KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light

Sarkisyan, Karen S.; Zlobovskaya, Olga A.; Gorbachev, D.A.; Bozhanova, Nina G.; Шаронов, Г. В.; Staroverov, Dmitry B; Egorov, Evgeny S.; Ryabova, Anastasia V.; Solntsev, Kyril M.; Mishin, Alexander S.; Lukyanov, Konstantin A.

doi:10.1371/journal.pone.0145287

Cited by 62 publications

(60 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar anti-inflammatory effect of IL-37 was revealed in a mouse model of AR. Kim et al [70] induced AR in mice by intraperitoneal injection of the HDM allergen, followed by intranasal provocation with the same allergen. As a result, the animals developed AR signs: an increased level of IgE, eosinophil infiltration of the nasal mucosa, and nasal hyperreactivity, which manifested itself in an increased frequency of sneezing.…”

Section: Reviewsmentioning

confidence: 99%

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

Shilovskiy¹,

Dyneva²,

Курбачева³

et al. 2019

Acta Naturae

View full text Add to dashboard Cite

Cytokines of the interleukin-1 (IL-1) family play an important role in the realization of the protective functions of innate immunity and are the key mediators involved in the pathogenesis of a wide range of diseases, including various manifestations of allergy. The IL-1 family includes more than 11 members. However, the functions of many of them remain to be elucidated. Recently, new members of the IL-1 family have been discovered. In 2000, several independent research groups reported the discovery of a new interleukin of this family, which was named IL-37, or IL-1F7 (according to the new nomenclature). IL-37 was assigned to the IL-1 family based on its structural similarity with other members of this family. The study of its biological properties showed that its activity changes in inflammatory diseases, such as rheumatoid arthritis, psoriasis, as well as allergic diseases (allergic rhinitis, bronchial asthma, and atopic dermatitis). However, unlike most members of the IL-1 family, IL-37 acts as a negative regulator of inflammation. Activation of IL-37 suppresses inflammation, resulting in the suppression of inflammatory cytokines and chemokines, which in turn prevents infiltration of pro-inflammatory cells, mainly eosinophils and neutrophils. The exact molecular and cellular mechanisms of the anti-inflammatory effect of IL-37 in the development of allergic diseases (AD) have not been fully studied. This review summarizes and analyzes the accumulated experimental data on the role of IL-37 in the pathogenesis of AD, such as allergic rhinitis, bronchial asthma, and atopic dermatitis.

show abstract

Section: Reviewsmentioning

confidence: 99%

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

Shilovskiy¹,

Dyneva²,

Курбачева³

et al. 2019

Acta Naturae

View full text Add to dashboard Cite

show abstract

“…The basic characteristics of KillerRed are listed in Table 1 (Bulina et al, 2006a,b; Lukyanov et al, 2010). Further mutations (G5C, Y68W, D119S, N147S, F179L, Y223H, E237Q) of KillerRed result in a blue-shifted KillerOrange , the photosensitization properties of KillerOrange remain to be investigated (Pletneva et al, 2015; Sarkisyan et al, 2015). …”

Section: Protein Photosensitizer For Nanoscopically-confined Photodynmentioning

confidence: 99%

Photodynamic Physiology—Photonanomanipulations in Cellular Physiology with Protein Photosensitizers

Jiang

Cui

2017

Front. Physiol.

View full text Add to dashboard Cite

Singlet oxygen generated in a type II photodynamic action, due to its limited lifetime (1 μs) and reactive distance (<10 nm), could regulate live cell function nanoscopically. The genetically-encoded protein photosensitizers (engineered fluorescent proteins such as KillerRed, TagRFP, and flavin-binding proteins such as miniSOG, Pp2FbFPL30M) could be expressed in a cell type- and/or subcellular organelle-specific manner for targeted protein photo-oxidative activation/desensitization. The newly emerged active illumination technique provides an additional level of specificity. Typical examples of photodynamic activation include permanent activation of G protein-coupled receptor CCK1 and photodynamic activation of ionic channel TRPA1. Protein photosensitizers have been used to photodynamically modulate major cellular functions (such as neurotransmitter release and gene transcription) and animal behavior. Protein photosensitizers are increasingly used in photon-driven nanomanipulation in cell physiology research.

show abstract

“…Light-inducible technologies have the potential to achieve targeted cell ablation in a more precise manner. Toward that goal, chromophores that generate reactive oxygen species (ROS) upon exposure to light have been applied in various systems (Buckley et al, 2017;Bulina et al, 2006;Makhijani et al, 2017;Qi et al, 2012;Sarkisyan et al, 2015;Xu and Chisholm, 2016).…”

Section: Introductionmentioning

confidence: 99%

Targeted cell ablation in zebrafish using optogenetic transcriptional control

Mruk

Ciepla

Piza

et al. 2019

Preprint

View full text Add to dashboard Cite

Cell ablation is a powerful method for elucidating the contributions of individual cell populations to embryonic development and tissue regeneration. Targeted cell loss in whole organisms has been typically achieved through expression of a cytotoxic or prodrug-activating gene product in the cell type of interest. This approach depends on the availability of tissue-specific promoters, and it does not allow further spatial selectivity within the promoter-defined region(s).To address this limitation, we have developed ablative methods that combine genetically encoded toxins, the tissue specificity afforded by cis-regulatory elements, and the conditionality of optogenetics. Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.

show abstract

KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light

Cited by 62 publications

References 40 publications

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

Photodynamic Physiology—Photonanomanipulations in Cellular Physiology with Protein Photosensitizers

Targeted cell ablation in zebrafish using optogenetic transcriptional control

Contact Info

Product

Resources

About