Killing of cutaneous microbial species by photodynamic therapy

Zeina, B.; Greenman, John; Purcell, Wendy M.; Das, Bikash

doi:10.1046/j.1365-2133.2001.04013.x

Cited by 238 publications

(227 citation statements)

References 23 publications

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“…42 In contrast to bacterial species, C. albicans sensitized by 100 mg/L MB was killed by visible light from a slide projector (42 mW/cm 2 ) after only 20 minutes of irradiation. 34 Giroldo et al 43 verified inhibition of C. albicans CFUs to approximately 50% in the presence of 50 mg/L MB and laser light irradiation of 28 J/cm 2 . These authors also verified that cell death promoted by this combination can be related to damage to the plasma membrane of the yeast.…”

Section: Discussionmentioning

confidence: 98%

“…Although yeasts are more difficult to kill by PDT than bacteria, 22,34 several in vitro investigations have demonstrated the photoinactivation of C. albicans. [17][18][19][20][21][22][23][24] The results of the present investigation demonstrated significant reduction of CFU/mL of C. albicans from tongues of mice after 400, 500, and 1000 mg/L of Photogem associated with LED light (305 J/cm 2 ) (455 and 630 nm).…”

Section: Discussionmentioning

confidence: 99%

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Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

View full text Add to dashboard Cite

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“…For this reason, there has been an increased interest in developing PDT for local infections treatment. Different types of PS have been proposed in laboratory investigations, including porphyrins [14,15], phenothiazine dyes [16,17], chlorins [18,19], and phthalocyanines [20]. However, complete killing of the microorganisms is not frequently achieved and some of the available PS showed limited effectiveness against Candida biofilms and animal models of candidiasis [15,21].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

et al. 2011

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Background and ObjectiveThe resistance of Candida species to antifungals represents a major challenge for therapeutic and prophylactic strategies. This study evaluated photodynamic therapy (PDT) mediated by Curcumin (CUR) against clinical isolates of C. albicans, C. tropicalis, and C. glabrata, both in planktonic and biofilm forms.Study Design/Materials and MethodsSuspensions of Candida were treated with three CUR concentrations and exposed to four LED fluences. The protocol that showed the best outcomes for inactivation of the planktonic phase was selected to be evaluated against Candida biofilms. In addition, two higher CUR concentrations were tested. The metabolic activity of biofilms was evaluated by means of XTT reduction assay and the biofilm biomass was evaluated using crystal violet (CV) staining assay. Data were analyzed in a mixed model nested ANOVA, Wilcoxon's nonparametric tests, and the Kruskal–Wallis test (α = 5%).ResultsThe use of CUR in association with light was able to promote a significant antifungal effect against the planktonic form of the yeasts. When using 40 µM of CUR, the metabolic activity of C. albicans, C. glabrata, and C. tropicalis biofilms was reduced by 85%, 85%, and 73%, respectively, at 18 J/cm2. CUR‐mediated PDT also decreased the biofilm biomass of all species evaluated. In addition, CV staining showed that C. albicans isolates were strong biofilm‐forming strains, when compared with C. glabrata and C. tropicalis isolates.ConclusionThe results from the present investigation showed that low CUR concentrations can be highly effective for inactivating Candida isolates when associated with light excitation. Lasers Surg. Med. 43:927‐934, 2011. © 2011 Wiley Periodicals, Inc.

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“…This could be explained by the poor absorption of the substances and the more complex targets present in a thick external wall of the yeast [5]. According to the literature, a multi-hit process is necessary in eukaryotes, whereby saturation of more than one molecular target is required before cell death occurs [23,24].…”

Section: Discussionmentioning

confidence: 99%

Visible light enhances the antimicrobial effect of some essential oils

Marqués-Calvo

Codony²,

Agustí³

et al. 2017

Photodiagnosis and Photodynamic Therapy

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The photodisinfection is a topical, broad spectrum antimicrobial technology, targeting bacteria, virus, fungi, and protozoa effective for single cells as for biofilms. Natural molecules have been studied less than synthetic agents in the process but they are currently receiving great interest. Therefore, the aim of this study is to evaluate for the first time if non-coherent blue and red light enhances the antimicrobial activity of some essential oils when standard strains for antibiotic or fungicide tests are enlightened in vitro. Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans collection strains were irradiated with monochromatic visible light from light emitting diodes in the presence of 5% and 0.5% eucalyptus (Eucalyptus globulus), clove (Eugenia caryophyllata), and thyme (Thymus vulgaris) essential oils. Microbial levels were measured by plate count on culture media. In this preliminary report, the results differ according to the kind and concentration of antimicrobial oils, the wavelength of light, and the prokaryotic or eukaryotic microorganism. The results support the idea that mainly blue light enhances the innate antimicrobial activity of the essential oils, especially phenols, and could offer a very efficient and natural way to combat microorganisms in several industries and medical applications.Postprint (author's final draft

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Killing of cutaneous microbial species by photodynamic therapy

Abstract: Overall, these results indicate that APDT of the skin may represent a useful alternative to conventional antimicrobial treatment.

Cited by 238 publications

References 23 publications

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

Visible light enhances the antimicrobial effect of some essential oils

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