Kinetic Spectrophotometric Determination of Ranitidine

Abstract

Two spectrophotometric methods were developed for the determination of ranitidine. The first method was a kinetic spectrophotometric method based on the catalytic effect of ranitidine on the reaction between sodium azide and iodine in an aqueous solution. The calibration graph was linear from 4-24 µg/mL. The drug was determined by measuring the decrease in the absorbance of iodine at 348 nm using a fixed time method. The decrease in the absorbance after 1 minute from the initiation of the reaction was related to the concentration of drug. The detection limit of the procedure was 0.76 mg/mL. The proposed procedure was successfully utilized in the determination of the drug in pharmaceutical preparations with mean recovery in the range of 99.83 -101.16% .The second method is a colorimetric method, which depends on the measurement of absorbances of tris (o-phenanthroline) iron(II) [method 2A] and tris (bipyridyl) iron(II) [method 2B] complexes at 512 nm. The complexes obeyed Beer's law over the concentration range of 2-16 mg/mL and 4-40 mg/mL for methods 2A and 2B, respectively. The developed method has been successfully applied for the determination of ranitidine in bulk drugs and pharmaceutical formulations. The common excipients and additives did not interfere in its determination.

Spectrophotometric method for determination of ranitidine hydrochloride in bulk and in pharmaceutical preparation using ninhydrin

“…By making comparison between our proposed method and with earlier methods [1,3,19]. We found that our method is suitable with high sensitivity for determination of RNH.…”

“…It is a H2receptor antagonist and is widely used for the short-term treatment of duodenal ulcers and for the management of hypersecretory conditions [1]. Ranitidine hydrochloride has three different substitution amine group, nitro group, furan ring, and sulfur group with hydrochloride [2,3].…”

Instrumental analysis of some anti-ulcer drugs in different matrices

“…In the second method; complexes were formed between (o-phenanthroline) iron (II) and tris (bipyridyl) iron (II) measuring the absorbances at λ max 512 nm. [10] Potassium dichromate was used as oxidimetric reagent in spectrophotometric determination of ranitidine in pharmaceuticals. [11] N-bromosuccinimide (NBS) was used as an analytical reagent for the determination of ranitidine HCl.…”

Amperometric Detection of Ranitidine Using Glassy Carbon Modified with Ruthenium Oxide Hexacyanoferrate Adapted in a Flow Injection System

“…In the literature, there are different methods for quantification of ranitidine using the following techniques: spectrophotometry [3][4][5][6][7][8][9][10][11], fluorimetry [7,12,13], chemiluminescence [14,15], spectrometry [16], electrochemistry [11,[17][18][19][20][21][22][23][24][25] and chromatography [26][27][28]. In most cases, using the methods of spectrophotometry, spectrometry, fluorimetry and chemiluminescence a preliminary step of derivatization is necessary, which will result in longer analysis times, an example of which is the precipitation of ranitidine with Co and Cd complex prior to analysis by spectrometry [16].…”