¹H NMR studies on serum metabonomic changes over time in a kidney-Yang deficiency syndrome model

Abstract

The central aim of this study was to investigate metabolite changes in metabolic pathwaysviametabonomic approaches in rats suffering from Kidney-Yang Deficiency Syndrome (KYDS) induced by hydrocortisone.

Integrated Systems Pharmacology, Urinary Metabonomics, and Quantitative Real-Time PCR Analysis to Uncover Targets and Metabolic Pathways of the You-Gui Pill in Treating Kidney-Yang Deficiency Syndrome

“…Studies have shown that under the interference of high doses of hydrocortisone, the hypothalamic monoamine transmitter in rats is activated and promotes the “over-consumption” of energy and immune system, which ultimately leads to a state of “exhaustion” similar to KYDS in TCM [5,11]. In addition, our previous study also found that the “exhaustion” of KYDS is mainly caused by damage to the mitochondria and liver of rats, thus affecting the body’s energy metabolism (TCA cycle and gluconeogenesis) [7,10]. On the contrary, a study has demonstrated that YGP exhibits anti-apoptosis effects (induced by exogenous glucocorticoid) via regulating mitochondrial function in apoptosis pathways of anterior pituitary cells [18].…”

“…It developed a significant decrease in the MOD group and increased in the YGP group. A myriad of studies have also shown that when KYDS occurs, amino acid metabolism is disrupted [5,7,10,13]. This indicates that various proteins and enzymes required for the synthesis of the body are inhibited, resulting in abnormal metabolism of life.…”

“…Studies have indicated that metabonomics based on different platforms (e.g., 1 H NMR [4,5,7,10], GC/MS [2,11], LC/MS [3,12,13,14,15,16,17]) can identify some biomarkers, targets, and metabolic pathways associated with KYDS or KYDS treated by Chinese medicine. However, it is unknown whether these corresponding targets and metabolic pathways can also be found via using metabonomics based on one platform (e.g., 1 H NMR) to study different biological samples of KYDS.At the same time, relevant reports on further molecular verification (e.g., RT-qPCR analysis) of these targets related to biomarkers and metabolic pathways have not yet, to our knowledge, been seen in KYDS’s research.…”

Combined systems pharmacology and fecal metabonomics to study the biomarkers and therapeutic mechanism of type 2 diabetic nephropathy treated with Astragalus and Leech

“…Creative Commons Attribution-NonCommercial 3.0 Unported Licence. gluconeogenesis pathway 84. Study has been proved that gluconeogenesis is closely related to the pathogenesis of DN, and ameliorate glucose metabolism by regulating gluconeogenesis is one of the important strategies against diabetic complications 69.…”

Holistic and dynamic metabolic alterations of traditional Chinese medicine syndrome in a toxic heat and blood stasis syndrome rat model

“…9 Qiu et al used time course plasma metabonomics analysis to investigate the material basis of the development of qi deciency/qi deciency and blood stasis syndromes in myocardial infarction induced heart failure rats. 3 Wang et al studied the mechanism of the hot syndrome through urine metabonomics, 10 and Chen et al investigated metabolite changes of serum samples through metabonomic approaches in a Kidney-Yang deciency syndrome rat model.…”