Khảo sát tình hình sử dụng thuốc và hóa chất trong nuôi tôm thẻ chân trắng (Litopenaeus vannamei) ở tỉnh Trà Vinh

Thịnh, Nguyễn Quốc; Maita, Masashi; Phú, Trần Minh

doi:10.22144/ctu.jsi.2020.040

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the number of farms using colistin was low, the high resistance to colistin was consistent with previous studies that performed antibiograms for V. parahaemolyticus isolates from seafood, clinical and environmental samples (e.g., Elexson et al, 2014; Obaidat et al, 2017; Sudha et al, 2014) and showed high resistance to colistin sulfate. In addition, our findings indicated that the use of antibiotics in prevention and treatment was different from the results reported in a previous study (Thinh et al, 2020), where farms mostly used amoxicillin (26.6%), followed by cotrim (20.0%), in the rearing of white leg shrimp in the Tra Vinh Province of the Mekong Delta. Our findings support a previous study (Liu et al, 2019) that identified tetracycline, quinolone, sulfadiazine, and erythromycin resistance genes in the intestinal bacteria of shrimp ( Penaeus vannamei ) by PCR, as well as 62 distinct ARGs (such as aac, macrolides, quinolones, tet, bla, and peptide antibiotics) by plasmid metagenomic analysis.…”

Section: Discussioncontrasting

confidence: 99%

Multi‐antibiotics resistance phenotype of pathogenic Vibrio parahaemolyticus isolated from acute hepatopancreatic necrosis disease in Litopenaeus vannamei farmed in the Mekong Delta

Thi

Thuy

et al. 2023

J World Aquaculture Soc

View full text Add to dashboard Cite

Vibrio parahaemolyticus is a significant causal agent of acute hepatopancreatic necrosis disease (AHPND), with huge production losses of white leg shrimp, Litopenaeus vannamei, cultivated globally, including aquaculture farms in the Mekong Delta of Vietnam. Controlling this disease is critical because of the worldwide expansion of antimicrobial‐resistant V. parahaemolyticus isolates. The purpose of this study was to determine the frequency of multi‐antibiotic resistance (MAR) in V. parahaemolyticus isolated from AHPND white leg shrimp extensively cultivated in Bac Lieu Province (Mekong Delta). Based on biochemical tests and toxR‐PCR positive detections, 34 V. parahaemolyticus isolates were identified. Antibiotic susceptibility examination revealed that most of the isolates were phenotypically multidrug resistant, including resistance to ceftazidime (100%) and amoxicillin (97.06%), followed by colistin (74%) and erythromycin (65%). These isolates were highly sensitive to doxycycline (94%), followed by florfenicol (74%) and flumequine (71%). There were 27 distinct MAR phenotypes detected among 34 isolates, with 14.71% of the isolates exhibiting the antibiotic profile AMO‐CEF‐CEP‐COL‐ERY, followed by 11.76% for each of the profiles AMO‐CEP‐TET‐COL and AMO–CEP–COL. In particular, 25 isolates (75%) were resistant to at least 5 (of the 12) antibiotics tested. A hierarchical clustering analysis of antibiotic‐resistant V. parahaemolyticus isolates indicated cross‐transmission across farms and its ability to survive in aquatic environments for extended periods (over 3 months). Our findings support the hypothesis that the variety of MARPs in V. parahaemolyticus is the result of human activities. Environmentally friendly therapy strategies should be used for the prophylaxis and treatment of V. parahaemolyticus infection.

show abstract