Keratinolytic activities of a new feather-degrading isolate of Bacillus cereus LAU 08 isolated from Nigerian soil

Lateef, Agbaje; Oloke, JK; Kana, E.B. Gueguim; Sobowale, B.O.; Ajao, S.O.; Bello, B.Y.

doi:10.1016/j.ibiod.2009.12.007

Cited by 59 publications

(38 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzyme activity reached a maximum of 50.4 U ml -1 at 72 h of cultivation, after which it decreased to a final value of 36.7 U ml -1 at 120 h, which is similar to KA reported for some bacilli [17,31].…”

Section: Bacterial Isolatesupporting

confidence: 86%

See 1 more Smart Citation

Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13

et al. 2014

Self Cite

View full text Add to dashboard Cite

In this study, crude extracellular keratinase obtained from a novel keratin-degrading bacterial strain, Bacillus safensis LAU 13 (GenBank accession No. KJ461434) was used for the synthesis of silver nanoparticles (AgNPs). The particles were characterised by UVVisible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy. The biosynthesised AgNPs exhibited maximum absorbance at 409 nm. They are spherical in shape with the size ranging 5-30 nm. The FTIR spectrum showed peaks at 3410, 2930, 1664, 1618, 1389 and 600 cm -1 , indicating that proteins were the capping and stabilisation molecules in the synthesis of AgNPs. Data obtained from XRD showed that the particles have face-centred cubic phase and are crystalline in nature with average size of *8.3 nm. The particles showed effective inhibitory activity against five clinical isolates of Escherichia coli. Therefore, the keratinase of this strain could be used to develop an environmental friendly method for the rapid synthesis of AgNPs. To the best of our knowledge, this is the first report of green synthesis of AgNPs using the metabolite of B. safensis, and the report adds to the growing relevance of B. safensis as a potential industrially viable organism.

show abstract

Section: Bacterial Isolatesupporting

confidence: 86%

“…The bacterium used in this study was isolated following the methods described by Lateef et al [17]. The medium used for isolation consisted of the following in g/L: NaNO 3 , 2; NaCl, 2; KH 2 PO 4 , 2; MgSO 4 , 0.05; FeSO 4 .7H 2 O, 0.1; CaCO 3 , 0.1; feather powder, 20; and agar-agar, 20 (BDH, UK).…”

Section: Microorganismmentioning

confidence: 99%

Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Feather degradation potential of Bacillus spp. has been widely reported in previous studies (Lateef et al 2010, Nilegaonkar et al 2007, Correa et al 2010). The time course study of feather degradation by strain PF1 reveals its potential to utilize feather keratin as a sole source of carbon and nitrogen.…”

Section: Discussionmentioning

confidence: 55%

“…High mechanical stability and rigid nature of keratin make them resistant to degradation by common proteolytic enzymes present in nature (Lateef et al 2010). Release of large amount of chicken feathers from poultry processing industries causes their accumulation (Tatineni et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Bhange

Chaturvedi

Bhatt

2016

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Background: Feathers are the major byproducts of poultry industry and considered as waste. Feathers (composed of protein keratin) are metabolized by a number of microorganisms as a source of carbon and nitrogen. Degradation of feathers results in production of amino acids and peptides, which can be employed as precursors for plant growthpromoting metabolites such as indole acetic acid, ammonia and HCN. The aim of the present investigation was to assess the influence of these metabolites (termed as feather protein hydrolysate) on plant growth promotion activity of keratinolytic bacterial strain Bacillus subtilis PF1.Results: Strain PF1 exhibits potent keratinolytic activity and can efficiently degrade 10 g/l chicken feathers under submerged cultivation with 81.4 ± 4.40 U/ml keratinase activity. Different concentrations of feathers supported the production of indole acetic acid by strain PF1. Strain PF1 produces maximum indole acetic acid (46.2 ± 0.21 µg/ml) in the presence of 2.0 % feathers at 120 h of incubation. The indole acetic acid production was confirmed by thin-layer chromatography and Fourier transform infrared spectroscopic analysis. However, increased concentration of feathers exhibited negative effect on phosphate solubilization due to increased alkalinity. HCN production also exhibited positive correlation with concentration of feathers. Finally, plant growth of Vigna radiata in the presence of strain PF1 with chicken feathers in soil was investigated, which showed good plant growth promotion activity. Increased ratio of C/N in soil also supported the plant growth promotion activity of feathers. Conclusion:Feather degradation property of B. subtilis PF1 could be efficiently utilized for feather waste management. The metabolites released by feather degradation along with strain PF1 could be successfully employed as an economic source of nitrogen fertilizers for plants.

show abstract

“…They use keratin as the sole source of C, N, S, and energy. Keratinolytic bacteria were most isolated from bird feathers and the plumage [54][55][56], composting [57], or feather waste processed by fermentation. The bacteria often belong to the genus Bacillus and order actinomycetes.…”

Section: Microbial Keratin Degradationmentioning

confidence: 99%

Keratinaceous Wastes and Their Valorization through Keratinolytic Microorganisms

Ningthoujam¹,

Tamreihao²,

SaikatMukherjee³

et al. 2018

Keratin

View full text Add to dashboard Cite

Keratin is a fibrous protein mainly found in higher vertebrates such as mammals, birds, and reptiles. It is also a major constituent of human epithelial tissues. Major keratinaceous wastes include skin, hair, wool, feather, horns, hooves, and nails. Large amounts of such wastes are generated from meat industry, poultry houses, and wool industry etc. Though keratinous wastes contain about 90% protein, keratin is usually recalcitrant to normal proteases. Such wastes have been traditionally digested using physico-chemical methods. But such techniques are energy-intensive and technologically demanding. Also, such approaches lead to degradation of certain amino acids such as lysine. In nature, keratinaceous wastes don't accumulate indicating that keratinolytic microorganisms exist in nature. Keratinase producing strains are distributed among bacteria, fungi, and actinobacteria etc. Hence, potent keratinolytic microbes and their enzymes may be used for valorization of keratinous wastes. Efficient degradation of such wastes may generate value-added products such as feed additives, agricultural biofertilizers, and cosmetics. This chapter will give a comprehensive overview of types of keratinaceous wastes, kinds of keratinolytic microbes and keratinases, and valorization of such wastes using keratinase producing strains and/or keratinases.

show abstract

Keratinolytic activities of a new feather-degrading isolate of Bacillus cereus LAU 08 isolated from Nigerian soil

Cited by 59 publications

References 15 publications

Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13

Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Keratinaceous Wastes and Their Valorization through Keratinolytic Microorganisms

Contact Info

Product

Resources

About