Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique

Abstract

Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune system diseases in order to contribute to improved understanding and evaluation of binding parameters for a variety of interactions between antigens and antibodies biochemically and clinically. Since the binding constants determination of an anti-protein dengue antibody (Ab) to a protein dengue antigen (Ag) is mostly complicated, the SPR technique aids a determination of binding parameters directly for a variety of particular dengue Ag_Ab interactions in the real-time. The study highlights the doctrine of real-time dengue Ag_Ab interaction kinetics as well as to determine the binding parameters that is performed with SPR technique. In addition, this article presents a precise prediction as a reference curve for determination of dengue sample concentration.

Abrupt phase change in graphene-gold spr-based biosensor

“…In the experimental measurements, before injection liquid samples inside the chamber the phase changes of the chamber must be determined. Usually for the study of evolution of phase change, the phase sensitivity of the system is calculated via the conventional relationship in equation (4). In this equation the sensitivity is found through a maximum value of phase difference between two consecutive phase spectrum.…”

“…Sensors based on SPR are powerful tools for real-time supervising of interactions in medicine, biology and chemistry analysis due to the enhancement of the surface sensitivity and accurate detection of the sensing medium molecules' reaction [1][2][3][4]. Surface plasmons (SPs) are collective oscillations of free electrons at the metal-dielectric interface [5].…”

Recent Trends in Dengue Detection Methods Using Biosensors

“…Overall, the use of an electronic system supporting the optoelectronic components is required to allow for data processing for both types of optical biosensors. Currently, a lot of research has been done in the exploitation of dengue optical biosensors [46][47][48]. Figure 11 depicts a study on 10-minutes of early detection for all four dengue virus serotypes via surface plasmon resonance (SPR) technique [46].…”

“…To date, this group has been continued their projects on kinetic analysis of IgM monoclonal antibodies binding to DENV-2 antigens using the SPR technique [48]. In these projects, prior to dengue sample injection, a chip of gold sensor surface was initially activated with ___________________________________________________________________________ 147 immobilization of carboxymethyl-dextran matrix as a linker layer, N-hydroxysuccinimide (NHS) and N-ethyl-N-(dimethylaminopropyl) carbodiimide (EDC).…”

Surface Plasmon Resonance: A Boon for Viral Diagnostics

“…SPR-based food monitoring includes detection of antibiotics in milk samples (using a portable six-channel SPR sensor) and mycotoxin patulin (immunochemical SPR) (Hunt and Armani, 2010;Damborský et al, 2016). SPR was also utilized to monitor the binding kinetics of dengue antibody with corresponding dengue antigen in real-time manner (Jahanshahi et al, 2016).…”