Kinetics of cytokine secretion by mononuclear cells of the blood from rheumatoid arthritis patients are different from those of healthy controls

Abstract

SUMMARY Mononuclear cells from peripheral blood (PBMC) of rheumatoid arthritis (RA) patients and healthy controls were incubated with α‐CD3. Cytokine secretion from 2h to 72 h of incubation was measured by ELISA, There were no significant differences in secretion of T cell derived IL‐2 and IL‐4 in cultures from RA patients and controls. The macrophage‐derived cytokines, IL‐1β and tumour‐necrosis factor‐α (TNF‐α) were secreted with a steep increase of concentration during the first 16 h of incubation by PBMC from RA patients. PBMC from healthy controls secreted both cytokines at a constantly rising rate with a maximum for TNF‐α at 48 h and for IL‐1β at 72 h. Interferon‐γ (IFN‐γ) is secreted in significantly reduced concentrations by PBMC from untreated RA patients compared with controls. Gold‐salt treatment led to a slightly delayed and enhanced secretion of TNF‐α and IL‐1β, an enhanced secretion of IL‐2 and a restored secretion of IFN‐γ.

Psychosocial adaptation and cellular immunity in breast cancer patients in the weeks after surgery: An exploratory study

“…Kinetic experiments were performed to establish the optimum time point for collection and evaluation of the supernatants. The time points obtained (48 hrs for the cytokines IFN-γ, TNF-α, and IL-12, and 24 hours for IL-2) are in agreement with previous reports for healthy controls assayed under similar conditions (48 – 50). All kits were from Biosource International except for IL-2 (R and D Systems).…”

Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity

“…IFNγ reaches its maximum at 4–5 days, and IL-1β reaches its maximum level at 24 hr, after which a plateau is attained. (Adapted from (Ruschen et al, 1992; van de Veerdonk et al, 2009))…”

Treatment with Tumor Necrosis Factor-α Inhibitors, History of Allergy, and Hypercalcemia Are Risk Factors of Immune Reconstitution Inflammatory Syndrome in HIV-Negative Pulmonary Tuberculosis Patients

“…In this study, TNFIs were associated with IRIS development, except the immunosuppressive drugs without TNFIs. Interestingly, RA and Crohn's disease are Th1 cell-dominant diseases [20][21][22][23], and TNFI promotes the Th2 cytokine-dominant balance via inhibition of TNF-α. The Th2 cytokine-dominant balance caused by TNFI at the initial treatment of TB may be partly associated with IRIS development.…”