Reuse of Candida Antartica Lipase B Immobilized Onto Iron Magnetic Nanoparticles for Synthesis of Flavor Esters

Abstract

Enzymes are biocatalysts that allow natural catalysis under mild reaction conditions and are highly selective. In order to increase enzyme stability and facilitate its recovery and reuse, enzymes are immobilized onto magnetic nanoparticles. In this context, this study aims to analyze the maintenance of the catalytic activity of Candida antartica Lipase B immobilized onto magnetic nanoparticles applied to the production of methyl butyrate and ethyl butyrate through esterification. Analyzing the effects of temperature, the best conversion rate was 78.48% for ethyl butyrate at 35 °C, while the conversion rate for methyl butyrate was 80.67% at a substrate concentration of 0.5 mol/L. Analyzing the molar ratio (acid: alcohol), the best conversion rate (44.71%) was obtained for ethyl butyrate using a molar ratio of 1:1.