Key enzymes catalyzing glycerol to 1,3-propanediol

Jiang, Wei; Wang, Shizhen; Wang, Qianqian; Fang, Baishan

doi:10.1186/s13068-016-0473-6

Cited by 77 publications

(64 citation statements)

References 154 publications

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“…In the glycerol anaerobic pathway, glycerol uptake facilitator protein, glycerol dehydrogenase, glycerol dehydratases with corresponding reactivation factors, and 1,3-propanediol oxidoreductase are the key enzymes for 1,3-PDO formation (31). The significant upregulation of these genes might guarantee the activities of corresponding enzymes for the 1,3-PDO production in crude glycerol in response to stresses, for which the upregulation of some was in agreement with the similar levels of corresponding enzyme activities detected in the crude glycerol cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Glycerol dehydratases encoded by three dhaB genes are regarded as key rate-limiting enzymes for catalyzing the dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) in the reductive pathway (31). The transcript levels of the three dhaB genes in K. pneumoniae 2e were all significantly upregulated in crude glycerol relative to that in pure glycerol, whereas the genes encoding propanediol dehydratase, which is regarded as the functional substitution of glycerol dehydratase, were all downregulated.…”

Section: Discussionmentioning

confidence: 99%

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Adaptability of Klebsiella pneumoniae 2e, a Newly Isolated 1,3-Propanediol-Producing Strain, to Crude Glycerol as Revealed by Genomic Profiling

Jiang

Hector

et al. 2019

Appl Environ Microbiol

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Crude glycerol is largely generated as the main by-product of the biodiesel industry and is unprofitable for industrial application without costly purification. The direct bioconversion of crude glycerol into 1,3-propanediol (1,3-PDO) by microorganisms is a promising alternative for effective and economic utilization. In this study, Klebsiella pneumoniae 2e was newly isolated for the conversion of crude glycerol into 1,3-PDO. Batch fermentation analysis confirmed that crude glycerol and its main impurities had slight impacts on the growth, key enzyme activity, and 1,3-PDO production of K. pneumoniae 2e. The 1,3-PDO yield from crude glycerol by K. pneumoniae 2e reached 0.64 mol 1,3-PDO/mol glycerol, which was higher than that by most reported 1,3-PDO-producing Klebsiella strains. Genomic profiling revealed that K. pneumoniae 2e possesses 30 genes involved in glycerol anaerobic metabolism and 1,3-PDO biosynthesis. Quantitative real-time PCR analysis of these genes showed that the majority of the genes encoding the key enzymes for glycerol metabolism and 1,3-PDO biosynthesis were significantly upregulated during culture in crude glycerol relative to that in pure glycerol. Further comparative genomic analysis revealed a novel glycerol uptake facilitator protein in K. pneumoniae 2e and a higher number of stress response proteins than in other Klebsiella strains. This work confirms the adaptability of a newly isolated 1,3-PDO-producing strain, K. pneumoniae 2e, to crude glycerol and provides insights into the molecular mechanisms involved in its crude glycerol tolerance, which is valuable for industrial 1,3-PDO production from crude glycerol. IMPORTANCE The rapid development of the biodiesel industry has led to tremendous crude glycerol generation. Due to the presence of complex impurities, crude glycerol has low value for industry without costly purification. Obtaining novel microorganisms capable of direct and efficient bioconversion of crude glycerol to value-added products has great economic potential for industrial application. In this work, we characterized a newly isolated strain, Klebsiella pneumoniae 2e, with the capacity to efficiently produce 1,3-propanediol (1,3-PDO) from crude glycerol and demonstrated its adaptation to crude glycerol. Our work provides insights into the molecular mechanisms of K. pneumoniae 2e adaptation to crude glycerol and the expression patterns of its genes involved in 1,3-PDO biosynthesis, which will contribute to the development of industrial 1,3-PDO production from crude glycerol.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adaptability of Klebsiella pneumoniae 2e, a Newly Isolated 1,3-Propanediol-Producing Strain, to Crude Glycerol as Revealed by Genomic Profiling

