Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002

Marsh, Steven G.E.; Parham, Peter; Dupont, Bo; Geraghty, Daniel E.; Trowsdale, John; Middleton, Derek; Vilches, Carlos; Carrington, Mary; Witt, Campbell S.; Guethlein, Lisbeth A.; Shilling, Heather; García, Celsa; Hsu, Katharine C.; Wain, Hester

doi:10.1007/s00251-003-0571-z

Cited by 100 publications

(38 citation statements)

References 35 publications

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“…While 3DP1 was not typed in the SAC population, this pseudogene is considered a framework gene and should be present in all individuals, which is true for the other framework genes in the SAC. Haplotypes were then classified into either A or B, where group B haplotypes were defined as having one or more of the following genes present: 2DL5, 2DS1, 2DS2, 2DS3, 2DS5, and 3DS1 [13]. Conversely, group A haplotypes were defined based on the absence of all group B genes.…”

Section: Genotypingmentioning

confidence: 99%

Activating KIRs alter susceptibility to pulmonary tuberculosis in a South African population

et al. 2015

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Section: Genotypingmentioning

confidence: 99%

Activating KIRs alter susceptibility to pulmonary tuberculosis in a South African population

et al. 2015

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“…Certain assumptions were made while assigning genes to a specific haplotype which are as follows : i) the framework genes (KIR3DL3, 2DL4, 3DL2 and 3DP1) are present in all the haplotypes; ii) KIR3DL1 and 3DS1 are likely equivalent to alleles and iii) either KIR2DL2 or KIR2DL3 are present in a haplotype but not both. Conversely, the absence of all these genes define group A haplotypes [39]. Therefore, if any of the B haplotype genes are present, the genotype is considered as Bx.…”

Section: Kir Genotyping and Statistical Analysesmentioning

confidence: 99%

Study of genetic diversity of KIR and TLR in the Rabhas, an endogamous primitive tribe of India

et al. 2015

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“…This region, along with the Human Leukocyte Antigen (HLA) complex on chromosome 6p21.3, which encodes many known or presumed KIR ligands, ranks among the most polymorphic regions of the human genome (Campbell and Purdy, 2011). To date, 15 distinct KIR gene loci, plus two pseudogenes, have been defined (Marsh et al, 2003). KIR gene nomenclature reflects the structure of the receptor molecule encoded.…”

Section: Introductionmentioning

confidence: 99%

“…In general, “L” and “S” genes encode inhibitory and activating KIR, respectively (Middleton and Gonzelez, 2010). The final digit corresponds to the specific protein characterized within the group (numbered in order of their characterization) (Marsh et al, 2003). Analogous to HLA nomenclature, the KIR gene name is followed by a “*” separator followed by the numerical allele designation (Marsh et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The final digit corresponds to the specific protein characterized within the group (numbered in order of their characterization) (Marsh et al, 2003). Analogous to HLA nomenclature, the KIR gene name is followed by a “*” separator followed by the numerical allele designation (Marsh et al, 2003). KIR genes can vary from 4 to 16 Kb in length (genomic DNA) and encode four to nine exons encoding proteins 306–456 amino acids long (Selvakumar et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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A method for Killer-cell Immunoglobulin-like Receptor (KIR) 3DL1/3DS1 genotyping using DNA recovered from frozen plasma

Shahid

Chopera

Martin

et al. 2013

Journal of Immunological Methods

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We describe a reliable and semi-automated method for Killer-cell Immunoglobulin-like Receptor (KIR) 3DL1/S1 genotyping using DNA recovered from frozen plasma. The primers and protocol were first validated using two independent genomic DNA reference panels. To confirm the approach using plasma-derived DNA, total nucleic acids were extracted from 69 paired frozen PBMC and plasma specimens representing all common KIR3DL1/S1 genotypes (3DS1/3DS1, 3DS1/3DL1 and 3DL1/3DL1, including rare allele 3DL1*054), and analyzed in a blinded fashion. The method involves independent nested PCR amplification of KIR3DL1/S1 Exon 4, and if required Exon 3, using universal sequence-specific primers, followed by bidirectional sequencing. The free basecalling software RECall is recommended for rapid, semi-automated chromatogram analysis. KIR3DL1/S1 type assignment is based on two key nucleotide polymorphisms in Exon 4 and, if required, up to two additional polymorphisms in exon 3. Assignment can be performed manually or using our web-based algorithm, KIR3D. Extractions from plasma yielded median [IQR] nucleic acid concentrations of 0.9 [below the limit of detection-2.45] ng/μl. PCR was successful for 100% of exon 4 (69/69) and exon 3 (29/29) plasma amplifications. Chromatogram quality was high and concordance between PBMC and plasma-derived types was 100%. The estimated lower limit of input DNA required for reliable typing is 0.01 ng/μl. This method provides reliable and accurate KIR3DL1/S1 typing when conventional sources of high-quality genomic DNA are unavailable or limiting.

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Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002

Cited by 100 publications

References 35 publications

Activating KIRs alter susceptibility to pulmonary tuberculosis in a South African population

Activating KIRs alter susceptibility to pulmonary tuberculosis in a South African population

Study of genetic diversity of KIR and TLR in the Rabhas, an endogamous primitive tribe of India

A method for Killer-cell Immunoglobulin-like Receptor (KIR) 3DL1/3DS1 genotyping using DNA recovered from frozen plasma

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