Kinetic of the production of polygalacturonase and pectin lyase by two closely relatedFormae speciales ofFusarium oxysporum

Blais, Patrice; Rogers, Peter A. W.; Charest, Pascale G.

doi:10.1016/0147-5975(92)90036-q

Cited by 14 publications

(5 citation statements)

References 15 publications

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“…The basis of the method is that pectin lyase activity is higher at high pH and is stimulated in the presence of calcium ions. These factors lower the activity of polygalacturonase, which is inhibited at higher calcium concentrations (Blais et al, 1992). Hence, the use of these parameters allows the suppression of PG activity and an increase in PL activity.…”

Section: Discussionmentioning

confidence: 99%

**Morphological and biochemical characterization of Armillaria isolates from Zimbabwe**

Mwenje

Ride

1996

Plant Pathology

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Variations in cultural and biochemical characteristics have been used to group 20 Armillaria isolates collected from different hosts and regions in Zimbabwe. Morphological features, including colony type and rhizomorph characteristics in vitro, rhizomorph characteristics from inoculum buried in soil, and features of basidiocarps produced in vitro on wood, millet and oranges, divided the Zimbabwean isolates into three morphologically distinct groups, designated I, II and III. Biochemical attributes involving isozyme and protein patterns studied by electrophoresis and isoelectric focusing also indicated three groups which corresponded precisely with the three groups observed for morphological studies. Isozyme analyses of pectin lyase (PL), pectin methylesterase (PME), beta‐1,3‐glucanase, nonspecific esterases and putative suberinases were important in grouping the isolates. Pectin lyase and pectin methylesterase patterns gave the clearest and mostly easily interpreted results. Total protein patterns by SDS–polyacrylamide gel electrophoresis also showed some group‐specific bands. Cluster and principal component analyses of the data confirmed the separation of the Zimbabwean Armillaria isolates into three distinct groups.

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Section: Discussionmentioning

confidence: 99%

**Morphological and biochemical characterization of Armillaria isolates from Zimbabwe**

Mwenje

Ride

1996

Plant Pathology

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“…Many fungi are known to secrete multiple forms of pectinases, the advantages of which being the ability to confer flexibility and a greater adaptive capacity [6,39, 401. Pectin lyase is usually induced at pH 6.5 to 8 in other fungi [22,24,[41][42][43], but production of pectin lyase by T. trogii was obtained when the initial pH of the medium was 3.3. Decomposition of plant cell walls during tissue invasion and pathogenesis is a characteristic feature of numerous plant diseases caused by phytopathogenic fungi.…”

Section: Culture Age [Days]mentioning

confidence: 99%

Culture conditions for the production of pectinolytic enzymes by the white‐rot fungus Trametes trogii on a laboratory scale

Levin

Forchiassin

1998

Acta Biotechnologica

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Different cultural parameters that regulate pectinolytic enzyme production in vitro by Trumetes trogii were studied. When grown in a medium containing pectin, T. trogii produced extracellular polymethylgalacturonase, polygalacturonase and pectin'lyase but no pectate lyase activity. No significant differences in the maximum enzyme activities measured were observed with the addition of xylan, carboxymethylcellulose or both to the medium containing pectin. The addition of glucose to that medium considerably decreased all the activities studied, and in a medium with glucose as the sole carbon source no galacturonase activity could be measured, and pectin lyase activity was at its minimum. The low synthesis of pectin lyase in cultures containing glucose suggests that this enzyme is constitutive in contrast to the polygalacturonases that were not detected. The increase in pectin concentration stimulated growth and enzyme production. The highest specific activities were attained with the greatest concentration tested (15 a). Casamino acids were the best nitrogen source for enzyme production. Maximum growth was measured at pH 3.3; pH values of around 4.5 stimulated enzyme production, but high pectinase activities were also detected in media with more alkaline initial pH values (6.2 for galacturonases and 6.6 for lyases), probably owing to the specific induction of particular isoforms. In the m g e of 23 to 28 OC, good results were obtained in growth as well as in enzyme production. The addition of Tween 80 promoted growth and gave the highest yield of polymethylgalacturonase and pectin lyase (0.37 and 36.2 E.UJm1, respectively). The highest polygalacturonase activity (1.1 E.UJml) was achieved with polyethylene glycol. Tween 20 and Triton X-100 inhibited growth and pectinase production.

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“…It is responsible for foot, crown and root rot in this crop and it is pathogenic on crops which are resistant to races 1 and 2 of Fusarium oxysporum f. sp. lycopersici [6] (FOL).…”

Section: (Forl) Wasmentioning

confidence: 99%

Purification and characterization of an exopolygalacturonase produced by Fusarium oxysporum f. sp. radicis lycopersici

VAZQUEZ¹

1993

FEMS Microbiology Letters

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An exopolygalacturonase produced by Fusarium oxysporum f. sp. radicis lycopersici, a fungus that produces root rot, was purified by gel filtration and ion exchange chromatography. It had a Mr 68 K, a pH optimum of 5.6 and an optimum temperature of 60°C. This polygalacturonase was inhibited by calcium ions and had a Km of 0.64 mM using sodium polypectate as substrate. The exo mode of action of this enzyme was revealed by thin‐layer chromatography of hydrolysed substrate.

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Kinetic of the production of polygalacturonase and pectin lyase by two closely relatedFormae speciales ofFusarium oxysporum

Cited by 14 publications

References 15 publications

**Morphological and biochemical characterization of Armillaria isolates from Zimbabwe**

**Morphological and biochemical characterization of Armillaria isolates from Zimbabwe**

Culture conditions for the production of pectinolytic enzymes by the white‐rot fungus Trametes trogii on a laboratory scale

Purification and characterization of an exopolygalacturonase produced by Fusarium oxysporum f. sp. radicis lycopersici

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