“…Recently, data‐independent acquisition mass spectrometry has been applied for HLA‐I peptide discovery,41, 42 which, however, needs compatible instrumentation. Another possibility that was recently utilized for sensitive detection as well as quantification of HLA‐I peptides is targeted data acquisition 38, 43, 44, 45, 46, 47. Also in the present study, we used a targeted LC‐MS 3 approach, which is more sensitive for detection of HLA‐presented peptides than untargeted methods due to pre‐defined and pre‐programed analytes, resulting in longer acquisition times per analyte for increased sensitivity.…”