Jiang

Hector

et al. 2019

Appl Environ Microbiol

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“…[32] Until afew years ago, there were only chemical processes for the industrialp roduction of 1,3-PDO, starting from oil-based 1,2-EDOa nd acrolein. [35,36] 1,4-BDO is aw idely used chemical for polymers ynthesis, especially polyesters, polyamides,a nd polyurethanes, with an annualg lobal market of over 2.5 millions tons. [35,36] 1,4-BDO is aw idely used chemical for polymers ynthesis, especially polyesters, polyamides,a nd polyurethanes, with an annualg lobal market of over 2.5 millions tons.…”

Section: Main Biobased Aliphatic Polyolsmentioning

confidence: 99%

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

2018

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Biobased polymers have seen their attractiveness increase in recent decades thanks to the significant development of biorefineries to allow access to a wide variety of biobased building blocks. Polyesters are one of the best examples of the development of biobased polymers because most of them now have their monomers produced from renewable resources and are biodegradable. Currently, these polyesters are mainly produced by using traditional chemical catalysts and harsh conditions, but recently greener pathways with nontoxic enzymes as biocatalysts and mild conditions have shown great potential. Bacterial polyesters, such as poly(hydroxyalkanoate)s (PHA), are the best example of the biotic production of high molar mass polymers. PHAs display a wide variety of macromolecular architectures, which allow a large range of applications. The present contribution aims to provide an overview of recent progress in studies on biobased polyesters, especially those made from short building blocks, synthesized through step-growth polymerization. In addition, some important technical aspects of their syntheses through biotic or abiotic pathways have been detailed.

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“…As the name implies, the overexpression of genes is an abnormally and excessively high level of gene expression which produces a pronounced gene-related phenotype (46). The salient feature of overexpression is that the process can be carried out not only in the original cells where it exists (homologous host), but also in another expressing system (heterologous host).…”

Section: Overexpression Of Genesmentioning

confidence: 99%

“…When the byproduct pathways were deleted, the intracellular redox balance (NADH/NAD + ) was extremely high in these mutants, which has already been confirmed by some researchers. Therefore, these groups investigated further how to overexpress genes dhaB and dhaT to use these saved NADH (46,48).…”

Section: Overexpression Of Genes Associated With the Original Pathwaymentioning

confidence: 99%

Genetically Engineered Strains: Application and Advances for 1,3-Propanediol Production from Glycerol

Yang

Yun

Zhang

et al. 2017

Food Technol. Biotechnol.

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SUMMARY1,3-Propanediol (1,3-PD) is one of the most important chemicals widely used as monomers for synthesis of some commercially valuable products, including cosmetics, foods, lubricants and medicines. Although 1,3-PD can be synthesized both chemically and biosynthetically, the latter offers more merits over chemical approach as it is economically viable, environmentally friendly and easy to carry out. The biosynthesis of 1,3-PD can be done by transforming glycerol or other similar substrates using some bacteria, such as Clostridium butyricum and Klebsiella pneumoniae. However, these natural microorganisms pose some bottlenecks like low productivity and metabolite inhibition. To overcome these problems, recent research efforts have been focused more on the development of new strains by modifying the genome through different techniques, such as mutagenesis and genetic engineering. Genetically engineered strains obtained by various strategies cannot only gain higher yield than wild types, but also overcome some of the barriers in production by the latter. This review paper presents an overview on the recent advances in the technological approaches to develop genetically engineered microorganisms for efficient biosynthesis of 1,3-PD.

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Key enzymes catalyzing glycerol to 1,3-propanediol

Cited by 77 publications

References 154 publications

Adaptability of Klebsiella pneumoniae 2e, a Newly Isolated 1,3-Propanediol-Producing Strain, to Crude Glycerol as Revealed by Genomic Profiling

Adaptability of Klebsiella pneumoniae 2e, a Newly Isolated 1,3-Propanediol-Producing Strain, to Crude Glycerol as Revealed by Genomic Profiling

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

Genetically Engineered Strains: Application and Advances for 1,3-Propanediol Production from Glycerol

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