Kinetic modelling of enzymatic biodiesel production from castor oil: Temperature dependence of the Ping Pong parameters

Abstract

Biodiesel is considered an alternative replacement for petroleum diesel, and enzymatic reaction is one method for biodiesel production. Understanding the kinetics of this reaction is important to achieve higher production rates. The kinetics of transesterification of castor oil using Novozym 435 was investigated in this study. The genetic algorithm was employed for estimation of kinetic parameters including Vmax, KmTG, and KmA in the Ping Pong kinetic model at different temperatures. In most enzymatic reactions that follow the Ping Pong mechanism, inhibition by alcohol has been taken into consideration. Here, we examined a competitive inhibition mechanism and found no inhibition effect at high alcohol‐to‐oil molar ratios of 12.6, 15.6, and 18.9 mol/mol. Arrhenius expressions were used to fit the kinetic parameters for reaction temperatures of 30–50 °C. An acceptable agreement with experimental data at 45 °C was observed when biodiesel yields were predicted using the temperature‐dependent parameters, thus validating the proposed model.

Production of biodiesel catalyzed by lipase from Thermomyces lanuginosus in its soluble form

“…In an attempt to overcome these problems, most literature reports adopt immobilized lipases as a biocatalyst, allowing its posterior reutilization . Unfortunately, the most suitable supports for the immobilization process also present, to date, high prices, thus shifting the number of catalyst reuse cycles to search for a cost‐effective process .…”

“…[17] In an attempt to overcome these problems, most literature reports adopt immobilized lipases as a biocatalyst, allowing its posterior reutilization. [18][19][20] Unfortunately, the most suitable supports for the immobilization process also present, to date, high prices, thus shifting the number of catalyst reuse cycles to search for a cost-effective process. [21] In these circumstances, the use of lipases in aqueous formulations-with lower manufacturing costs than purified and supported (immobilized) enzymes-such as the novel lipase developed by Novozymes, Callera TM Trans L, arises as a promising alternative for the enzymatic biodiesel production.…”

Effect of light irradiation on esterification of oleic acid with ethanol catalyzed by immobilized Pseudomonas cepacia lipase

“…Many enzyme catalyzed esterification and transesterification reactions follow the Ping‐Pong Bi‐Bi mechanism . The schematic diagram illustrated in Figure represents the Ping‐Pong Bi‐Bi mechanism with competitive inhibition by both alcohol and acid substrates.…”

“…The estimated kinetic parameters and their comparisons under light and dark conditions are shown in Table . Based on the calculated kinetic parameters, K A was found to be lower than K B , indicating a higher affinity between the enzyme and oleic acid compared to enzyme to alcohol . Additionally, it can be seen that a significant change was observed in the values of the reaction parameters under light and dark conditions.…”

“…Many enzyme catalyzed esterification and transesterification reactions follow the Ping-Pong Bi-Bi mechanism. [17,18,21] The schematic diagram illustrated in Figure 6 represents the Ping-Pong Bi-Bi mechanism with competitive inhibition by both alcohol and acid substrates. As can be seen in Figure 6, the enzyme (E) active site attached with the acyl donor (A) forms an enzyme acyl complex (EA), which causes the water molecule to be released from the EA.…”