Kinetic Spectrophotometric Methods for the Determination of Chloramphenicol in Pharmaceutical Preparations

Al-Ward, Hind S.

doi:10.22401/jnus.15.4.03

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…The results of the proposed method for three type of samples in this study were compared statistically with reported method that used metacresol purple (MCP) as a chromogenic reagent and spectrophotometric determination of SC (32) . The results are summarized from Table 11.…”

Section: * Average Of Five Determinationsmentioning

confidence: 99%

Extraction and Determination of Sildenafil Citrate by Spectrophotometry: Classical Versus Factorial Design Optimization

A-A Khammas

2023

Tikrit J. Pharm. Sci.

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In this work, the complexation and extraction processes of sildenafil citrate (SC) were evaluated by using classical optimization versus factorial design methodology. The selected factors were based on the reaction of sildenafil with methyl orange (MO) as a chromogenic reagent via the formation of ion-association complex in acidic buffer media, followed by extraction with chloroform and quantitatively measured by visible spectrophotometry at λmax of 427 nm. The optimization step was first carried out by classical one-factor-at-a-time (OFAT) from which the extracted results were exploited in the experimental design optimization by using 24-1 fractional factorial and 32 full factorial designs for the chosen variables such as pH, concentration of reagent, reaction time, extraction time and temperature. Results obtained from fractional factorial design 24-1 showed that only the variables pH, MO concentration and their interaction based on analysis of variance (ANOVA) in term of Pareto chart, were statistically significant at 95% confidence level. Under the optimized conditions, the assay method of SC was validated in term of the analytical figures of merit. SC can be determined in concentration range of 0.5-40 µg mL-1 with correlation coefficient of 0.9991, detection limit (S/N) of 0.15 µg mL-1, RSD (n=10) of 1.43%, accuracy as %Erel of -2.23% and mean percent recovery of 97.77±0.87. The proposed method was applied for the determination of SC in commercial medicaments without fear from excipients interferences with the assay procedure.

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Section: * Average Of Five Determinationsmentioning

confidence: 99%

Extraction and Determination of Sildenafil Citrate by Spectrophotometry: Classical Versus Factorial Design Optimization

A-A Khammas

2023

Tikrit J. Pharm. Sci.

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“…For the determination of studied drug, various methods have been reported in literature; these methods included: High performance liquid chromatography (HPLC) which is one of the most powerful and versatile tool for the quantitative determination of CAP (Hoang et al, 2015;Suguna et al, 2014;Mallu et al, 2011), also LC-MS (Bjorn, 2013), LC-MS-MS (Rocha et al, 2015). Other analytical methods have been reviewed in literature these methods included: voltammetry (Yafeng et al, 2014), Polarography (Suliman and Razzak, 2000), so that various spectrophotometric methods have been used (Alshirifi and Alhameedi, 2016;Suguna et al, 2016;Wafi et al, 2015;Al-Abachi et al, 2014;Al-Sabha and Al-Hammoshi, 2013;Sinan and Al-Abachi, 2010;Sayhood et al, 2013;Al-Sabha and Rasheed, 2010;Al-Ward, 2012).…”

Section: Cap Structure Mwt = 3231322 G / Molmentioning

confidence: 99%

Using of 2,7-Dihydroxynaphthalene as a Novel Reagent in Spectrophotometric Assay of Chloramphenicol

Othman¹,

Jamal²

2019

RJS

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An indirect spectrophotometric method for the determination of chloramphenicol (CAP) has been suggested. The method is based on the oxidative coupling reaction of reduced chloramphenicol (R-CAP) with 2,7-dihydroxynaphthalene (2,7-DHN) reagent in the presence of potassium dichromate as oxidizing agent to produce brown colored, stable and soluble product. This product showed maximum absorption at 544 nm. Beer's law is obeyed over the range 0.5-7.5.µg.ml-1 of R-CAP and molar absorptivity of 1.069×10 5 l.mol-1 .cm-1 and Sandell's sensitivity index of 0.0030 µg.cm-2 , limit of detection (LOD), limit of quantitation (LOQ), relative error (RE%) and relative standard deviation (RSD%) have been estimated. The method has been successfully applied to the determination of CAP in drug formulations.

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“…Furthermore, BH-HCl is one of the mucous-modifying drugs that aid expectoration by improving the flow characteristics of bronchial mucous [3,4]. A literature review indicated the determination of bromhexine hydrochloride in dosage form, either in combined form or alone, by spectrophotometric [5][6][7][8][9][10][11], potentiometric [12], voltammetric [13], colorimetric [14], chemiluminescence [15,16], TLC [17], HPLC [18][19][20][21][22][23][24][25], RRLC-MS/MS [26], and HPTLC-HPLC [27]. This study presents a new method to determine BH-HCl in pharmaceutical formulations using tetraphenylborate as a precipitating reagent to form a yellowish-white precipitate with the use of a homemade instrument.…”

Section: Introductionmentioning

confidence: 99%

CFIA-ISNAG fluorimeter for the Determination of Bromhexine-HCl in Drugs via the Measurement Scattered Light at ± 90˚

Al-Saadi,

Al-Awadie

2023

Iraqi Journal of Science

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A precise, simple, and accurate continuous flow injection technique was used for the instantaneous estimation of bromhexine hydrochloride (BH-HCl) in tablet dosage form. The chemical and physical parameters of the reaction of BH-HCl with tetraphenylborate to produce a yellowish-white precipitate were determined using an ISNAG fluorimeter analyzer and diverging light at 90°. The calibration curve for BH-HCl was linear with correlation coefficients of 0.9994 and linearity percentage r2% = 99.87 over a concentration range of 0.01-20 mmol/L, L.O.D = 0.3610 µg/125µL (0.007 mmol/L), and RSD% less than 0.3% for 3 and 13 mmol/L (five replicates). This approach was efficiently used to estimate the levels of BH-HCl in two distinct pharmaceutical companies. When the newly developed method was compared with the turbidimetric method using a t-test and standard addition, it was discovered that there had not been any significant difference between the two approaches at the 95% level of confidence.

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Kinetic Spectrophotometric Methods for the Determination of Chloramphenicol in Pharmaceutical Preparations

Cited by 4 publications

References 16 publications

Extraction and Determination of Sildenafil Citrate by Spectrophotometry: Classical Versus Factorial Design Optimization

Extraction and Determination of Sildenafil Citrate by Spectrophotometry: Classical Versus Factorial Design Optimization

Using of 2,7-Dihydroxynaphthalene as a Novel Reagent in Spectrophotometric Assay of Chloramphenicol

CFIA-ISNAG fluorimeter for the Determination of Bromhexine-HCl in Drugs via the Measurement Scattered Light at ± 90˚

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