Ki-M8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage

Radzun, H. J.; Kreipe, H; Bodewadt, S; Ml, Hansmann; Barth, J; Mr, Parwaresch

doi:10.1182/blood.v69.5.1320.bloodjournal6951320

Cited by 12 publications

(17 citation statements)

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“…Another monocyte marker with potential predictive value for diabetic complications onset and progression is the Hp-Hb receptor CD163, a member of the scavenger receptor family. This CD marker is associated with anti-inflammatory activity and is exclusively expressed on the surface of monocyte/ macrophages [11][12][13][14][15][16]. High circulating levels of the soluble form of CD163 (sCD163) have been described in rheumatoid arthritis, liver disease, atherosclerosis, and obesity [14,[17][18][19][20], and increased sCD163 is associated with an increased risk for development of insulin resistance and type 2 diabetes [20].…”

Section: Introductionmentioning

confidence: 99%

Monocyte CD163 is altered in association with diabetic complications: possible protective role

Min

Brooks

Wong

et al. 2016

Journal of Leukocyte Biology

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The scavenger receptor CD163 is exclusively expressed by monocyte/macrophages and is shed by matrix metalloproteinases (MMPs) and neutrophil elastase (ELA2) as soluble CD163 (sCD163). Monocyte phenotype is altered in diabetes, but the relationship among monocyte CD163, sCD163, and diabetic complications is not known and was investigated in this study. Blood was obtained from patients with diabetes for >10 yr and mice with diabetes for ≤20 wk. Blood from people and mice without diabetes acted as controls. The percentage of CD163 monocytes and monocyte CD163 mRNA was determined by flow cytometry and qRT-PCR, respectively. Plasma sCD163, MMPs, and ELA2 were measured by ELISA. The ability of glucocorticoids to stimulate isolated monocyte CD163 expression was also investigated. The percentage of CD163 monocytes was significantly decreased and sCD163 significantly increased (both P < 0.05) in patients with diabetes with complications compared to those without complications. Plasma ELA2 and MMP-3 were also increased (P < 0.05), but CD163 mRNA was unaltered. sCD163 correlated with worsening renal function, as determined by eGFR (r = -0.48, P < 0.05). In diabetic mice, increased sCD163 at wk 5 and decreased percentage of CD163 monocytes at wk 10 preceded alteration in kidney collagen IV mRNA at wk 20 (all P < 0.05). In vitro incubation of monocytes in anti-inflammatory glucocorticoid increased the percentage of CD163 monocytes (P < 0.05). In people, higher sCD163 and decreased percentage of CD163 monocytes were consistent with increased monocyte activation and shedding. The murine data indicated that these changes preceded the development of diabetic complications. Taken together, these results suggest that higher circulating percentage of CD163 monocytes may have anti-inflammatory effects and may protect from development of diabetic complications.

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Section: Introductionmentioning

confidence: 99%

Monocyte CD163 is altered in association with diabetic complications: possible protective role

Min

Brooks

Wong

et al. 2016

Journal of Leukocyte Biology

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“…Ki-M8 has recently been reported to react with an intracytoplasmatically localized antigen in cells of the monocyte/macrophage system, including AM. 15 Our demonstration of a distinct surface reactivity of Ki-M8 antibodies in viable cells (Fig. 2) seems contradictory to this, but this apparent discordance cannot be explained.…”

Section: Discussionmentioning

confidence: 56%

Antigenic specificity of human alveolar macrophages and blood monocytes studied by an immunofluorescence technique

Nakstad

Lyberg

Baklien

et al. 1989

The Journal of Pathology

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Lung alveolar macrophages (AM) and blood monocytes (BM) are part of the mononuclear phagocyte system (MPS) and have been shown to be composed of several functionally and biochemically distinct subsets. AM and BM were each fractionated into four subfractions by centrifugation over Percoll, 95 per cent of the monocytes having a higher buoyant density than the high-density AM fraction. Antigen distribution within the cell subfractions was studied by an immunofluorescence technique using a panel of ten monoclonal antibodies (MoAbs) reacting with separate antigenic determinants previously found on mononuclear phagocytes. Several antigens were present on both types of cells (Ki-M1, Ki-M6, Leu-M3, Leu-M5, 1D5, Dako-Ma, and HLA-DR), while other antigens were expressed exclusively on BM (MAS 072 and MAS 081) or on AM (Ki-M8). Ki-M1, Leu-M3, Leu-M5, and HLA-DR were expressed to a greater degree on the surface of AM (95-96 per cent of cells) than on BM (56-68 per cent), while an inverse relationship was noted for 1D5 antigen (present on 41 per cent of BM and 11 per cent of AM). Ki-M6 and Dako-Ma MoAbs recognized an intracellularly restricted antigen present in both AM and BM. Ki-M1, Ki-M8, MAS 072, MAS 081, Leu-M3, Leu-M5, 1D5, and HLA-DR were expressed both in the cytoplasm and at the cell surface. Some antigens (1D5, Leu-M3, MAS 072, MAS 081, and Dako-Ma) showed an uneven distribution among subsets of both AM and BM.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…In order to stain cells of the monocyte/macrophage lineage we employed the monoclonal antibodies Ki M-1 to Ki M-8 (Behring, West Germany; Ki M-3 was a kind gift from Prof. M.R.Parwaresch, Kiel). These antibodies have been shown to react with different subsets of the mononuclear phagocyte system (Radzun 1986, Radzun et al 1987) and recognize antigens on lysosomes and cell membranes. Antibody binding was visualized by a modified immunoalkaline phosphatase method (Cordell et al 1984).…”

Section: Methodsmentioning

confidence: 99%

The spleen in Hodgkin's disease. An immunohistochemical study of lymphocyte subpopulations and macrophages

1988

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Lymphocyte subpopulations and macrophages in 16 spleens from patients with Hodgkin's disease were analysed immunohistochemically using monoclonal antibodies of the Leu and the Ki M series. In non-involved splenic tissue there is an increase of T-lymphocytes with an increased T-helper/T-suppressor cell ratio, while Ki M-1, -2, -3 and -5-positive, i.e. phenotypically different macrophages, are reduced. These results indicate that involvement of the spleen in Hodgkin's disease is accompanied by changes with respect not only to lymphocyte subpopulations but also to cells of the mononuclear phagocyte system. The immunodeficiency associated with Hodgkin's disease is probably not solely due to lymphocyte dysfunction, since the disease may lead, at least in the spleen, to alterations in macrophages and accessory cells and this may contribute to the impairment of cell-mediated immunity.

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Ki-M8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage

Cited by 12 publications

References 0 publications

Monocyte CD163 is altered in association with diabetic complications: possible protective role

Monocyte CD163 is altered in association with diabetic complications: possible protective role

Antigenic specificity of human alveolar macrophages and blood monocytes studied by an immunofluorescence technique

The spleen in Hodgkin's disease. An immunohistochemical study of lymphocyte subpopulations and macrophages

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