Kidney proteome responses in the teleost fish Paralichthys olivaceus indicate a putative immune response against Streptococcus parauberis

Cha, In-Seok; Kwon, Joseph; Park, Seong-Hwa; Nho, Seong Won; Jang, Ho Bin; Park, Seong-Bin; Castillo, Carmelo S. del; Hikima, Jun-ichi; Aoki, Takashi; Jung, Taehun

doi:10.1016/j.jprot.2012.05.046

Cited by 27 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, within the context of tumor diseases, Cdc48 p97/VCP alteration is linked to MHC class I presentation of some antigens (Ebstein et al, 2016). Interestingly, Cdc 48 has rarely been investigated in fish biology and one of the scarce studies showed downregulation of this protein in the teleost fish Paralichthys olivaceus after challenge with Streptococcus parauberis (Cha et al, 2012). His role for the maintenance of cellular function and the recovery of cellular damage after bacterial challenge in fish would deserve some investigations.…”

Section: Corticosteroids Do Not Appear As Strong Regulators Of Innate...mentioning

confidence: 99%

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Milla

Massart

Mathieu

et al. 2018

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

Section: Corticosteroids Do Not Appear As Strong Regulators Of Innate...mentioning

confidence: 99%

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Milla

Massart

Mathieu

et al. 2018

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

“…Kidneys, on the other hand, are the central immune and primary lymphoid tissues in teleost, secreting copious lymphocytes and macrophages [22]. Upon infection, they activate humoral and cellular immunity to resist pathogen invasion [23,24]. Simultaneously, prior studies have indicated that fish kidneys function analogously to mammalian bone marrow, promoting the production and maturation of erythrocytes and leukocytes [25].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Gene Networks in Three Components of Immune System Provides Novel Insights into Immune Response Mechanisms against Edwardsiella tarda Infection in Paralichthys olivaceus

Liu

Bao

et al. 2023

Animals

View full text Add to dashboard Cite

As a quintessential marine teleost, Paralichthys olivaceus demonstrates vulnerability to a range of pathogens. Long-term infection with Edwardsiella tarda significantly inhibits fish growth and even induces death. Gills, blood, and kidneys, pivotal components of the immune system in teleosts, elicit vital regulatory roles in immune response processes including immune cell differentiation, diseased cell clearance, and other immunity-related mechanisms. This study entailed infecting P. olivaceus with E. tarda for 48 h and examining transcriptome data from the three components at 0, 8, and 48 h post-infection employing weighted gene co-expression network analysis (WGCNA) and protein–protein interaction (PPI) network analysis. Network analyses revealed a series of immune response processes after infection and identified multiple key modules and key, core, and hub genes including xpo1, src, tlr13, stat1, and mefv. By innovatively amalgamating WGCNA and PPI network methodologies, our investigation facilitated an in-depth examination of immune response mechanisms within three significant P. olivaceus components post-E. tarda infection. Our results provided valuable genetic resources for understanding immunity in P. olivaceus immune-related components and assisted us in further exploring the molecular mechanisms of E. tarda infection in teleosts.

show abstract

“…In recent years, advances in mass-spectrometry (MS)-based approaches for proteomics have enabled us to investigate the mechanisms of the wing dimorphism of insects at proteomic levels. Isobaric tags for relative and absolute quantitation (iTRAQ) is an isobaric labeling approach combined with liquid chromatography and tandem mass spectrometry to identify and quantify proteins (Cha et al, 2012 ; Ren et al, 2016 ) and has been increasingly used in the past few years (Brewis and Brennan, 2010 ; Unwin, 2010 ). The objectives of this study are (1) to investigate the differential protein expression profiles between alate and apterous morphs of the pea aphid at different developmental stages and (2) to investigate the potential functions of chemoreception genes in wing dimorphism of aphids.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Comparative Proteomic Analysis Reveals Molecular Mechanisms Underlying Wing Dimorphism of the Pea Aphid, Acyrthosiphon pisum

Song¹,

Gao²,

Li³

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

Wing dimorphism is a widespread phenomenon in insects with an associated trade-off between flight capability and fecundity. Despite the molecular underpinnings of phenotypic plasticity that has already been elucidated, it is still not fully understood. In this study, we focused on the differential proteomics profiles between alate and apterous morphs of the pea aphid, Acyrthosiphon pisum at the fourth instar nymph and adult stages, using isobaric tags for relative and absolute quantitation (iTRAQ) in a proteomic-based approach. A total of 5,116 protein groups were identified and quantified in the three biological replicates, of which 836 were differentially expressed between alate and apterous morphs. A bioinformatics analysis of differentially expressed protein groups (DEPGs) was performed based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). KEGG enrichment analysis showed that DEPGs mainly participated in energy metabolism, amino acid biosynthesis and metabolism, and signal sensing and transduction. To verify the reliability of proteomics data, the transcriptional expression of 29 candidates of differentially expressed proteins were analyzed by quantitative real-time PCR (qRT-PCR), showing that 26 genes were consistent with those at proteomic levels. In addition, differentially expressed proteins between winged and wingless morphs that were linked to olfactory sense were investigated. Quantitative real-time PCR revealed the tissue- and morph-biased expression profiles. These results suggested that olfactory sense plays a key role in wing dimorphism of aphids. The comparative proteomic analysis between alate and apterous morphs of the pea aphid provides a novel insight into wing development and dimorphism in aphids and will help facilitate our understanding of these concepts at molecular levels.

show abstract

Kidney proteome responses in the teleost fish Paralichthys olivaceus indicate a putative immune response against Streptococcus parauberis

Cited by 27 publications

References 47 publications

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Investigation of Gene Networks in Three Components of Immune System Provides Novel Insights into Immune Response Mechanisms against Edwardsiella tarda Infection in Paralichthys olivaceus

iTRAQ-Based Comparative Proteomic Analysis Reveals Molecular Mechanisms Underlying Wing Dimorphism of the Pea Aphid, Acyrthosiphon pisum

Contact Info

Product

Resources

About