Kinetic Growth of Saccharomyces cerevisiae in Non Dairy Creamer Wastewater Medium

Abstract

Efforts in non dairy creamer (NDC) wastewater treatment can be performed by converting it into raw materials, i.e. as a medium for microorganisms growth. This research had an objective of examining the growth kinetics of Saccharomyces cerevisiae in the fermentation of the non dairy creamer wastewater for protein production. Kinetic study of the growth and fermentation is necessary as an introduction to understanding each process of the fermentation. The research applied an Experimental Design with a full factorial design. Treatments during the experiment consisted of P1 (NDC medium concentration of 25%), P2 (NDC medium concentration of 50%), P3 (NDC medium concentration of 75%), and P4 (NDC medium concentration of 100%). The cellular concentrate intakes were 105, 106, 107, and 108 cell/ml. The experiment resulted in specific growth rate (µ) of 0.240868 (hour-1), maximum specific growth rate (µmax) of 55.7479 (hour-1), with the highest protein contents of 22.15 mg/l at the concentration medium of 75%. IntroductionNon dairy creamer wastewater that migrates from waste disposal has been proven to cause pollution due to inadequate treatment. In addition to reducing water body quality, the wastewater also has bad smell, which also impacts the affected environment. The smell comes from organic compounds within the wastewater. Therefore, proper treatment is important to convert the wastewater into valuable raw materials.There have been many studies concerning wastewater as a more affordable growth medium for microorganisms in order to produce single cell proteins. However, none of the studies has done so with non dairy creamer wastewater. The use of Saccharomyces cerevisiae in the treatment of non dairy creamer wastewater is expected to have a promising prospect in producing single cell proteins. Single cell proteins are dry cells, which can be found in khamir, bacteria, and algae. These biomass microorganisms are potential sources of proteins for human food and livestock feed. The single cell proteins can be an alternative to fulfill the future needs for proteins because they contain particular proteins as well as carbohydrates, fat, mineral, and other nutrients for both human and animals, cited from [1][2][3]. The use of Saccharomyces cerevisiae in the non dairy creamer wastewater treatment is expected to be effective in the production of the single cell proteins.According to laboratory analysis, the non dairy creamer wastewater contained organic matters, in particular carbon in the form of carbohydrate, protein, and fat, so that they have a great potential to be a growth medium for microorganisms. One of the treatments of the non dairy creamer industry wastewater can be converting the wastewater into raw materials. These raw materials are useful for the medium of the microorganism fermentation developed in the production of the single cell proteins.The fermentation process can be assessed through fermentation kinetics. Kinetics study of the growth and fermentation is important as an introduction to understand...

Effects of compost quality from non-dairy creamer sewage sludge-solid compost biogas-rice husk char on bok choy mustard (Brassica rapa L.)

“…Sewage sludge from non-dairy creamer industrial has high potential value as a source of organic matter and plant nutrition for soil properties improvement. The wastewater from non-dairy creamer production contains organic matter, especially carbon in the form of carbohydrates, proteins, fats, and lipid (Dewi et al, 2016a;2016b;Triyaswati and Ilmi, 2020) therefore sewage sludge from non-dairy creamer also contains high organic matter. Non-dairy creamer sewage sludge (NDCSS) is an important type of solid organic waste and NDCSS compost is basic material organic fertilizer.…”