Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13

Lateef, Agbaje; Adelere, Isiaka Adedayo; Kana, E.B. Gueguim; Asafa, T. B.; Beukes, Lorika S.

doi:10.1007/s40089-014-0133-4

Cited by 150 publications

(95 citation statements)

References 38 publications

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“…6a), and this conforms with similar results earlier reported by several authors (Zaki et al 2011;Kannan et al 2013;Lateef et al 2015a). The particles were well dispersed, an indication of good stability to avoid aggregation.…”

Section: Biosynthesis and Characterization Of Agnpssupporting

confidence: 93%

“…The biosynthesized AgNPs displayed maximum absorbance at the wavelength of 436 nm (Fig. 2), which falls within the reported range of 391-440 nm for AgNPs (Thirumurugan et al 2011;Zaki et al 2011;Kannan et al 2013;Priyadarshini et al 2013;Lateef et al 2015a). It was observed that dilution ratio of 1:40 (extract:AgNO 3 solution), and pH 8.5 produced highest yield of AgNPs with maxima absorbance readings at 436 nm (Figs.…”

Section: Biosynthesis and Characterization Of Agnpssupporting

confidence: 82%

“…10 Antifungal activities of synthesized AgNPs on fungi inoculated into emulsion paint Appl Nanosci (2016Nanosci ( ) 6:863-874 871 et al 2004Nanosci ( , 2005Nanosci ( , 2006Nanosci ( , 2007Nanosci ( , 2010Lateef and Ojo 2015). The antibacterial activities of AgNPs as obtained in this study have been reported in previous studies (Priyadarshini et al 2013;Kanmani and Lim 2013;Salem et al 2014;Shankar et al 2014;Lateef et al 2015a). The antibacterial activities of AgNPs have been attributed to the interaction of AgNPs with sulphur and phosphorus containing biomolecules in the bacterial cell.…”

Section: Antimicrobial Activities Of Biosynthesized Agnpssupporting

confidence: 67%

“…About 0.1 g of cobwebs was weighed and hydrolyzed with 10 ml of 0.1 M NaOH at 90°C for 1 h. The hydrolyzed cobweb was allowed to cool and then centrifuged at 4000 rpm for 30 min. The supernatant obtained designated as cobweb extract was used to synthesize AgNPs as previously described (Lateef et al 2015a). About 1 ml of cobweb extract was added to the reaction vessel containing 40 ml of 1 mM silver nitrate (AgNO 3 ) solution for the reduction of silver ion.…”

Section: Green Synthesis and Characterization Of Agnpsmentioning

confidence: 99%

“…Several authors have reported variation in the color of AgNP colloidal solutions due to the composition of biomolecules responsible for the synthesis of the nanoparticles. Recently, we reported the formation of dark-brown AgNP solution using the crude extracellular keratinase of Bacillus safensis LAU 13 (Lateef et al 2015a). The biosynthesized AgNPs displayed maximum absorbance at the wavelength of 436 nm (Fig.…”

Section: Biosynthesis and Characterization Of Agnpsmentioning

confidence: 99%

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Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

et al. 2015

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In this study, spider cobweb as a novel biomaterial was used for the green synthesis of silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The efficacy of biosynthesized particles as antibacterial agents was evaluated using multidrug resistant clinical bacterial isolates through sensitivity testing with AgNPs and combination of AgNPs with some selected antibiotics. In addition, the potential application of the particles as additives in paints was demonstrated using some bacterial and fungal isolates. The synthesized AgNPs which were dark brown in color displayed maximum absorbance at the wavelength of 436 nm. It was observed that the reaction mixture of 1:40 (extract:AgNO 3 solution) at pH of 8.5 produced particles with maximum absorbance at 436 nm. The FTIR spectrum showed peaks at 3298, 2359, 2089, and 1635 cm -1 , indicating that proteins were the capping and stabilization molecules in the synthesis of AgNPs. The particles were spherical in shape with size ranging about 3-50 nm. The energy-dispersive X-ray analysis showed the presence of silver as the most prominent metal, while the selected area electron diffraction pattern conformed to the face-centered cubic phase and crystalline nature of AgNPs. The AgNPs inhibited the growth of several bacterial isolates including S. aureus, E. coli, Klebsiella granulomatis and P. aeruginosa in the range of 10-17 mm at concentration of 100 lg/ml. It was also demonstrated that AgNPs potentiated the activities of augmentin, ofloxacin and cefixime in the AgNP-antibiotic synergy studies. Similarly, the inclusion of AgNPs as additive in white emulsion paint led to the total inhibition of growth of E. coli, P. aeruginosa, Aspergillus niger and A. fumigatus. To the best of our knowledge, this is the first report of the use of cobweb for the green synthesis of AgNPs. The immense antimicrobial activities of the particles can be explored in the creation of novel products, where it can be used as additive to protect materials against microbial attack.

