Kinetic study of glomerular cells of diabetic rats in relation to the development of irreversible glomerular sclerosis

Osawa, Gengo; Sasaki, Tatsuya; Sato, Tetsuya; Yamada, Masahiko; Kitano, Yuichi

doi:10.1016/0891-6632(91)90038-q

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…Therefore they are not exact replicas of human diabetes, so useful spontaneously diabetic strains must be developed. This spontaneously diabetic WBN/Kob rat is useful in the study of chronic complications of diabetes mellitus, and some studies on the WBN/Kob rat as a diabetic model animal have been reported [21][22][23][24]. In this study, we demonstrated two types of changes in the eyes of WBN/Kob rats: cataract caused by diabetes mellitus and retinal degeneration which may be due to hereditary factors.…”

Section: Discussionmentioning

confidence: 70%

Lens and Retinal Changes in the WBN/Kob Rat (Spontaneously Diabetic Strain)

Miyamura

Amemiya²

1998

Ophthalmic Res

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Male WBN/Kob rats represent a spontaneously diabetic strain with hyperglycemia, cataracts, nephropathy, neurophathy, pancreatic fibrosis and hyperlipemia. Cataracts and retinal changes in WBN/Kob rats were examined by light and electron microscopy to evaluate the ocular complications. Lens opacity was present in the posterior subcapsular and center of the anterior cortex of male 14-month-old WBN/Kob rats. Light and transmission electron microscopy showed swelling and irregularity of lens fibers. Scanning electron microscopy revealed that lens fibers were irregular and had many granules and bulging processes of various sizes on the cortical side of the opacified region. The nuclear side of the opacified region showed spongy changes and complete absence of lens fibers. Electron microscopy showed retinal degeneration in the photoreceptor outer segments of 1-month-old male WBN/Kob rats. Light microscopy showed thin outer segments and outer nuclear layers in 5-month-old rats, and electron microscopy revealed severe degeneration in the outer segments. The retinas of 11-month-old rats were thinner; the outer plexiform layer was very thin; the photoreceptor cell nuclei in the outer nuclear layer had decreased to one layer and were almost in contact with the inner nuclear layer nuclei, while the visual cells had disappeared. Retinal degeneration had progressed even further in 14-month-old rats, and very few photoreceptor cell nuclei remained. The retinal capillary lumens were small, and their pericytes had thickened basement membranes. The basement membranes of retinal capillaries from WBN/Kob rats were significantly thicker than those from control Wistar rats (p < 0.0001). Although this rat has spontaneous diabetic features, such as cataracts, its retinal changes look more degenerative.

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Section: Discussionmentioning

confidence: 70%

Lens and Retinal Changes in the WBN/Kob Rat (Spontaneously Diabetic Strain)

Miyamura

Amemiya²

1998

Ophthalmic Res

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“…The glomerulus contains three cell populations. The mesangial (MES) and the endothelial cells (END) can both proliferate (22), whereas there is strong evidence that the podocyte or epithelial cell (EP) is incapable of regenerative replication postnatally (23). If this is the case, the present study shows that the podocyte must enlarge or spread in order to cover the increasing surface area (68%) caused by diabetes.…”

Section: Discussionmentioning

confidence: 99%

Glomerular structural changes in pregnant, diabetic, and pregnant‐diabetic rats

Rasch¹,

Lauszus

Thomsen³

et al. 2005

APMIS

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Kidneys enlarge both during pregnancy and in diabetes. The enlargement and morphology of glomeruli was studied during pregnancy and in diabetes in order to examine possible similarities, differences, and interactions in the growth in these conditions. Morphometric investigations were performed on glomeruli in pregnant rats, in rats with 2 weeks' diabetes, and in pregnant-diabetic rats. Kidneys were enlarged 22% in the midterm pregnant rats compared with controls, 74% in diabetic rats, and a further 21% in pregnant-diabetic rats. Glomerular volume was enlarged by 26% during midterm pregnancy in normal animals. Diabetes induced an enlargement in glomerular volume of 58% and a further 18% in midterm diabetic animals due to pregnancy. Within the glomerulus, pregnancy in normal animals induced minor non-significant changes. Diabetes induced significant increase in several parameters: mesangial volume and cell volume, capillary and glomerular basement membrane volume, capillary wall surface area, foot process width, filtration slit length, and nuclear number. Pregnancy in diabetic animals induced no significant additional changes. In conclusion, kidney enlargement in pregnancy shows very few glomerular changes in either normal or diabetic animals. Enlargement of glomeruli in diabetes involves hypertrophy and hyperplasia concurrent with several morphological changes within the glomerulus.

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Kinetic study of glomerular cells of diabetic rats in relation to the development of irreversible glomerular sclerosis

Cited by 2 publications

References 2 publications

Lens and Retinal Changes in the WBN/Kob Rat (Spontaneously Diabetic Strain)

Lens and Retinal Changes in the WBN/Kob Rat (Spontaneously Diabetic Strain)

Glomerular structural changes in pregnant, diabetic, and pregnant‐diabetic rats

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