Key genes associated with non-alcoholic fatty liver disease and acute myocardial infarction

Dai, Weiran; Sun, Yan; Jiang, Zhiyuan; Du, Kuan; Xia, Ning; Zhong, Guoqiang

doi:10.12659/msm.922492

Cited by 24 publications

(19 citation statements)

References 55 publications

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“…[ 58 , 59 ] Similarly, FPR2, JUN, PPBP, MMP9, TLR2, and FCER1G expression has significant diagnosis value in AMI patients and acts as potential targets for AMI-targeted therapy. [ 60 – 63 ] Consistent with these results, the present study indicated that IL1B, CXCL1, CXCL8, TNF, FPR2, JUN, PPBP, MMP9, TLR2, and FCER1G were upregulated hub DEGs.…”

Section: Discussionsupporting

confidence: 87%

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

Guo

Zhou

et al. 2021

Medicine

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Background: Acute myocardial infarction (AMI) is a common disease leading threat to human health around the world. Here we aimed to explore new biomarkers and potential therapeutic targets in AMI through adopting integrated bioinformatics tools. Methods: The gene expression Omnibus (GEO) database was used to obtain genes data of AMI and no-AMI whole blood. Furthermore, differentially expressed genes (DEGs) were screened using the “Limma” package in R 3.6.1 software. Functional and pathway enrichment analyses of DEGs were performed via “Bioconductor” and “GOplot” package in R 3.6.1 software. In order to screen hub DEGs, the STRING version 11.0 database, Cytoscape and molecular complex detection (MCODE) were applied. Correlation among the hub DEGs was evaluated using Pearson's correlation analysis. Results: By performing DEGs analysis, 289 upregulated and 62 downregulated DEGs were successfully identified from GSE66360, respectively. And they were mainly enriched in the terms of neutrophil activation, immune response, cytokine, nuclear factor kappa-B (NF-κB) signaling pathway, IL-17 signaling pathway, and tumor necrosis factor (TNF) signaling pathway. Based on the data of protein–protein interaction (PPI), the top 10 hub genes were ranked, including interleukin-8 (CXCL8), TNF, N-formyl peptide receptor 2 (FPR2), growth-regulated alpha protein (CXCL1), transcription factor AP-1 (JUN), interleukin-1 beta (IL1B), platelet basic protein (PPBP), matrix metalloproteinase-9 (MMP9), toll-like receptor 2 (TLR2), and high affinity immunoglobulin epsilon receptor subunit gamma (FCER1G). What's more, the results of correlation analysis demonstrated that there was positive correlation between the 10 hub DEGs. Conclusion: Ten DEGs were identified as potential candidate diagnostic biomarkers for patients with AMI in present study. However, further experiments are needed to confirm the functional pathways and hub genes associated with AMI.

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Section: Discussionsupporting

confidence: 87%

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

Guo

Zhou

et al. 2021

Medicine

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“…These genes were all downregulated after BS. The results were similar to previous studies [ 13 , 37 , 38 ]. IL-6 from adipose tissue, which travels through the portal vein to the liver, promotes NAFLD development [ 39 ].…”

Section: Discussionsupporting

confidence: 93%

“…By increasing the affinity of CXCR4, CD4 + T-cells deposition were increased in the livers of NAFLD patients [ 44 ]. Previous study had shown that FCER1G played a key role in the occurrence and progression of NAFLD [ 38 ]. Inhibition of SYK could ameliorate the inflammation and steatosis of NAFLD by reducing the activation of macrophages and the production of CCl2, IL-6 and TNF-α [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease

et al. 2021

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Increasing evidence shows that immune-related genes (IRGs) play an important role in bariatric surgery (BS). We identified differentially expressed immune-related genes (DEIRGs) of adipose tissue after BS by analysing the two expression profiles of GEO (GSE59034 and GSE29409). Subsequently, enrichment analysis, GSEA and PPI networks were examined to identify the hub IRGs and related pathways. The performance of the signature was evaluated by area under the curve (AUC) of the receiver operating characteristic (ROC). CIBERSORT algorithm was used to evaluate the relative abundance of infiltrated immune cells.42 DEIRGs were found between the GSE59034 and GSE29409 datasets. The AUC of the signature was 0.904 and 0.865 in the GSE58979 and GSE48452, respectively. Interestingly, the signature also showed good performance in diagnosing non-alcoholic fatty liver disease (NAFLD) (AUC was 0.834 and 0.800, respectively). The number of neutrophils, macrophages M2, macrophages M0 and dendritic cells activated decreased significantly. After BS, the infiltration of T cells regulatory, monocytes, mast cells resting and plasma cells in adipose tissue increased. The novel proposed IRGs signature reveals the underlying immune mechanism of BS and is a promising biomarker for distinguishing the severity of NAFLD. This will provide new insights into strategies for treating obesity and NAFLD.

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“…TYROBP, namely, Tyrosine kinase binding protein could activate NK cells through binding a variety of receptors ( 42 ). Studies showed that TYROBP could synthesize lipopolysaccharide and activated pro-inflammatory cytokines production ( 43 ). After TYROBP gene deletion, the pro-inflammatory cytokines decreased obviously, which indicated that TYROBP might be involved in NASH progression ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Key Genes and Pathways Involved in Nonalcoholic Steatohepatitis Improvement After Roux-en-Y Gastric Bypass Surgery

Zhou

et al. 2021

Front. Endocrinol.

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BackgroundAs the incidence of nonalcoholic fatty liver disease (NAFLD) increases globally, nonalcoholic steatohepatitis (NASH) has become the second common cause of liver transplantation for liver diseases. Recent evidence shows that Roux-en-Y gastric bypass (RYGB) surgery obviously alleviates NASH. However, the mechanism underlying RYGB induced NASH improvement is still elusive.MethodsWe obtained datasets, including hepatic gene expression data and histologic NASH status, at baseline and 1 year after RYGB surgery. Differentially expressed genes (DEGs) were identified comparing gene expression before and after RYGB surgery in each dataset. Common DEGs were obtained between both datasets and further subjected to functional and pathway enrichment analysis. Protein–protein interaction (PPI) network was constructed, and key modules and hub genes were also identified.ResultsIn the present study, GSE106737 and GSE83452 datasets were included. One hundred thirty common DEGs (29 up-regulated and 101 down-regulated) were identified between GSE106737 and GSE83452 datasets. KEGG analysis showed that mineral absorption, IL-17 signaling pathway, osteoclast differentiation, and TNF signaling pathway were significantly enriched. Based on the PPI network, IGF1, JUN, FOS, LDLR, TYROBP, DUSP1, CXCR4, ATF3, CXCL2, EGR1, SAA1, CTSS, and PPARA were identified as hub genes, and three functional modules were also extracted.ConclusionThis study identifies the global gene expression change in the liver of NASH patients before and after RYGB surgery in a bioinformatic method. Our findings will contribute to the understanding of molecular biological changes underlying NASH improvement after RYGB surgery.

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Key genes associated with non-alcoholic fatty liver disease and acute myocardial infarction

Cited by 24 publications

References 55 publications

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease

Integrated Analysis of Key Genes and Pathways Involved in Nonalcoholic Steatohepatitis Improvement After Roux-en-Y Gastric Bypass Surgery

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