Kinetics of electron transport chain in isolated mitochondria in response to visible light using resonance Raman spectroscopy

Lalonde, Joshua W.; Pope, Nathaniel J.; Noojin, Gary D.; Yakovlev, Vladislav V.; Denton, Michael L.

doi:10.1117/12.2609712

Cited by 1 publication

(1 citation statement)

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“…8 ROS production in irradiated cells is able to normalize ATP production and regulate proteins involved in cell proliferation and differentiation, in addition to activating several transcription factors. 1,7 Although the effects of light have been investigated from many different types of samples, using diverse light sources, wavelengths, and distinct analysis techniques, 1,2,9,10 there still much to be explored and understood about the effects of photons on mitochondria. With this in mind, this work proposes to study the interaction of photons at different wavelengths in a model of isolated mitochondria.…”

Section: Introductionmentioning

confidence: 99%

Understanding the mechanisms of photobiostimulation on mitochondrial bioenergetics: the challenges of respirometry with real-time irradiation at 635 nm

Mezzacappo¹,

Inada²,

Perez³

et al. 2023

Mechanisms of Photobiomodulation Therapy XVII

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Mitochondria are proposed to be the main target of photobiomodulation (PBM) at the cell level, where photon absorption would lead to therapeutic results. However, there is still plenty to be explored and understood about the effects of photons on mitochondria. This study investigates photon interactions at 635 nm on mitochondrial bioenergetics measuring mitochondrial respiration through high-resolution respirometry (HRR) techniques. Mice (C57BL/6 females) liver mitochondria were isolated and immediately irradiated with a LASER beam emitting at 635 nm while measuring mitochondrial respiration. The selected protocol for respiration analysis allowed the evaluation of oxidative phosphorylation, non-oxidative phosphorylation and the maximal capacity of electron transport chain. The following experimental conditions were applied: temperature (37 and 28 °C), sample concentration (0.5 and 0.25 mg.mL -1 ) and LASER power intensities (200 to 800 mW). At 37 °C and 0.5 mg.mL -1 , the effects were small, but indicated that higher powers might be more promising, and subsequent experiments followed with power intensities ranging from 600 to 800 mW. At 28 °C and 0.25 mg.mL -1 there was a photothermal influence of irradiation, interfering with the oxygen consumption measurements. These results entail the search for a way to correct such effect, aiming to bring the experimental conditions closer to those idealized for this study, that proposes to measure light effects in real time.

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