Kinesin motor KIFC1 is required for tubulin acetylation and actin-dependent spindle migration in mouse oocyte meiosis

Abstract

Mammalian oocyte maturation is a unique asymmetric division, which is mainly due to actin-based spindle migration to the cortex. In present study, we reported that a kinesin motor KIFC1, which was associated with microtubules for the maintenance of spindle poles in mitosis, was also involved into actin dynamics in oocyte meiosis. KIFC1 co-localized with microtubules during mouse oocyte maturation. Depletion of KIFC1 caused the failure of polar body extrusion, and we found that meiotic spindle formation and chromosome alignment were disrupted. This might be due to the effects of KIFC1 on HDAC6 and NAT10-based tubulin acetylation, which further affected microtubule stability. Mass spectroscopy analysis revealed that KIFC1 also associated with several actin nucleation factors and we found that KIFC1 was essential for the distribution of actin filaments, which further affected spindle migration. Depletion of KIFC1 leaded to aberrant expression of Formin2 and ARP2/3 complex, and ER distribution was also disturbed. Exogenous KIFC1 mRNA supplement could rescue these defects. Taken together, besides its roles on tubulin acetylation, our study also reported a novel role of kinesin KIFC1 on the regulation of actin dynamics for spindle migration in mouse oocytes.

KAT8 functions in redox homeostasis and mitochondrial dynamics during mouse oocyte meiosis progression

“…Microtubule stability depends on acetylated tubulin level. 6 Our results indicated that the expression level of acetylated tubulin was changed in Kat8-OE oocytes. studies indicated that reduced tubulin affected stability, in spite of this, high lin acetylation did not stabilize microtubules.…”

“…Microtubule stability depends on acetylated tubulin level 6 . Our results indicated that the expression level of acetylated tubulin was changed in Kat8 ‐OE oocytes.…”

“…Any error in this process causes embryonic developmental arrest and implantation failure, resulting in spontaneous abortion of the fetus or the birth of chromosomally unbalanced offspring 5 . Several lines of evidence support the viewpoint that redox homeostasis plays a core role in proper spindle assembly and chromosome alignment during oocyte development, 6,7 but the potential molecules and pathways controlling meiosis are yet to be elucidated.…”

Ceria Nanoparticle Systems Alleviate Degenerative Changes in Mouse Postovulatory Aging Oocytes by Reducing Oxidative Stress and Improving Mitochondrial Functions

“…Mammalian oocyte maturation involves unique asymmetric division primarily driven by actin-based spindle migration to the cortex . As shown in Figure A, in control oocytes, filamentous actin (F-actin) were well-aggregated in the cortical region with robust structural integrity, and particularly more F-actin signal was labeled in the local cytomembrane adjacent to the chromosomes (Figure D).…”

Proteomic Profiling Reveals the Molecular Control of Oocyte Maturation

“…S3 B ). Of note, KIFC1, a kinesin family member, was recently demonstrated to be essential for microtubule stability in mouse oocytes and can rescue spindle instability in human oocytes ( 25 , 26 ). The analysis discovered the involvement of cell cycle–related proteins in meiotic maturation, and among them CEP57L1 was selected for further functional investigation.…”