinogen, and α 2 -macroglobulin demonstrate utility for tracking immunological response in many inflammation and infection models, there is likely not a singular superior marker for all rat models. Instead, longitudinal characterization of these models may benefit from evaluation of a collection of cytokines and/or acute phase proteins.Identification of immunological plasma markers indicative of the progression of a pathology will allow for the refinement of animal models for understanding, diagnosing, and treating inflammatory and infectious diseases of orthopedic tissues.

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Comparison of α2-Macroglobulin Synthesis by Juvenile vs. Mature Rats after Identical Inflammatory Stimulation

et al. 2013

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Synthesis of α2-macoglobulin (α2M) by 3-week-old juvenile rats was compared to that of mature 7- and 11-week-old rats. Serum concentrations of α2M, interleukin (IL)-6- and cytokine-induced neutrophil chemoattractant (CINC)-1 were measured by enzyme-linked immunosorbent assay. The area under the concentration vs. time curve (AUC) for α2M was significantly different among the three groups. The synthesis of α2M increased in an age-dependent manner. No significant difference was observed for the AUC of IL-6, but that of CINC-1 in 3-week-old rats was significantly lower than that in 7- or 11-week-old rats. These results suggest that synthesis of α2M was increased in mature compared to juvenile rats, possibly due to differences in liver function. The maximum concentration of CINC-1 in 3-week-old rats was observed 6 h after turpentine oil injection. The serum concentrations of IL-6 and CINC-1 increased more quickly in juvenile rats than in mature rats after inflammatory stimulation.

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The Effects of Interleukin-6 and Cytokine-Induced Neutrophil Chemoattractant-1 on .ALPHA.2-Macroglobulin Production in Rats

Cited by 11 publications

References 24 publications

Impairment of α<sub>2</sub>-Macroglobulin Synthesis in Experimental Hepatopathic Rats Treated with Turpentine Oil

Impairment of α<sub>2</sub>-Macroglobulin Synthesis in Experimental Hepatopathic Rats Treated with Turpentine Oil

Review of immunological plasma markers for longitudinal analysis of inflammation and infection in rat models

Comparison of α2-Macroglobulin Synthesis by Juvenile vs. Mature Rats after Identical Inflammatory Stimulation

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