Keratinocyte growth factor induced epithelial proliferation facilitates retroviral–mediated gene transfer to distal lung epithelia<i>in vivo</i>

Wang, Guoshun; Slepushkin, Vladimir; Bodner, Mordechai; Zabner, Joseph; Es, Helmuth H. G. van; Thomas, Patricia A.; Jolly, Doug J.; Davidson, Beverly L.; McCray, Paul B.

doi:10.1002/(sici)1521-2254(199901/02)1:1<22::aid-jgm1>3.0.co;2-x

Cited by 9 publications

References 37 publications

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Gene Delivery to the Airway

Keiser

Engelhardt

2013

CP Human Genetics

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This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of tracheal xenografts as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.

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Gene Delivery to the Airway

Keiser

Engelhardt

2013

CP Human Genetics

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Barrier Enhancing Signals in Pulmonary Edema

Birukov

Zebda

Birukova

2013

Comprehensive Physiology

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Increased endothelial permeability and reduction of alveolar liquid clearance capacity are two leading pathogenic mechanisms of pulmonary edema, which is a major complication of acute lung injury, severe pneumonia, and acute respiratory distress syndrome, the pathologies characterized by unacceptably high rates of morbidity and mortality. Besides the success in protective ventilation strategies, no efficient pharmacological approaches exist to treat this devastating condition. Understanding of fundamental mechanisms involved in regulation of endothelial permeability is essential for development of barrier protective therapeutic strategies. Ongoing studies characterized specific barrier protective mechanisms and identified intracellular targets directly involved in regulation of endothelial permeability. Growing evidence suggests that, although each protective agonist triggers a unique pattern of signaling pathways, selected common mechanisms contributing to endothelial barrier protection may be shared by different barrier protective agents. Therefore, understanding of basic barrier protective mechanisms in pulmonary endothelium is essential for selection of optimal treatment of pulmonary edema of different etiology. This article focuses on mechanisms of lung vascular permeability, reviews major intracellular signaling cascades involved in endothelial monolayer barrier preservation and summarizes a current knowledge regarding recently identified compounds which either reduce pulmonary endothelial barrier disruption and hyperpermeability, or reverse preexisting lung vascular barrier compromise induced by pathologic insults.

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