Effect of light irradiation on esterification of oleic acid with ethanol catalyzed by immobilized Pseudomonas cepacia lipase

Abstract

The present study demonstrates the effect of light irradiation on the esterification of oleic acid catalyzed by immobilized Pseudomonas cepacia lipase. The reaction rates of all the experiments under light irradiation were found to be higher than dark conditions. The kinetics of reactions supported the Ping‐Pong Bi‐Bi mechanism with dead end inhibition by both the alcohol and acid substrates. Moreover, circular dichroism (CD) spectroscopy was used to analyze the effect of light on lipase enzyme. The CD spectroscopic studies confirmed that the conformational changes in the secondary structure of the lipase enzyme increased the reaction rate of light‐illuminated experiments, which might have opened up the active sites of enzymes and thus, resulted in higher reaction rates compared to dark reactions. These results have successfully demonstrated that the light illumination positively influenced the rate of P. cepacia enzyme‐catalyzed esterification reactions.

Thermo‐responsive macroporous p(NIPAM) cryogel affords enhanced thermal stability and activity for ɑ‐glucosidase enzyme by entrapping in situ

“…[ 7–10 ] It has been reported that various matrices such as synthetic, organic, and biological polymers can be used as support materials for enzyme immobilization. [ 11–17 ] Materials recommended as support in many instances are not suitable for industrial processing procedures due to their low mechanical strength, unsuitable chemical functionality, and inappropriate physical conditions. [ 18–21 ]…”

“…[7][8][9][10] It has been reported that various matrices such as synthetic, organic, and biological polymers can be used as support materials for enzyme immobilization. [11][12][13][14][15][16][17] Materials recommended as support in many instances are not suitable for industrial processing procedures due to their low mechanical strength, unsuitable chemical functionality, and inappropriate physical conditions. [18][19][20][21] Three-dimensional (3-D) polymeric matrices with nano-, micro-, and macropore structures are desirable in many biomedical applications, including catalysis, drug delivery, and protection of proteins, enzymes, DNA, and other biomacromolecules.…”

Biocatalytic Approach for Direct Esterification of Ibuprofen with Sorbitol in Biphasic Media

“…Among the most commonly used non-immobilized (free) lipases for esterification or transesterification reactions are those from the microorganisms Rhizomucor miehei [ 36 ], Candida. rugosa [ 37 ], and Pseudomonas cepacian [ 38 ]. Immobilized lipases such as Novozyme 435 ( C. antarctica lipase B, CALB) [ 39 ], Lipozyme RM-IM (lipase from R. miehei ), and Lipozyme TL-IM (lipase from T. lanuginosus ) [ 40 ] are also commercially available.…”