Kinematic and head morphometric characterisation of spermatozoa from the Brown Caiman (Caiman crocodilus fuscus)

Valverde, Anthony; Madrigal‐Valverde, Mónica; Castro-Morales, Olivier; Gadea-Rivas, Arnoldo; Johnston, Stephen D.; Soler, Carles

doi:10.1016/j.anireprosci.2019.06.011

Cited by 18 publications

(20 citation statements)

References 57 publications

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“…The multivariate approach of PCs and cluster analysis conducted for several species underscores that ejaculates are nevertheless heterogeneous in that they contain spermatozoa with different motility and kinematic patterns [ 17 , 22 , 31 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. In the present study, we identified four different sperm SPs described from three PCs that represented velocity, progressiveness, and cell undulatory movement.…”

Section: Discussionmentioning

confidence: 99%

Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches

Víquez

Barquero

Soler

et al. 2020

Biology

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The ejaculate is heterogenous and sperm sub-populations with different kinematic patterns can be identified in various species. Nevertheless, although these sub-populations are statistically well defined, the statistical differences are not always relevant. The aim of the present study was to characterize kinematic sub-populations in sperm from two bovine species, and diluted with different commercial extenders, and to determine the statistical relevance of sub-populations through Bayesian analysis. Semen from 10 bulls was evaluated after thawing. An ISAS®v1 computer-assisted sperm analysis (CASA)-Mot system was employed with an image acquisition rate of 50 Hz and ISAS®D4C20 counting chambers. Sub-populations of motile spermatozoa were characterized using multivariate procedures such as principal components (PCs) analysis and clustering methods (k-means model). Four different sperm sub-populations were identified from three PCs that involved progressiveness, velocity, and cell undulatory movement. The proportions of the different sperm sub-populations varied with the extender used and in the two species. Despite a statistical difference (p < 0.05) between extenders, the Bayesian analysis confirmed that only one of them (Triladyl®) presented relevant differences in kinematic patterns when compared with Tris-EY and OptiXcell®. Extenders differed in the proportion of sperm cells in each of the kinematic sub-populations. Similar patterns were identified in Bos taurus and Bos indicus. Bayesian results indicate that sub-populations SP1, SP2, and SP3 were different for PC criteria and these differences were relevant. For velocity, linearity, and progressiveness, the SP4 did not show a relevant difference regarding the other sperm sub-populations. The classical approach of clustering or sperm subpopulation thus may not have a direct biological meaning. Therefore, the biological relevance of sperm sub-populations needs to be reevaluated.

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Section: Discussionmentioning

confidence: 99%

Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches

Víquez

Barquero

Soler

et al. 2020

Biology

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“…Differences in sperm morphometry have been described between various related species (Soler et al, 2014b;Cucho et al, 2016;Valverde et al, 2019c), subspecies (Beletti et al, 2005) and breeds (Saravia et al, 2007;Valverde et al, 2016) (Table 2). Extrinsic factors may include those which influence the environment of the donor (Immler et al, 2010;Yániz et al, 2015).…”

Section: Morphometrymentioning

confidence: 96%

The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review

Valverde¹,

Barquero²,

Soler³

2020

J. Anim. Feed Sci.

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“…Semen collection has been performed post-mortem in the crocodile [10]. Interest in the development of assisted breeding technology of captive animals [11] has led to a refinement of semen collection protocols and characterization of sperm variables [12][13][14][15]. A common method of semen collection in wildlife is electroejaculation but this approach is highly stressful for crocodiles and induces urine contamination [13].…”

Section: Introductionmentioning

confidence: 99%

“…Characterization of sperm subpopulations has also received attention in fishes [37][38][39]. A previous study in the brown caiman (Caiman crocodilus fuscus) has revealed that kinematic sperm subpopulations can also be identified in this species [15].…”

Section: Introductionmentioning

confidence: 99%

Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

et al. 2021

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There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P < 0.05). Principal component (PC) analysis revealed that kinematic variables grouped into three components: PC1, related to velocity; PC2 to progressiveness and PC3 to oscillation. Subpopulation structure analysis identified four groups (P < 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, and 31.3% of the total sperm population. Males differed in the proportion of sperm in each of the kinematic subpopulations. This new approach for the analysis of reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species.

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Kinematic and head morphometric characterisation of spermatozoa from the Brown Caiman (Caiman crocodilus fuscus)

Abstract: Kinematic and head morphometric characterisation of spermatozoa from the Brown Caiman (Caiman crocodilus fuscus)

Cited by 18 publications

References 57 publications

Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches

Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches

The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review

Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

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