Kinetics and type of immune response following intranasal and subcutaneous immunisation of calves

Rebelatto, M. C.; Siger, L.; HogenEsch, Harm

doi:10.1053/rvsc.2001.0477

Cited by 13 publications

(5 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, alternative mucosal routes of IsdA-CTA 2 /B delivery will be explored for the development of a potential human vaccine. In addition, S. aureus is the major cause of bovine mastitis in dairy cattle, and significant doses of CTB have been intranasally administered to cows without signs of neuronal toxicity (3,45). The potential of the IsdA-CTA 2 /B chimera for use as an intranasal veterinary vaccine to prevent bovine mastitis is also under investigation.…”

Section: Discussionmentioning

confidence: 99%

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A₂/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Arlian

Tinker

2011

Clin. Vaccine Immunol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A₂/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Arlian

Tinker

2011

Clin. Vaccine Immunol.

View full text Add to dashboard Cite

“…The plates were subsequently incubated for 90 h at 37°C in a 5% CO 2 humidified environment, and then pulsed with 0.5 Ci 3 H-thymidine in 50 L of media for the final 18 h of culture. Stimulation indices (Rebelatto et al, 2001) were calculated by dividing the mean counts per minute (cpm) of the triplicate wells containing ConA or Limulus hemocyanin by the mean cpm of the wells containing complete medium only.…”

Section: Whole Blood Lymphocyte Proliferation Assay For the In Vivo Lmentioning

confidence: 99%

“…Serum samples were analyzed for Limulus hemocyanin-specific IgG1 and IgG2 according to the method of Rebelatto et al (2001) with some modifications. Individual wells of 96-well plates were coated with 100 L/ well of Limulus hemocyanin (10 g/mL) suspended in carbonate buffer (pH 9.6) for 24 h at 4°C.…”

Section: Enzyme-linked Immunosorbent Assay For Antigen-specific Antibmentioning

confidence: 99%

Leptin alters antibody isotype in the pig in vivo, but does not regulate cytokine expression or stimulate STAT3 signaling in peripheral blood monocytes in vitro1

Weber

Spurlock

2004

Journal of Animal Science

View full text Add to dashboard Cite

Although leptin modulates immunological pathways in some species, the role of leptin as a regulator of immunocyte function in the pig has not been studied. Therefore, the primary objective of this study was to determine whether leptin influences specific immunocyte response variables in the pig in vivo or in vitro. Fifteen pigs (five pigs per treatment) were 1) injected with recombinant human leptin and allowed to consume feed ad libitum, 2) injected with vehicle and allowed to consume feed ad libitum, or 3) injected with vehicle and limit-fed to the intake of the leptin-injected group. All the pigs also were injected with the antigen, Limulus hemocyanin, on d 0 and 15 of the experiment. Exogenous leptin decreased (P < 0.05) daily feed intake and antigen-specific immunoglobulin (Ig) G1, but had no effect on lymphocyte proliferation or antigen-specific IgG2. In a second series of experiments, peripheral blood mononuclear cells (PBMC) were isolated from venous blood to determine the effect of stimulation with the polyclonal mitogen, concanavalin A (ConA), on the long form of the leptin receptor (Ob-Rl) mRNA abundance, and to determine whether leptin altered mitogen-induced proliferation, cytokine production, or signal transducer and activator of transcription 3 (STAT3) activation. Leptin had no effect on the proliferation of PBMC or on cytokine mRNA abundance or secretion. The abundance of Ob-Rl mRNA was decreased (P < 0.05) in response to stimulation with ConA. Constitutive STAT3 DNA binding was evident in mobility shift assays, but was not altered by either leptin or serum deprivation. These data indicate that leptin modifies antibody isotypes in the pig, and that Ob-Rl expression is downregulated in response to polyclonal mitogens in porcine PBMC. The constitutive activation of STAT3, coupled with the absence of leptin-inducible binding, indicates an alternative signaling pathway for leptin in pig PBMC.

show abstract

“…In a similar study in cattle, antigen‐specific mucosal immune induction was reported following i.n. immunization with CT as the vaccine adjuvant, although in this study vaccine formulations were delivered to the nasal mucosa by aerosol spray 43 . Based on the findings from these immunization studies in ruminants, it appears that immunization at the nasal and rectal mucosa may be suitable alternatives to traditional oral immunization for the induction of specific mucosal immune responses.…”

Section: Induction Of Specific Immune Responses Following Intrarectalmentioning

confidence: 99%

Alternative routes of mucosal immunization in large animals

2004

View full text Add to dashboard Cite

Summary Mucosal immunization regimes that employ the oral route of delivery are often compromised by antigen degradation in the stomach. Moreover, tolerance or immunological unresponsiveness to orally delivered vaccine antigens is also a major problem associated with this route of immunization. Immunization by alternative routes including intrarectal (i.r.) and intranasal (i.n.) is becoming increasingly recognized in large animals for generating protective antibody responses at mucosal surfaces. These approaches are particularly useful in ruminant species which have four stomachs that can potentially interfere with antigen presentation to mucosal inductive sites of the gut. Modifications to enhance existing mucosal immunization regimes have also been explored through the use of alternative antigen delivery systems and mucosal adjuvants. The combination of alternative immunization routes and the use of appropriate antigen delivery systems appear to be a rational approach for providing protective immunity at mucosal surfaces. There has been a considerable amount of research conducted on evaluating the efficacy of emerging antigen delivery systems and novel adjuvants for improved immunity to mucosal immunization but very little of this work has been specific to the mucosal compartment of large animals. The aim of this review is therefore to assess the feasibility and practicality of using large animals (particularly sheep, cattle and pigs) for inducing and detecting specific immune responses to alternative mucosal routes of immunization.

show abstract

Kinetics and type of immune response following intranasal and subcutaneous immunisation of calves

Cited by 13 publications

References 49 publications

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A₂/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A₂/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Leptin alters antibody isotype in the pig in vivo, but does not regulate cytokine expression or stimulate STAT3 signaling in peripheral blood monocytes in vitro1

Alternative routes of mucosal immunization in large animals

Contact Info

Product

Resources

About

Kinetics and type of immune response following intranasal and subcutaneous immunisation of calves

Cited by 13 publications

References 49 publications

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A2/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A2/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Leptin alters antibody isotype in the pig in vivo, but does not regulate cytokine expression or stimulate STAT3 signaling in peripheral blood monocytes in vitro1

Alternative routes of mucosal immunization in large animals

Contact Info

Product

Resources

About

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A₂/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice

Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A₂/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice