Kinetic resolution of a drug precursor by Burkholderia cepacia lipase immobilized by different methodologies on superparamagnetic nanoparticles

Production of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Tris(hydroxymethyl)phosphine as an Optional Coupling Agent

“…The use of magnetic nanoparticles as a support for enzyme immobilization has several advantages: (1) a higher specific surface area permitting the binding of a larger amount of enzymes; (2) the mass transfer resistance being relatively low; and (3) easy and selective separation of the immobilized enzyme from a reaction mixture by the application of a magnetic field [ 20 , 21 ]. Magnetic nanoparticles have been used for the immobilization of many enzymes, such as lipase [ 22 ], chitosanase [ 23 ], d -amino acid oxidase [ 24 ], β-galactosidase [ 25 ], and β-fructofuranosidase [ 26 ].…”

Synthesis of polyethyleneimine (PEI) and β‐cyclodextrin grafted PEI nanocomposites with magnetic cores for lipase immobilization and esterification

“…Covalent attachment and physical adsorption are the two main routes applied for the immobilization of enzymes on different supports . Despite its rather simple nature, the direct adsorption of lypolytic enzymes such as TLL onto the functionalized surface of the nanoparticles is a much more appropriate method of immobilization than covalent attachment, which can inactivate the desired enzyme . On the other hand, TLL has only seven lysine residues, and the application of covalent attachment via the alteration of the amino group using epoxy, glyoxyl, and glutaraldehyde may be difficult to achieve .…”

Reuse of Candida Antartica Lipase B Immobilized Onto Iron Magnetic Nanoparticles for Synthesis of Flavor Esters

“…Aiming to immobilize CALB onto iron magnetic nanoparticles, the support was initially treated with γ-aminopropyltriethoxysilane solution, according to the methodology described by Netto et al (2009). After the treatment was finished, the cross-linking of the support was done with glutaraldehyde solution, as described by Andrade et al (2010). Once this was done, immobilization of the enzyme on the support was started by the contact of 0.01 g of magnetic nanoparticles with 0.5 mL of sodium phosphate buffer (100 mM and pH 7.0), the system was maintained under controlled stirring at 45 rpm.…”