Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR

Abstract

Renal interstitial fibrosis is a common pathological process in the progression of kidney disease. A nuclear magnetic resonance (NMR) based metabolomic approach was used to analyze the kidney tissues of rats with renal interstitial fibrosis (RIF), induced by unilateral ureteral obstruction (UUO). The combination of a variety of statistical methods were used to screen out 14 significantly changed potential metabolites, which are related with multiple biochemical processes including amino acid metabolism, adenine metabolism, energy metabolism, osmolyte change and induced oxidative stress. The exploration of the contralateral kidneys enhanced the understanding of the disease, which was also supported by serum biochemistry and kidney histopathology results. In addition, the pathological parameters (clinical chemistry, histological and immunohistochemistry results) were correlated with the significantly changed differential metabolites related with RIF. This study showed that targeted tissue metabolomic analysis can be used as a useful tool to understand the mechanism of the disease and provide a novel insight in the pathogenesis of RIF.

Uncovering the anticancer mechanism of Compound Kushen Injection against HCC by integrating quantitative analysis, network analysis and experimental validation

“…The typical 1 H - NMR spectra of cell and medium are present in Supplementary Figure S6 . Resonance assignments (Supplementary Table S1 ) were performed based on the chemical shifts of standard compounds from the Chenomx NMR suite, the Human Metabolome Database (HMDB) 18 , and Biological Magnetic Resonance Data Bank (BMRB) 19 , as well as the literature data 20 , 21 . The NMR spectra of cell and medium were dominated by peaks from amino acids, organic acids, choline-containing metabolites, and amine metabolites, in addition to some other metabolites.…”

1H NMR-Based Metabolomics Study of the Toxicological Effects in Rats Induced by “Renqing Mangjue” Pill, a Traditional Tibetan Medicine

“…Representative 600 MHz 1 H NMR spectra of plasma and tissue extracts obtained from control and HD on 15th days were showed in Figure 2 , with major metabolites in the integrated regions assigned. NMR spectral data was analyzed and specific metabolites were identified by the previously reported reference (Kim et al, 2014 ; Guo et al, 2015 ; Liao et al, 2016 ; Li et al, 2016 ) and in-house NMR database and the database of Chenomx NMR Suite 6.0 (Chenomx, Edmonton, Canada). Metabolites detected in plasma, tissue extracts including amino acids, glycolysis products, tricarboxylic acid cycle (TCA cycle) intermediates and choline metabolites, etc.…”

1H NMR studies on serum metabonomic changes over time in a kidney-Yang deficiency syndrome model

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