Key phases in the formation of caveolae

Parton, Robert G.; Tillu, Vikas A.; McMahon, Kerrie‐Ann; Collins, Brett

doi:10.1016/j.ceb.2021.01.009

Cited by 39 publications

(36 citation statements)

References 59 publications

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“…Importantly, Cavin1-positive dots detected in live cell imaging were almost always positive for Cav1 ( Fig. 2A , lower panel) indicating that they correspond to caveolae ( 20℃22 ), which is consistent with our EM data in fixed cells.…”

Section: Resultssupporting

confidence: 88%

“…1B and S1D ). The presence of Cavin1 dots suggested the existence of caveolae at the midbody, since Cavin1 at the plasma membrane is only present on fully budded caveolae at the plasma membrane ( 22 ). To determine whether bona fide caveolae were present at the midbody, we turned to electron microscopy (EM).…”

Section: Resultsmentioning

confidence: 99%

“…Here, we identified caveolae as a key regulator that limits ICB tension to promote abscission. Caveolae are characteristic cup-shaped 60-80 nm invaginations of the plasma membrane, discovered in the 1950’s ( 18–22 ). The formation of caveolae requires two types of structural components: Caveolins, which are partially embedded in the plasma membrane, and Cavins that form a coat around caveolae ( 20, 21 ).…”

Section: Introductionmentioning

confidence: 99%

“…The formation of caveolae requires two types of structural components: Caveolins, which are partially embedded in the plasma membrane, and Cavins that form a coat around caveolae ( 20, 21 ). In addition, accessory proteins such as EHD2 and PACSINS regulate caveolae dynamics ( 20℃22 ). In vertebrates, caveolae are particularly abundant in cells that experience chronical mechanical constraints, such as muscle cells, endothelial cells and adipocytes ( 20, 21 ).…”

Section: Introductionmentioning

confidence: 99%

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Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis

Andrade

Bai

Gupta

et al. 2022

Preprint

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During cytokinesis, the intercellular bridge (ICB) connecting the daughter cells experiences pulling forces, which delay abscission by preventing the assembly of the ESCRT scission machinery. Abscission is thus triggered by tension release, but how ICB tension is controlled is unknown. Here, we report that caveolae, which are known to control membrane tension upon mechanical stress in interphase cells, are located at the midbody, at the abscission site and at the ICB/cell interface in dividing cells. Functionally, the loss of caveolae delays ESCRT-III recruitment during cytokinesis and impairs abscission. This is the consequence of a 2-fold increase of ICB tension measured by laser ablation, associated with a local increase in myosin II activity at the ICB/cell interface. We thus propose that caveolae buffer membrane tension and limit contractibility at the ICB to promote ESCRT-III assembly and cytokinetic abscission. Altogether, this work reveals an unexpected connection between caveolae and the ESCRT machinery and the first role of caveolae in cell division.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis

Andrade

Bai

Gupta

et al. 2022

Preprint

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“…Interestingly, we found that LIN28A overexpression induces apoptosis in mIMCD‐3 cells in a Let‐7 independent manner. This apoptosis induction by Lin28A overexpression might be mediated, at least in part, by downregulation of Caveolin‐2 (CAV2), a member of the Caveolin family that is responsible for the caveolae formation (Parton et al., 2021) and also involved in signal transduction and other cellular processes (Bulacio et al., 2019; de Almeida, 2017; Sowa, 2011)…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induction by the stem cell factor LIN28A

Attali-Padael

Armon

Urbach

2021

Biology of the Cell

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Background information Lin28A and its paralog Lin28B are RNA binding proteins expressed in stem and progenitor cells, regulating the balance between their proliferation and differentiation. In‐vivo and in‐vitro experiments have shown that overexpression of these genes leads to abnormal cell proliferation, which results in many cases in cell transformation and tumor formation. Results Here we show, for the first time, that Lin28A overexpression can also lead to the opposite effect, i.e. apoptosis induction. We further demonstrate that this effect is specific to Lin28A but not to Lin28B and that it is mediated via the Let‐7 independent pathway in a complex mechanism that involves at least several proteins. Conclusions and Significance This unexpected observation suggests that cell fate regulation by Lin28 is dependent on a specific cellular/genetic context. Unraveling the cellular and molecular mechanisms underlying this Lin28A overexpression effect may pave the way for novel tumor therapeutic strategies, as Lin28 is commonly expressed in many types of tumors but not in most normal adult cells.

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Rafting on the Plasma Membrane: Lipid Rafts in Signaling and Disease

Isik

Cizmecioglu

2023

Advances in Experimental Medicine and Biology

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Key phases in the formation of caveolae

Cited by 39 publications

References 59 publications

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis

Apoptosis induction by the stem cell factor LIN28A

Rafting on the Plasma Membrane: Lipid Rafts in Signaling and Disease

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