Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

Valverde, Anthony; Castro-Morales, Olivier; Madrigal‐Valverde, Mónica; Camacho, Marlen; Barquero, Vinicio; Soler, Carles; Roldán, Eduardo R. S.

doi:10.1371/journal.pone.0248270

Cited by 4 publications

(2 citation statements)

References 56 publications

(79 reference statements)

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“…Several studies have described the presence of different sub-populations within an ejaculate [11,19,[61][62][63][64][65][66][67]. These sub-populations may be affected by external factors associated with semen, such as extender type or species [68,69]. Even the sperm sub-population distribution can vary depending on the statistical multivariate procedure used [70].…”

Section: Discussionmentioning

confidence: 99%

Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Barquero

Roldán

Soler

et al. 2021

Animals

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The aim of the study was to compare the morphometric features of sperm head size and shape from the Pietrain line and the Duroc × Pietrain boar crossbred terminal lines, and to evaluate their relationship with reproductive success after artificial insemination of sows produced from crossbreeding the York, Landrace and Pietrain breeds. Semen samples were collected from 11 sexually mature boars. Only ejaculates with greater than 70% motility rate and <15% of abnormal sperm were used for artificial inseminations (AI) and included in the study. Samples were analyzed using an ISAS®v1 computer-assisted sperm analysis system for eight morphometric parameters of head shape and size (CASA-Morph). Sub-populations of morphometric ejaculates were characterized using multivariate procedures, such as principal component (PC) analysis and clustering methods (k-means model). Four different ejaculate sub-populations were identified from two PCs that involved the head shape and size of the spermatozoa. The discriminant ability of the different morphometric sperm variables to predict sow litter size was analyzed using a receiver operating characteristics (ROC) curve analysis. Sperm head length, ellipticity, elongation, and regularity showed significant predictive capacity on litter size (0.59, 0.59, 0.60, and 0.56 area under curve (AUC), respectively). The morphometric sperm sub-populations were not related to sow litter size.

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Section: Discussionmentioning

confidence: 99%

Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Barquero

Roldán

Soler

et al. 2021

Animals

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“…This is the case for crocodile sperm cells. The new approaches to analyzing reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA systems technology, open up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species (132).…”

Section: General Recommendationsmentioning

confidence: 99%

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

Comizzoli

Holt

2022

Annu. Rev. Anim. Biosci.

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There is a remarkable diversity in the animal kingdom regarding mechanisms underlying the production, maturation, structure, and function of sperm cells. Spermatology studies contribute to the knowledge of species diversity and also provide information about individual or population fitness. Furthermore, this fundamental research is required before collected spermatozoa can be used for conservation breeding, including assisted reproduction and cryobanking. This article aims to ( a) review the most recent knowledge on sperm morphology and function in wild animal species, ( b) analyze how this knowledge can be used to save species in their natural habitat or ex situ, and ( c) propose future scientific directions in wildlife spermatology that could positively impact animal conservation. Variations in sperm structure and performance within and between species have multiple origins and significance. This collective body of knowledge enables the design and implementation of conservation strategies and action plans that integrate several disciplines. Expected final online publication date for the Annual Review of Animal Biosciences, Volume 10 is February 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Exposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus aurata

Oliveira,

Ferrão,

Gallego

et al. 2023

Fish Physiol Biochem

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Marine pollution by nanoparticles (NPs) can be reprotoxic for fish and disturb successful reproduction of wild populations. In gilthead seabream (Sparus aurata), a mild effect on sperm motility was observed after exposure to high concentrations of silver NPs. Considering the great heterogeneity traits within a sperm sample, it is possible that NPs affect spermatozoa accordingly, modulating subpopulation profile. Thus, this work aimed to analyse NP effects in sperm motility in general and considering spermatozoa population structure, using a subpopulation approach. Seabream sperm samples from mature males were exposed for 1 h to increasing concentrations of titanium dioxide (1, 10, 100, 1000 and 10,000 μg L−1) and silver (0.25, 25 and 250 μg L−1) NPs, including Ag NP and Ag+, dissolved in a non-activating medium (0.9 % NaCl). Concentrations chosen include realistic (10–100 and 0.25 μg L−1, respectively, for TiO2 and Ag) and supra-environmental values. The mean particle diameter was determined as 19.34 ± 6.72 and 21.50 ± 8.27 nm in the stock suspension, respectively, for titanium dioxide and silver. After the ex vivo exposure, sperm motility parameters were determined using computer-assisted sperm analysis, and sperm subpopulations were later identified using a two-step cluster analysis. Results revealed a significant reduction in total motility after exposure to the 2 highest concentrations of titanium dioxide NPs, while curvilinear and straight-line velocities were not altered. Exposure to silver NPs (Ag NP and Ag+) lowered significantly total and progressive motilities at all concentrations, while curvilinear and straight-line velocities were significantly lower only at the highest concentration. Sperm subpopulations were also affected by the exposure to both titanium dioxide and silver NPs. In both cases, the highest levels of NPs triggered a decrease in the percentage of fast sperm subpopulations (38.2% in TiO2 1000 μg L−1, 34.8.% in Ag NP 250 μg L−1, and 45.0% in Ag+ 250 μg L−1 vs 53.4% in the control), while an increase on slow sperm subpopulations. A reprotoxic effect was proven for both NPs, but only at supra-environmental concentrations.

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Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

Cited by 4 publications

References 56 publications

Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

Exposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus aurata

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