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Section: Biosynthesis and Characterization Of Agnpssupporting

confidence: 93%

Section: Biosynthesis and Characterization Of Agnpssupporting

confidence: 82%

Section: Antimicrobial Activities Of Biosynthesized Agnpssupporting

confidence: 67%

Section: Green Synthesis and Characterization Of Agnpsmentioning

confidence: 99%

Section: Biosynthesis and Characterization Of Agnpsmentioning

confidence: 99%

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Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

et al. 2015

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Current Advances in Biosynthesis of Silver Nanoparticles and Their Applications

Chokkareddy

Thondavada

Kabane

2018

Green Metal Nanoparticles

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Chemical characterization and anti‐breast cancer effects of silver nanoparticles using Phoenix dactylifera seed ethanolic extract on 7,12‐Dimethylbenz[a] anthracene‐induced mammary gland carcinogenesis in Sprague Dawley male rats

Mohammadi

Zangeneh

et al. 2019

Applied Organom Chemis

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The aim of the recent research was to investigate the anti‐breast cancer effects of silver nanoparticles using Phoenix dactylifera seed ethanolic extract (AgNPs). After preparation of Phoenix dactylifera seed extract, GC/MS was performed to detect the compounds. The findings indicated that 9‐Octadecenoic acid (Z)‐ methyl ester (40.95%) and Dodecanoic acid methyl ester (20%) were the most frequent constituents found in P. dactylifera. These nanoparticles were spherical with a size range of 17‐19 nm and characterized using various analytical techniques including UV–Vis absorption spectroscopy to determine the presence of AgNPs in the solution. We studied functional groups of P. dactylifera extract in the reduction and capping process of AgNPs by FT‐IR, crystallinity and FCC planes by X‐ray diffraction (XRD) pattern and surface morphology, shapes, and size of AgNPs by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). DPPH free radical scavenging test was used to test the antioxidant properties of P. dactylifera and AgNPs, which revealed high antioxidant potential similar to butylated hydroxy toluene (BHT) as the positive control. The results of cytotoxicity analysis indicated that P. dactylifera and AgNPs were toxic for MCF‐7 cells. In vivo design, induction of breast cancer was done by 7,12‐Dimethylbenz[a] anthracene (DMBA) in 50 animals. After 10 days, the animals were randomly divided into six subgroups, including healthy control, untreated control, two groups receiving the P. dactylifera at 2 and 6 mg/kg and two groups receiving the AgNPs at 2 and 6 mg/kg concentrations. Both doses of P. dactylifera and AgNPs (especially AgNPs6) significantly (p ≤ 0.05) reduced the weight and volume of liver, mammary gland, kidney, spleen, ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, creatinine, glucose, ferrous, ferritin, erythropoietin, GR, IL1, IL6, IL12, IL18, IFNY, and TNFα and increased HDL, total protein, albumin, WBC, lymphocyte, neutrophils, platelet, RBC, Hb, PCV, MCV, MCH, MCHC, SOD, CAT, GPx, IL4, IL5, IL10, IL13, and IFNα compared to the untreated group. Moreover, P. dactylifera and AgNPs (especially AgNPs6) significantly (p ≤ 0.05) treated breast cancer with reduction of organs free of metastasis compared to the untreated group. Seemingly, the AgNPs can be used for the treatment of breast cancer.

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Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13

Cited by 150 publications

References 38 publications

Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

Current Advances in Biosynthesis of Silver Nanoparticles and Their Applications

Chemical characterization and anti‐breast cancer effects of silver nanoparticles using Phoenix dactylifera seed ethanolic extract on 7,12‐Dimethylbenz[a] anthracene‐induced mammary gland carcinogenesis in Sprague Dawley male rats

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