Kinetic properties of phosphofructokinase of Neurospora crassa

Tsao, Makepeace U.; Madley, T. I.

doi:10.1016/0005-2744(72)90969-2

Cited by 15 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The properties of PFK have been reported for sheep brain, liver fluke, guinea pig heart, turtle heart, skeletal muscle, Escticrichin coli, rat and rabbit RBC's, rat liver and Neurospora cvassa (1,4,(14)(15)(16)(17)(18)(19)26). It is generally accepted that this en-7yme is the major regulator of glycolysis and is responsible for the Pastrur rffect.…”

Section: Discussionmentioning

confidence: 99%

Phosphofructokinase of Leishmania donovani and Leishmania braziliensis and its Role in Glycolysis*

Berens

Marr

1977

The Journal of Protozoology

View full text Add to dashboard Cite

It was shown in an investigation of the phosphofructokinases of Leishmania donovani and Leishmania braziliensis that both enzymes are similar to that of Crithidia fasciculata. Although the enzymes are allosteric with respect to their substrates and require AMP for activation, there is no influence by other heterotropic modifiers. The Mg2+-ATP chelate activates these enzymes in a first order process and they can be inhibited by free ATP. The inhibition is reversed by the activator, AMP, in a competitive manner. The requirement for the nucleotide in L. donovani can be eliminated by decreasing the pH. The data indicate that phosphofructokinase, a pivotal enzyme in glycolysis for most organisms, probably does not play an important role in glycolysis in Leishmania.

show abstract

Section: Discussionmentioning

confidence: 99%

Phosphofructokinase of Leishmania donovani and Leishmania braziliensis and its Role in Glycolysis*

Berens

Marr

1977

The Journal of Protozoology

View full text Add to dashboard Cite

show abstract

“…Unlike the majority of phosphofructokinases, purine nucleotides are better substrates than pyrimidine nucleotides for the phosphofructokinases from Brussels sprouts (27) and Neurosporu crmsu (28).…”

Section: Substrate! Specificitymentioning

confidence: 99%

Phosphofructokinase

Uyeda¹

1979

Advances in Enzymology - And Related Areas of Molecular Biology

239

View full text Add to dashboard Cite

“…The loss function does not help to identify how these reactions/enzymes might be regulated. In experimental studies of Neurospora crassa , PFK in Neurospora crassa is inhibited by high concentrations of ATP [ 36 ]. Early studies on Neurospora PDHm indicated that PDHm is regulated by acetyl-CoA and covalently by phosphorylation [ 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

Prediction of Metabolite Concentrations, Rate Constants and Post-Translational Regulation Using Maximum Entropy-Based Simulations with Application to Central Metabolism of Neurospora crassa

et al. 2018

View full text Add to dashboard Cite

We report the application of a recently proposed approach for modeling biological systems using a maximum entropy production rate principle in lieu of having in vivo rate constants. The method is applied in four steps: (1) a new ordinary differential equation (ODE) based optimization approach based on Marcelin's 1910 mass action equation is used to obtain the maximum entropy distribution; (2) the predicted metabolite concentrations are compared to those generally expected from experiments using a loss function from which post-translational regulation of enzymes is inferred; (3) the system is re-optimized with the inferred regulation from which rate constants are determined from the metabolite concentrations and reaction fluxes; and finally (4) a full ODE-based, mass action simulation with rate parameters and allosteric regulation is obtained. From the last step, the power characteristics and resistance of each reaction can be determined. The method is applied to the central metabolism of Neurospora crassa and the flow of material through the three competing pathways of upper glycolysis, the non-oxidative pentose phosphate pathway, and the oxidative pentose phosphate pathway are evaluated as a function of the NADP/NADPH ratio. It is predicted that regulation of phosphofructokinase (PFK) and flow through the pentose phosphate pathway are essential for preventing an extreme level of fructose 1,6-bisphophate accumulation. Such an extreme level of fructose 1,6-bisphophate would otherwise result in a glassy cytoplasm with limited diffusion, dramatically decreasing the entropy and energy production rate and, consequently, biological competitiveness.

show abstract

Kinetic properties of phosphofructokinase of Neurospora crassa

Cited by 15 publications

References 36 publications

Phosphofructokinase of Leishmania donovani and Leishmania braziliensis and its Role in Glycolysis*

Phosphofructokinase of Leishmania donovani and Leishmania braziliensis and its Role in Glycolysis*

Phosphofructokinase

Prediction of Metabolite Concentrations, Rate Constants and Post-Translational Regulation Using Maximum Entropy-Based Simulations with Application to Central Metabolism of Neurospora crassa

Contact Info

Product

Resources

About