Kinetic Studies of Ammonia Monooxygenase Inhibition in
            <i>Nitrosomonas europaea</i>
            by Hydrocarbons and Halogenated Hydrocarbons in an Optimized Whole-Cell Assay

Keener, William K.; Arp, Daniel J.

doi:10.1128/aem.59.8.2501-2510.1993

Cited by 147 publications

(75 citation statements)

References 25 publications

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“…The increased sensitivity of potential nitrification activity may be explained by the inhibitory effect of aliphatic compounds on the ammonia monooxygenase (AMO) enzyme. Keener and Arp [7] suggest that hydrocarbons cause inhibition of AMO through competitive or noncompetitive interactions with the enzyme. The competitive nature of the inhibitor generally decreases as molecular size increases (i.e., small hydrocarbon molecules are capable of competing with ammonia for the active binding site on the AMO enzyme).…”

Section: Discussionmentioning

confidence: 99%

“…The competitive nature of the inhibitor generally decreases as molecular size increases (i.e., small hydrocarbon molecules are capable of competing with ammonia for the active binding site on the AMO enzyme). Keener and Arp suggest that AMO inhibition by hydrocarbon could occur either by directly competing with ammonia at the AMO binding site for reducing equivalents or by binding to a hydrophobic region of the AMO enzyme and influencing enzyme turnover [7]. Polyaromatic hydrocarbons, such as naphthalene, also can irreversibly inhibit AMO [37].…”

Section: Discussionmentioning

confidence: 99%

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Soil biogeochemical toxicity end points for sub‐Antarctic islands contaminated with petroleum hydrocarbons

Schafer

Snape

Siciliano

2007

Enviro Toxic and Chemistry

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Sub-Antarctic islands have been subjected to petroleum hydrocarbon spills, yet no information is available regarding the toxicity of petroleum hydrocarbons to these subpolar soils. The purpose of the present study was to identify soil biogeochemical toxicity end points for petroleum hydrocarbon contamination in sub-Antarctic soil. Soil from Macquarie Island, a sub-Antarctic island south of Australia, was collected and exposed to 10 concentrations of Special Antarctic Blend (SAB) diesel fuel, ranging from 0 to 50,000 mg fuel/kg soil, for a 21-d period. The sensitivity of nitrification, denitrification, carbohydrate utilization, and total soil respiration to SAB fuel was assessed. Potential nitrification activity was the most sensitive indicator of SAB contamination assessed for nitrogen cycling, with an IC20 (concentration that results in a 20% change from the control response) of 190 mg fuel/ kg soil. Potential denitrification activity was not as sensitive to SAB contamination, with an IC20 of 950 mg fuel/kg soil for nitrous oxide production. Nitrous oxide consumption was unaffected by SAB contamination. Carbohydrate utilization (respiration caused by sucrose) was a more sensitive indicator (IC20, 16 mg fuel/kg soil) of SAB contamination than total respiration (IC20, 220 mg fuel/kg soil). However, total soil respiration was a more responsive measurement end point, increasing soil respiration over a 72-h period by 17 mg of CO2, compared to a change of only 2.1 mg of CO2 for carbohydrate utilization. Our results indicate that IC20s varied between 16 to 950 mg fuel/kg soil for Macquarie Island soil spiked with SAB diesel fuel. These results indicate that current cleanup levels derived from temperate zones may be too liberal for soil contamination in sub-Antarctic islands.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Soil biogeochemical toxicity end points for sub‐Antarctic islands contaminated with petroleum hydrocarbons

Schafer

Snape

Siciliano

2007

Enviro Toxic and Chemistry

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“…Chem. 16,1997 J.L. Miller et al King et al [10] have also shown no effect of a chlorinated solvent on degradation of an organic substrate.…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, the detection of both nitrite and nitrate in samples after 2 months of additional incubation and the growth of nitrifying bacteria in liquid culture medium indicate that inhibition of nitrification may similarly be due to this interaction with a key enzyme. Keener and Arp [16] have shown that the halogenated alkanes are noncompetitive substrates for AMO and have proposed a model of AMO that contains two substrate binding sites. In this model, one site will accommodate C 1 and C 2 hydrocarbons as well as NH 3 , while the other, more hydrophobic site has a wider substrate range that Effect of application solvents on nitrification Environ.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Application Solvents on Heterotrophic and Nitrifying Populations in Soil Microcosms

Miller

Sardo

Thompson

et al. 1997

Environ Toxicol Chem

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Agricultural practices may cause contamination of soil and ground water with a combination of organic compounds (pesticides and fuel) and nitrogen fertilizers. In coupled microcosm studies that monitored the mineralization of naphthalene and the nitrification of ammonia, it was observed that the solvent (dichloromethane) used to apply naphthalene to the soil inhibited nitrification, although there was no effect on naphthalene mineralization. Further studies were performed with a series of application solvents: methanol, acetonitrile, trichloromethane, and dichloromethane. Soil and solvent were allowed to equilibrate with ambient air for various times before capping and incubation of microcosms. Results indicated that dichloromethane equilibrated for 5 mins inhibited nitrification for at least 3 weeks relative to the control (water). Acetonitrile and trichloromethane similarly inhibited nitrification. Methanol and dichloromethane equilibrated for 60 mins also significantly delayed nitrification, although to a lesser extent. Inhibition of nitrification was not permanent, and nitrification activity was eventually restored in all systems tested. None of the solvents inhibited mineralization of the added carbon source. These results indicate that special care must be taken to ensure that applications solvents do not affect the activity of sensitive microbial populations, such as the nitrifiers, that may be part of a study.

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Nitrification and Denitrification‐Microbial Fundamentals and Consequences for Application

Zart¹,

Stüven²,

Bock³

1999

Biotechnology

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Kinetic Studies of Ammonia Monooxygenase Inhibition in Nitrosomonas europaea by Hydrocarbons and Halogenated Hydrocarbons in an Optimized Whole-Cell Assay

Cited by 147 publications

References 25 publications

Soil biogeochemical toxicity end points for sub‐Antarctic islands contaminated with petroleum hydrocarbons

Soil biogeochemical toxicity end points for sub‐Antarctic islands contaminated with petroleum hydrocarbons

Effect of Application Solvents on Heterotrophic and Nitrifying Populations in Soil Microcosms

Nitrification and Denitrification‐Microbial Fundamentals and Consequences for Application

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